Antiinflammatory Effects of Orientin-2″-<i>O</i>-Galactopyranoside on Lipopolysaccharide-Stimulated Microglia

Design, synthesis and evaluation of a series of non-steroidal anti-inflammatory drug conjugates as novel neuroinflammatory inhibitors

“…On the contrary, it was suggested that NF-κB and ERK are two unrelated signal pathways in inflammatory responses [54]. We have shown that an ERK specific inhibitor, U0126, did not block NF-κB activation, suggesting that NF-κB activation is not induced by ERK phosphorylation [37]. We therefore suggest that diclofenac-cysteamine conjugate reduces the production of proinflammatory mediators though inhibition of the intracellular signal transduction pathway MAPKs/AP-1.…”

“…The establishment of stable BV-2 cell line expressing NF-κB or AP-1 reporter construct was performed as previously described [37]. In brief, the BV-2 microglia cells stably expressing NF-κB or AP-1 constructs (Cignal™ Lenti Reporters, Qiagen) were seeded on 24 well plates (1 × 10 5 cells/well).…”

“…For the co-culture of microglia/neuron, microglia condition media system was used as described previously [37]. In brief, BV-2 microglia cells were seeded in 6 well cell culture plates.…”

Activation of Nur77 in microglia attenuates proinflammatory mediators production and protects dopaminergic neurons from inflammation‐induced cell death

“…NF-jB reporter assay BV2 cells expressed the NF-jB lentiviral reporter construct (Cignal TM Lenti Reporters; Qiagen, Hilden, Germany) were established as previously described (Zhou et al 2014). About 36 h after the lentiviral transduction, cells were subjected to treatments with LPS for 24 h. In a parallel experiment, BV2 cells were pretreated with CsnB followed by the treatment with LPS for 24 h. Luciferase activities were determined using the luciferase reporter assay system (Promega, Madison, WI, USA).…”

Neuroinflammatory processes in cognitive disorders: Is there a role for flavonoids and n-3 polyunsaturated fatty acids in counteracting their detrimental effects?

“…(Suk et al, 2003;Yeh et al, 2014). In addition, orientin (10-40 µM; extracted from Trollius chinensis), tangeretin (80 µM; a flavonoid from citrus fruit peels) and quercetin (20-30 µM) all reduced LPS-induced NF-κB expression in BV-2 microglial cells (Kang et al, 2013;Shu et al, 2014;Zhou et al, 2014). Of particular interest, it has recently been demonstrated that EGCG (10 µM) attenuates LPS-induced inflammatory and oxidative stress responses by modulating Nrf2 and decreasing Snitrosylation levels of proteins involved in free radical scavenging such as superoxide dismutase (SOD) in BV-2 microglial cells (Qu et al, 2014).…”