Antihypertensive Effects of Roselle-Olive Combination in L-NAME-Induced Hypertensive Rats

Abdel-Rahman, Rehab F.; Hessin, Alyaa F.; Abdelbaset, Marwan; Ogaly, Hanan A.; Abd‐Elsalam, Reham M.; Hassan, Samah

doi:10.1155/2017/9460653

Cited by 49 publications

(57 citation statements)

References 99 publications

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“…The immune-histochemical analysis of AR and PCNA were done according to Abdel-Rahman et al (2017). The tissue specimens were deparaffinized and rehydrated then pretreated with citrate buffer pH 6 for 20 min for antigen retrieval.…”

Section: Methodsmentioning

confidence: 99%

Olive leaves extract attenuates type II diabetes mellitus-induced testicular damage in rats: Molecular and biochemical study

Soliman

Saeedan

Abdel-Rahman

et al. 2019

Saudi Pharmaceutical Journal

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Diabetes mellitus (DM) has emerged as a public healthcare problem. Sustained hyperglycemia has been linked with many complications including impaired male fertility Olive tree ( Olea europaea L.) leaves have been extensively used in traditional remedies worldwide to control blood glucose level in DM. In this study, the beneficial role of olive leaves extract (OLE) was investigated to combat diabetes-induced adverse effect on testicular tissues. Thirty male Wistar rats were divided into 5 equal groups: normal control group, streptozotocin (STZ)-diabetic group and diabetic groups which were given glibenclamide (GLB) or OLE at 250 and 500 mg/kg for 9 weeks to investigate the efficiency of olive leaves extract (OLE) in reducing the deleterious effect of diabetes on the reproductive system of male rats. Rats were checked for serum glucose, insulin, testosterone and gonadotropins. Also, testicular antioxidants, epididymal sperm characteristics and testicular histopathology were assessed. Expression of the testicular steroidogenic enzymes, cholesterol side-chain cleavage enzyme (P450 scc) and 17β-hydroxysteroid dehydrogenase (17β-HSD) was examined. Moreover, androgen receptor and proliferating cell nuclear antigen (PCNA) protein immunohistochemistry were assessed in testes. STZ-induced diabetes significantly increased serum glucose. However, STZ significantly decreased serum levels of insulin, testosterone, follicle stimulating hormone (FSH) and luteinizing hormone (LH). Marked reductions in testicular antioxidants with elevated malondialdehyde (MDA) parallel with deterioration of the testicular histoarchitecture and epididymal sperm characteristics were recorded. Administration of GLB or OLE (250 and 500 mg/kg) resulted in a significant recovery of the above mentioned parameters in STZ-diabetic rats. Interestingly, OLE shows greater glycemic improvement and testicular protection than GLB with the highest percentage protection exhibited by the OLE high dose. Furthermore, OLE significantly induced testicular steroidogenesis in diabetic rat as evidenced by elevated P450 scc and 17β-HSD mRNA expression. The study proves that OLE possesses a potential protective role against diabetes-induced reproductive disorders, which may be due to its antioxidant activity and its ability to normalize testicular steroidogenesis.

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Section: Methodsmentioning

confidence: 99%

Olive leaves extract attenuates type II diabetes mellitus-induced testicular damage in rats: Molecular and biochemical study

Soliman

Saeedan

Abdel-Rahman

et al. 2019

Saudi Pharmaceutical Journal

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“…The immunohistochemical analysis of caspase-3 and p53 content of the hepatic and cardiac tissues were performed according to the methods described by Abdel-Rahman et al. (2017).…”

Section: Methodsmentioning

confidence: 99%

Protective effect of some natural products against chemotherapy-induced toxicity in rats

Abdallah

Abdel-Rahman

Awdan

et al. 2019

Heliyon

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Aim There is a great interest in combining anticancer drugs with natural products aiming at maximizing their efficacy while minimizing systemic toxicity. Hence, the present study was constructed aiming to investigate the protective potential of three natural products, 1,8-cineole an essential oil from Artemisia herba alba , exopolysaccharide (EPS) from locally identified marine streptomycete, and ellagic acid (EA), against chemotherapy-induced organ toxicity. Methods Isolation, production and characterization of EPS from marine streptomycete was done. Animals were allocated into five groups, GP1: normal control, GP2: cyclophosphamide (CYC), GP3: 1,8-cineole + CYC, GP4: EPS + CYC, GP4: EA + CYC. All drugs were administered orally 1 week before and concomitantly with CYC. Electrocardiography (ECG) analysis, liver enzymes (ALT and AST), cardiac serum markers (LDH and CK), oxidative stress biomarkers in hepatic and cardiac tissues (GSH and MDA), TGF-β1 and histopathological examination of hepatic and cardiac tissues were executed. Results The isolated stain produced EPS was identified as Streptomyces xiamenensis. EPS contains uronic, sulphate groups and different monosugars with Mw 4.65 × 10 4 g/mol and showed antioxidant activity against DPPH. Pretreatment of rats with 1,8-cineole, EPS and EA improved ECG abnormalities, decrease serum markers of hepato- and cardiotoxicity, prevent oxidative stress and decrease TGF-β1 in liver and heart tissues. Conclusion The present results demonstrate the hepatoprotective and cardioprotective effects of the above-mentioned natural products against CYC organ toxicity.

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“…This finding was consistent with previous studies by Adaramoye et al [38] and Berkban et al [39] who also observed normal levels of NO in heart and kidney of L-NAME treated rats. Abdel-Raham et al [40] proposed that eNOS protein expression may be up-regulated in L-NAME treated rats as a counter-regulatory mechanism to compensate for increased BP. On the other hand Luvarà et al [41] showed that chronic L-NAME administration was associated with the induction of iNOS expression both at mRNA and protein level.…”

Section: Discussionmentioning

confidence: 99%

Antihypertensive effects of the hydro-ethanol extract of Senecio serratuloides DC in rats

Tata

Sewani-Rusike

Oyedeji

et al. 2019

BMC Complement Altern Med

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Background Senecio serratuloides DC is used in folk medicine for treating hypertension, skin disorders, internal and external sores, rashes, burns and wounds. This study aimed at investigating the antihypertensive effects of the hydroethanol extract of S. serratuloides (HESS) in N-Nitro-L-arginine methyl ester (L-NAME) induced hypertension in rats. Methods: Acute toxicity of HESS was first determined to provide guidance on doses to be used in this study. Lorke’s method was used to determine safety of the extract in mice. Female Wistar rats were treated orally once daily with L-NAME (40 mg/kg) for 4 weeks and then concomitantly with L-NAME (20 mg/kg) and plant extract (150 and 300 mg/kg), captopril (20 mg/kg) or saline as per assigned group for 2 weeks followed by a 2-week period of assigned treatments only. Blood pressure was monitored weekly. Lipid profile, nitric oxide, renin and angiotensin II concentrations were determined in serum while mineralocorticoid receptor concentration was quantified in the kidney homogenate. Nitric oxide (NO) concentration was determined in serum and cardiac histology performed. Results HESS was found to be non-toxic, having a LD 50 greater than 5000 mg/kg. Blood pressure increased progressively in all animals from the second week of L-NAME treatment. HESS treatment significantly and dose-dependently lowered systolic blood pressure ( p < 0.001), diastolic blood pressure ( p < 0.01), low density lipoprotein cholesterol ( p < 0.01) and triglycerides ( p < 0.01). It significantly prevented L-NAME induced decrease in serum angiotensin II ( p < 0.01), high density lipoprotein cholesterol ( p < 0.001) and serum nitric oxide concentrations ( p < 0.001). HESS also significantly ( p < 0.01) prevented collagen deposition in cardiac tissue. Conclusion The hydro-ethanol extract of Senecio serratuloides showed antihypertensive, antihyperlipidemic and cardioprotective effects in rats thus confirming its usefulness in traditional antihypertensive therapy and potential for antihypertensive drug development.

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Antihypertensive Effects of Roselle-Olive Combination in L-NAME-Induced Hypertensive Rats

Cited by 49 publications

References 99 publications

Olive leaves extract attenuates type II diabetes mellitus-induced testicular damage in rats: Molecular and biochemical study

Olive leaves extract attenuates type II diabetes mellitus-induced testicular damage in rats: Molecular and biochemical study

Protective effect of some natural products against chemotherapy-induced toxicity in rats

Antihypertensive effects of the hydro-ethanol extract of Senecio serratuloides DC in rats

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