Antihyperuricemia, Antioxidant, and Antibacterial Activities of Tridax procumbens L.

Abstract

Tridax procumbens L. is a medicinal plant and used as a drink to treat bronchial catarrh, diarrhea, dysentery and liver diseases. In this study, we evaluated the potential use of T. procumbens to treat hyperuricemia, oxidative stress, and bacterial infection. Ethyl acetate extract of this plant was separated to different fractions by column chromatography (CC) using chloroform and methanol as eluents and subjected to xanthine oxidase (XO) inhibitory, antioxidant, and antibacterial assays. The results showed that the F45–47 fraction exhibited the strongest XO inhibitory activity (IC50 = 133.17 µg/mL), while the F48–50 fraction possessed maximum antioxidant activity assessed by DPPH (2,2-diphenyl-2-picrylhydrazyl) and ABTS (2,2’-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) assays (IC50 = 0.51 and 1.04 mg/mL, respectively). In addition, the F4–5 fraction presented the most effective inhibition on the growth of Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Proteus mirabilis. Gas chromatography-mass spectrophotometry (GS-MS) and liquid chromatography-electrospray ionization-mass spectrophotometry (LC-ESI-MS) results revealed that fatty acids, glycerides, and flavonoids were the major compounds of the F45–47 fraction. Glycerides, triose sugar alcohols, and fatty acids were dominant compounds of the F48–50 fraction, while sterols were principal components of the F4–5 fraction. This study indicated that T. procumbens had potent inhibitory effects on XO inhibitory, antioxidant, and antibacterial activities. These biological activities may be attributed to the presence of fatty acids, flavonoids, and sterols in this plant. It is suggested that T. procumbens can be utilized as a healthy source to develop beverages and foods to treat antihyperuricemia, oxidative stress, and bacterial infection.

Anticancer, Antibacterial, and Phytochemicals Derived From Extract of Aerva javanica (Burm.f.) Juss. ex Schult. Grown Naturally in Saudi Arabia

“…A previous study showed that aqueous extraction is not as potent as extractions using organic solvents. Andriana et al (2019) reported that hot water extract of Tridax procumbens L. showed no inhibition activity against the tested microbes. This study confirmed earlier observations that either polar or nonpolar solvents had the capacity to produce a large number of bioactive molecules (Sujatha & Suresh, 2013;Tiwari, Kumar, Kaur, Kaur, & Kaur, 2011).…”

Biological Activities and Chemical Constituents of Essential Oils from Piper cubeba Bojer and Piper nigrum L.

“…The xanthine oxidase (XO) inhibitory activity was assayed spectrophotometrically in vitro under aerobic condition according to the method reported previously [46,47]. Briefly, a volume of 50 µL sample or standard was mixed with 35 µL of 70 mM phosphate buffer (pH = 7.5), and 30 µL of fresh xanthine oxidase solution (0.01 units/mL in 70 mM phosphate buffer, pH = 7.5) in a 96-well microplate.…”

“…Chemical composition of P. cubeba and P. nigrum EOs detected in GC-MS system both for volatile and non-volatile components were confirmed by LC-ESI-MS system (Thermo Fisher Scientific TM , LTQ XLTM, Ion Trap Mass Spectrometer, Tokyo, Japan) [47]. The column used for the LC system was a JASCO J-Pak Symphonia C18 (250 mm × 4.6 mm × 5 μm).…”

Comprehensive Fractionation of Antioxidants and GC-MS and ESI-MS Fingerprints of Celastrus hindsii Leaves

“…Antioxidant activity of extracts and isolated fractions were estimated by DPPH free radical scavenging method described by [15]. In details, a mixture contained 0.5 mL of each sample, 0.25 mL of 0.5 nM DPPH, and 0.5 mL of 0.1 M acetate buffer (pH 5.5) was prepared and placed in the dark for 30 min at ambient conditions.…”