Antiinflammatory and Antioxidant Flavonoids and Phenols from Cardiospermum halicacabum (倒地鈴 Dào Dì Líng)

Cheng, Hui‐Ling; Zhang, Lijie; Liang, Yuhan; Hsu, Ya-Wen; Lee, I-Jung; Liaw, Chia‐Ching; Hwang, Syh-Yuan; Kuo, Yao‐Haur

doi:10.1016/s2225-4110(16)30165-1

Cited by 21 publications

(12 citation statements)

References 10 publications

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“…The IC 50 values for the inhibition of NO production by compounds 1 – 4 are all > 50 μM. It has been reported that the known flavonols kaempferol ( 7 ) [ 26 ], quercetin ( 10 ) [ 27 ], and myricetin-3- O - α -L–rhamnopyranoside ( 12 ) [ 28 ] have anti-inflammatory activity, but kaempferol-3-rhamnoside ( 8 ) [ 26 ] and quercetin-3- O - α -L-rhamnoside ( 11 ) [ 29 ] have no anti-inflammatory activity. From the experimental results and literature data, the flavonoids in the title plant have better anti-inflammatory activity than ellagitannins, it may be the active ingredient corresponding to the anti-inflammatory effect of this plant.…”

Section: Resultsmentioning

confidence: 99%

Structural Characterization and Assessment of Anti-Inflammatory and Anti-Tyrosinase Activities of Polyphenols from Melastoma normale

Huang

et al. 2021

Molecules

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Polyphenols, widely distributed in the genus Melastoma plants, possess extensive cellular protective effects such as anti-inflammatory, anti-tyrosinase, and anti-obesity, which makes it a potential anti-inflammatory drug or enzyme inhibitor. Therefore, the aim of this study is to screen for the anti-inflammatory and enzyme inhibitory activities of compounds from title plant. Using silica gel, MCI, ODS C18, and Sephadex LH-20 column chromatography, as well as semipreparative HPLC, the extract of Melastoma normale roots was separated. Four new ellagitannins, Whiskey tannin C (1), 1-O-(4-methoxygalloyl)-6-O-galloyl-2,3-O-(S)-hexahydroxydiphenoyl-β-d-glucose (2), 1-O-galloyl-6-O-(3-methoxygalloyl)-2,3-O-(S)-hexahydroxydiphenoyl-β-d-glucose (3), and 1-O-galloyl-6-O-vanilloyl-2,3-O-(S)-hexahydroxydiphenoyl-β-d-glucose (4), along with eight known polyphenols were firstly obtained from this plant. The structures of all isolates were elucidated by HRMS, NMR, and CD analyses. Using lipopolysaccharide (LPS)-stimulated RAW2 64.7 cells, we investigated the anti-inflammatory activities of compounds 1–4, unfortunately, none of them exhibit inhibit nitric oxide (NO) production, their IC50 values are all > 50 μM. Anti-tyrosinase activity assays was done by tyrosinase inhibition activity screening model. Compound 1 showed weak tyrosinase inhibitory activity with IC50 values of 426.02 ± 11.31 μM. Compounds 2–4 displayed moderate tyrosinase inhibitory activities with IC50 values in the range of 124.74 ± 3.12–241.41 ± 6.23 μM. The structure–activity relationships indicate that hydroxylation at C-3′, C-4′, and C-3 in the flavones were key to their anti-tyrosinase activities. The successful isolation and structure identification of ellagitannin provide materials for the screening of anti-inflammatory drugs and enzyme inhibitors, and also contribute to the development and utilization of M. normale.

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Section: Resultsmentioning

confidence: 99%

Structural Characterization and Assessment of Anti-Inflammatory and Anti-Tyrosinase Activities of Polyphenols from Melastoma normale

Huang

et al. 2021

Molecules

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“…Our results show that all 40% ethanol plant extracts could reduce NO production in both cell lines investigated. In particular, we observed that Epilobium parviflorum and Cardiospermum halicacabum, at 0.1 µg/µL concentration, reduced free radical and NO production only in RAW 264.7 cells, confirming their ability to cope up with oxidative stress, according to literature data [23,39,40]. In order to elucidate the mechanism of action of these 40% ethanol plant extracts, we evaluated their interaction with the A 2A adenosine receptor subtype, having a crucial role in reducing inflammation [41,42].…”

Section: Discussionmentioning

confidence: 71%

Antioxidant and Antiinflammatory Effects of Epilobium parviflorum, Melilotus officinalis and Cardiospermum halicacabum Plant Extracts in Macrophage and Microglial Cells

Merighi

Travagli

Tedeschi

et al. 2021

Cells

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Background: We investigated the phenolic content characterizing different plant extracts from Epilobium parviflorum, Cardiospermum halicacabum, and Melilotus officinalis, their antioxidant, antiinflammatory effects, and their mechanism of action. Methods: plant samples were macerated in 40% ethanol or hot/ cold glycerate and assessed for polyphenols content. The antioxidant activity was investigated by DPPH radical scavenging assay and H2DCFDA test in LPS-stimulated RAW264.7 macrophages and N9 microglial cells. MTS experiments and antiinflammatory properties verified cellular toxicity through NO assay. Interaction with A2A adenosine receptors was evaluated through binding assays using [3H]ZM241385 radioligand. Results: Polyphenols were present in 40% ethanol plant extract, which at 0.1–10 µg/µL achieved good antioxidant effects, with a DPPH radical scavenging rate of about 90%. In LPS-stimulated cells, these plant extracts, at 1μg/μL, did not affect cell vitality, displayed significant inhibition of H2DCFDA and NO production, and inhibited ZM 241385 binding in CHO cells transfected with A2A receptors. RAW 264.7 and N9 cells presented a density of them quantified in 60 ± 9 and 45 ± 5 fmol/mg of protein, respectively. Conclusion: Epilobium parviflorum, Cardiospermum halicacabum, and Melilotus officinalis extracts may be considered a source of agents for treating disorders related to oxidative stress and inflammation.

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“…The OPLS‐DA models of nonsterilized and sterilized soils showed that values of R 2 X (cum) , R 2 Y (cum) , and Q 2 (cum) of OPLS‐DA models improved substantially and were statistically significant ( p < .05) (Table S3). In nonsterilized soil, OPLS‐DA scores and loading plots (Figure 3) showed that soil samples were divided into two groups: (a) the separation of soil samples that were collected from April until August 2016 were contributed by 8.505 min–m/z 359.23956 [M + CH 3 OH‐H] + (putatively identified as ajmaline) (Kumar et al, 2016), 6.909 min–m/z 162.03104 [M + H] + (putatively identified as indole‐3‐carboxylic acid) (Cheng et al, 2013), and 7.324 min–m/z 398.26883 [M + H] + (putatively identified as phytosphingosine‐1‐phosphate) (Singh, MacKenzie, Girnun, & Del Poeta, 2017) and (b) the soil samples that were collected from October 2016 until January 2017 were discriminated by 9.445 min–m/z 485.11102 [M + Na] + , putatively identified as tectoridin (Alara, Abdurahman, Ukaegbu, Azhari, & Kabbashi, 2018). Meanwhile, the soil samples collected in September 2016 were plotted away and were regarded as outlier.…”

Section: Resultsmentioning

confidence: 99%

The dynamic linkage between phytotoxicity and metabolites in Wedelia trilobata soils

Azizan

Ibrahim

Yusoff

et al. 2021

Weed Biology and Management

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The phytotoxicity of soils under Wedelia trilobata has been described, but the dynamic between phytotoxic and metabolites in W. trilobata soil and the restriction posed by soil properties have been poorly addressed. In this study, the phytotoxic variation of W. trilobata soil in response to the changes in soil properties and soil metabolomics was determined at monthly intervals for 11 months using bioassays and metabolomic approaches. Strong variability in soil properties and obvious temporal variations in the soil phytotoxicity were observed over the 11‐month period. Among the soil properties that were analyzed, soil organic matter, electrical conductivity, total nitrogen, and soil nutrients presented the most contribution in differentiating soils according to time of sampling and strength of phytotoxicity. Nonsterilized soil exerted stronger percentage inhibition of seed germination (IG) of lettuce, whereas the percentage inhibition of shoot height (SH) and root length (RL) of lettuce was higher in sterilized soil. Decline in the overall phytotoxicity was clearly observed in nonsterilized soil, suggesting the role of soil microbes in mediating the soil phytotoxic effect of W. trilobata, albeit the negative correlation. Putative metabolites contributing to the discrimination of soils according to time of sampling and strong correlation with percentage inhibition (%) of IG, SH, and RL were identified and discussed. Overall, the results showed that the phytotoxicity of soils under W. trilobata varied throughout the 11 months of study and can be restricted by the changes in soil properties.

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Antiinflammatory and Antioxidant Flavonoids and Phenols from Cardiospermum halicacabum (倒地鈴 Dào Dì Líng)

Cited by 21 publications

References 10 publications

Structural Characterization and Assessment of Anti-Inflammatory and Anti-Tyrosinase Activities of Polyphenols from Melastoma normale

Structural Characterization and Assessment of Anti-Inflammatory and Anti-Tyrosinase Activities of Polyphenols from Melastoma normale

Antioxidant and Antiinflammatory Effects of Epilobium parviflorum, Melilotus officinalis and Cardiospermum halicacabum Plant Extracts in Macrophage and Microglial Cells

The dynamic linkage between phytotoxicity and metabolites in Wedelia trilobata soils

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