Antihypertensive peptides from curd

Abstract

Introduction:Curd (Dadhi) peptides reduce hypertension by inhibiting angiotensin converting enzyme (ACE) and serum cholesterol. Peptides vary with bacterial species and milk type used during fermentation.Aim:To isolate and assay the antihypertensive peptides, before and after digestion, in two commercially available curd brands in Sri Lanka.Materials and Methods:Whey (Dadhi Mastu) separated by high-speed centrifugation was isolated using reverse-phase-high- performance liquid chromatography (HPLC). Eluted fractions were analyzed for ACE inhibitory activity using modified Cushman and Cheung method. Curd samples were subjected to enzymatic digestion with pepsin, trypsin, and carboxypeptidase-A at their optimum pH and temperature. Peptides isolated using reverse-phase-HPLC was assayed for ACE inhibitory activity.Results:Whey peptides of both brands gave similar patterns (seven major and five minor peaks) in HPLC elution profile. Smaller peptides concentration was higher in brand 1 and penta-octapeptides in brand 2. Pentapeptide had the highest ACE inhibitory activity (brand 2–90% and brand 1–73%). After digestion, di and tri peptides with similar inhibitory patterns were obtained in both which were higher than before digestion. Thirteen fractions were obtained, where nine fractions showed more than 70% inhibition in both brands with 96% ACE inhibition for a di-peptide.Conclusion:Curd has ACE inhibitory peptides and activity increases after digestion.

Anticancer potential of bioactive peptides from animal sources

“…In this context, bioactive peptides are increasingly being considered as good drug candidates for cancer therapeutic applications. A growing body of peptides from natural animal sources has been demonstrated to possess physiological functions, such as immunomodulatory (6), antimicrobial (7), antihypertensive (8), antithrombotic (9), anticancer (10), antioxidative (11), and cholesterol-lowering activities (12). However, this review focuses on bioactive peptides from animal sources that may specifically target cancer cells and could consequently serve as anticancer agents that are less toxic to normal tissues.…”

Effect of animal-sourced bioactive peptides on the in vitro development of mouse preantral follicles

“…During the promising investigation of natural antioxidants, BAPT have attracted increasing interest as prominent candidates. As a consequence, various products or ingredients with BAPT as fundamental constituents were commercially available and marketed globally, which were based on the specific characteristics of BAPT including anticancer [38], antimicrobial [39], antihypertensive [40], antithrombotic [41], antioxidative [42], immunomodulatory [43] and cholesterol-lowering properties [44]. These products are enriched with BAPT either by modification of the usual manufacturing processes or by simply adding the BAPT during product preparation.…”

Purification and characterization of angiotensin-I-converting enzyme inhibitory peptides isolated from whey proteins of milk fermented with Lactobacillus plantarum QS670

“…Compared with nonmilk sources, the ACEI activity of GA (63.71%) was higher than that of ACEI activity of peptide (54%) generated from porcine skeletal muscle proteins by the action of meat-borne Lactobacillus (Castellano et al, 2013). Wu et al (2019) and Dabarera et al (2015) isolated more efficient ACEI peptides from Lactobacillus delbrueckii QS306-fermented milk (93.86%) and curd (96%). Although ACEI activity of GA is not the most outstanding, its small size led to a low degree of intermolecular polymerization and extended molecular state, compared with the crimp state of macromolecular substance, giving the ACEI peptide GA good solubility.…”