Antihypertensive effect of insect cells: In vitro and in vivo evaluation

Staljanssens, Dorien; Camp, John Van; Herregods, Griet; Dhaenens, Maarten; Deforce, Dieter; Voorde, Johan Van de; Smagghe, Guy

doi:10.1016/j.peptides.2010.08.011

Cited by 19 publications

(14 citation statements)

References 45 publications

(1 reference statement)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Prior to QSAR modeling, proteins have to be enzymatically hydrolyzed and isolated using bioassay guided fractionation; the peptides are then identified by MS/MS spectrometry and subsequently synthesized for structure-activity relationship. Several studies have been conducted using this model of rats to screen potential antihypertensive peptides from different food sources (Cheng et al, 2008;Contreras, Carrón, Montero, Ramos & Recio, 2009;Girgih, Udenigwe, Hasan, Gill & Aluko, 2013;Hiwatashi et al, 2010;Lee, Qian & Kim, 2010;Miguel, Gómez-Ruiz, Recio & Aleixandre, 2010;Quiñones et al, 2010;Staljanssens et al, 2011;. The antihypertensive properties of peptides have recently been reviewed and two main routes (oral gavage and intravenous administration) are employed for administering these peptides to the rats.…”

Section: Structure-function Relationship Of Bioactive Peptidesmentioning

confidence: 99%

“…From evaluating the kinetics parameters it is clear the peptides from hemp seed have the potential to act as effective ACE and renin inhibitors. Quiñones et al, 2010;Staljanssens et al, 2011) and immunomodulatory Regazzo et al, 2010;Seil, Nagant, Dehaye, Vandenbranden & Lensink, 2010) agents. Other works have also produced food protein-derived peptides with antibacterial (Hartmann & Meisel, 2007;Korhonen & Pihlanto, 2006;Madureira, Tavares, Gomes, Pintado & Malcata, 2010), anticancer (Bhutia & Maiti, 2008;Hoskin & Ramamoorthy, 2008;Wu et al, 2009), antidiabetic Huang & Wu, 2010;Zhu et al, 2010) and anti-inflammatory Haidet, Cifarelli, Trucco & Luppi, 2009;Young, Fan & Mine, 2010) properties.…”

Section: Transition Statementmentioning

confidence: 99%

See 1 more Smart Citation

Structural and functional characterization of hemp seed (Cannabis sativa L.) protein-derived antioxidant and antihypertensive peptides

Girgih

Malomo

et al. 2014

Journal of Functional Foods

240

142

View full text Add to dashboard Cite

The aim of this work was to produce enzymatic hemp seed protein hydrolysates (HPH) followed by bioassay guided fractionation to identify antioxidant and antihypertensive peptides. Therefore, simulated gastrointestinal digestion of isolated hemp seed proteins was conducted using consecutive actions of pepsin and pancreatin to produce HPH, which was then separated by membrane ultrafiltration to obtain peptide sizes of <1, 1-3, 3-5, and 5-10 kDa. Evaluation of HPH and its membrane fractions for antioxidant and antihypertensive properties showed that they significantly (P<0.05) scavenged radicals, reduced and strongly chelated metal ions as well as inhibited lipid oxidation. During a 24-hr test, the HPH reduced systolic blood pressure (SBP) of spontaneously hypertensive rats (SHR) after oral administration by a maximum of -30 mmHg when compared to -15 mmHg for the membrane fractions.To reduce production cost, hemp seed protein meal (HPM) was directly hydrolyzed to a protein hydrolysate (HMH) and was shown to also reduce SBP during 4-8 weeks of dietary feeding. The attenuation of SBP correlated to suppressed plasma levels (0.047-0.059 U/mL and 0.040-0.054 µg/mL) of angiotensin converting enzyme (ACE) and renin, respectively, when compared to the control rats (0.123 U/mL and 0.151 µg/mL). A total of 23 peptides were identified to be present in the HPH. WVYY and PSLPA showed superior in vitro antioxidant properties, while ACE activity was inhibited by WYT (89%), WVYY (91%) and PSLPA (90%). Renin activity was inhibited by WYT (77%), SVYT (87%) and IPAGV (75%). However, oral administration to SHR showed that the pentapeptides (PSLPA and IPAGV) were more effective SBP-reducing agents (-40 mm Hg) when compared to the tri-(-13 mmHg) and tetrapeptides (-36 mmHg). These results

show abstract

Section: Structure-function Relationship Of Bioactive Peptidesmentioning

confidence: 99%

Section: Transition Statementmentioning

confidence: 99%

Structural and functional characterization of hemp seed (Cannabis sativa L.) protein-derived antioxidant and antihypertensive peptides

Girgih

Malomo

et al. 2014

Journal of Functional Foods

240

142

View full text Add to dashboard Cite

show abstract

“…Drosulfakinins and sulfakinins of other insects are related to members of the vertebrate CCK peptide family, based on similarities in amino acid sequence and the requirement of a sulfated tyrosyl residue for bioactivity (Nachman et al, 1986a,b, 1988; Nichols et al, 1988; Staljanssens et al, 2011). Also the DSK receptors (CG6857 and CG6881) are considered related to the gastrin/CCK receptors, based on similarities in amino acid sequence and gene organization (Kubiak et al, 2002; Hauser et al, 2006; Janssen et al, 2008; Staljanssens et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Insulin-Producing Cells in the Drosophila Brain also Express Satiety-Inducing Cholecystokinin-Like Peptide, Drosulfakinin

2012

View full text Add to dashboard Cite

Regulation of meal size and assessing the nutritional value of food are two important aspects of feeding behavior. The mechanisms that regulate these two aspects have not been fully elucidated in Drosophila. Diminished signaling with insulin-like peptides Drosophila insulin-like peptides (DILPs) affects food intake in flies, but it is not clear what signal(s) mediates satiety. Here we investigate the role of DILPs and drosulfakinins (DSKs), cholecystokinin-like peptides, as satiety signals in Drosophila. We show that DSKs and DILPs are co-expressed in insulin-producing cells (IPCs) of the brain. Next we analyzed the effects of diminishing DSKs or DILPs employing the Gal4-UAS system by (1) diminishing DSK-levels without directly affecting DILP levels by targeted Dsk-RNAi, either in all DSK-producing cells (DPCs) or only in the IPCs or (2) expressing a hyperpolarizing potassium channel to inactivate either all the DPCs or only the IPCs, affecting release of both peptides. The transgenic flies were assayed for feeding and food choice, resistance to starvation, and for levels of Dilp and Dsk transcripts in brains of fed and starved animals. Diminishment of DSK in the IPCs alone is sufficient to cause defective regulation of food intake and food choice, indicating that DSK functions as a hormonal satiety signal in Drosophila. Quantification of Dsk and Dilp transcript levels reveals that knockdown of either peptide type affects the transcript levels of the other, suggesting a possible feedback regulation between the two signaling pathways. In summary, DSK and DILPs released from the IPCs regulate feeding, food choice and metabolic homeostasis in Drosophila in a coordinated fashion.

show abstract

“…Cell lines used in this study were also used as model systems for testing of various xenobiotics, e.g. WBF344 for food and perfume additives 13 , RK13 for mycotoxins 14 , and the cell line Sf21 for testing of insect cell extracts 15 or novel insecticides 16 . Manji and Friesen 17 and Tseng et al 18 studied induction of apoptosis and Takahashi et al 19 cell cycle arrest induced by radiation in SF21 cell line.…”

Section: Discussionmentioning

confidence: 99%

İnsektisit Endosülfana Maruz Kalan Hayvansal ve Böcek Kökenli Hücre Kültürlerindeki Proliferasyon ve LDH Fazlalığı

Kovalkovičová¹,

Pistl²,

Csank³

et al. 2013

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

SummaryIn the present study three different cell cultures, derived from rabbit kidney (RK13), rat liver (WBF344) and insect origin (Sf21), were used to examine the cytotoxic effect of the insecticide endosulfan. Cytotoxicity was determined on the basis of cell proliferation activity; the cellular damage was assessed by evaluation of cytopathic effect and lactate dehydrogenase (LDH) leakage. Endosulfan treatment suppressed proliferative activity in cell cultures as follows: insect Sf21 cells (10 -2 M (P<0.05) miktarında belirlendi. Bu sonuçlar endosülfan maruziyetine karşı hücre tipine bağlı duyarlılığı işaret etmektedir. Endosülfan, memeli hücre kültürüne göre böcek kökenli hücre kültürlerindeki hücre sayılarında daha belirgin bir düşüşe neden olsa da LDH fazlalığı ve hücredeki mikroskobik hasar belirteçleri en belirgin olarak karaciğer hücrelerinde görülmektedir.

show abstract

Antihypertensive effect of insect cells: In vitro and in vivo evaluation

Abstract: 38In this study, we investigated the in vitro ACE inhibitory and in vivo antihypertensive effect 39 of insect cell extracts. The IC 50 of three insect cell lines from different type and insect species

Cited by 19 publications

References 45 publications

Structural and functional characterization of hemp seed (Cannabis sativa L.) protein-derived antioxidant and antihypertensive peptides

Structural and functional characterization of hemp seed (Cannabis sativa L.) protein-derived antioxidant and antihypertensive peptides

Insulin-Producing Cells in the Drosophila Brain also Express Satiety-Inducing Cholecystokinin-Like Peptide, Drosulfakinin

İnsektisit Endosülfana Maruz Kalan Hayvansal ve Böcek Kökenli Hücre Kültürlerindeki Proliferasyon ve LDH Fazlalığı

Contact Info

Product

Resources

About