Antihypertensive Effect of Alkaline Protease Hydrolysate of Whey Protein on Blood Pressure in Spontaneously Hypertensive Rats.

Eto, Yoshiharu; Ito, Tomomi; Nishioka, Shigeko

doi:10.4327/jsnfs.52.301

Cited by 6 publications

(4 citation statements)

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“…The activity of myosin B, which amounts to 50% of meat protein (Hattori, 1996), was especially noteworthy, and supports the suggestion that meat is a biologically active food. Since some reports have indicated the hypotensive activities of ACE inhibitory hydrolysates, derived from fish (Sugiyama et al, 1991), whey protein (Eto et al, 1999), hen's eggs (Yoshii et al, 1999), and porcine myosin and water-insoluble protein (Nakashima et al, 2002), after oral administration to spontaneously hypertensive rats, porcine protein hydrolysates prepared here were expected to be useful as hypotensive foods without separation of the active peptides. ACE inhibitory peptides from actin (Yokoyama et al, 1992) and myosin (Arihara et al, 2001) have been reported…”

Section: Angiotensin I-converting Enzyme Inhibitory Activities Of Den...mentioning

confidence: 97%

“…Di-and tripeptides have been reported to be resistant to digestive enzymes and to be absorbed in the intestine (Adibi et al, 1969;Craft et al, 1968;Li et al, 1999). Recently, several studies (Yokoyama et al, 1992;Matsumoto et al, 1994;Eto et al, 1999;Ohta et al, 1999;Yoshii et al, 1999;Fujita et al, 2000;Arihara et al, 2001) have been conducted to design digestive enzyme-resistant peptides made by microbial proteases. Our results indicate that a normal diet like porcine meat might generate ACE inhibitory peptides by the action of digestive enzymes.…”

Section: Angiotensin I-converting Enzyme Inhibitory Activities Of Pep...mentioning

confidence: 99%

“…ACE catalyzes the conversion of angiotensin I to angiotensin II, which causes hypertension, and inactivates bradykinin, which is antihypertensive. ACE inhibitory peptides have been studied in many other foods, such as dried bonito (Yokoyama et al, 1992), sardine muscle (Suetsuna and Osajima, 1986;Kawamura et al, 1989;Sugiyama et al, 1991;Matsui et al, 1993), krill (Kawamura et al, 1992), tuna muscle (Kohama et al, 1988), casein (Maruyama et al, 1985), sake lees (Saito et al, 1994), oysters (Matsumoto et al, 1994), whey protein (Eto et al, 1999), hen's eggs (Yoshii et al, 1999), and chum salmon (Ohta et al, 1999). ACE inhibitory peptides from chicken breast muscle (Fujita et al, 2000) and porcine skeletal muscle, biceps femoris, (Arihara et al, 2001;Nakashima et al, 2002) were reported.…”

Section: Introductionmentioning

confidence: 99%

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Angiotensin I-converting Enzyme Inhibitory Activities of Porcine Skeletal Muscle Proteins Following Enzyme Digestion

Katayama¹,

Fuchu²,

Sakata³

et al. 2003

Asian Australas. J. Anim. Sci

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Inhibitory activities against angiotensin I-converting enzyme (ACE) of enzymatic hydrolysates of porcine skeletal muscle proteins were investigated. Myosin B, myosin, actin, tropomyosin, troponin and water-soluble proteins extracted from pork loin were digested by eight kinds of proteases, including pepsin, α-chymotrypsin, and trypsin. After digestion, hydrolysates produced from all proteins showed ACE inhibitory activities, and the peptic hydrolysate showed the strongest activity. In the case of myosin B, the molar concentration of peptic hydrolysate required to inhibit 50% of the activity increased gradually as digestion proceeded. The hydrolysates produced by sequential digestion with pepsin and α-chymotrypsin, pepsin and trypsin or pepsin and pancreatin showed weaker activities than those by pepsin alone, suggesting that ACE inhibitory peptides from peptic digestion might lose their active sequences after digestion by the second protease. However, the hydrolysates produced by sequential digestion showed stronger activities than those by α-chymotrypsin, trypsin or pancreatin alone. These results suggested that the hydrolysates of porcine meat were able to show ACE inhibitory activity, even if they were digested in vivo, and that pork might be a useful source of physiologically functional factors.

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Section: Angiotensin I-converting Enzyme Inhibitory Activities Of Den...mentioning

confidence: 97%

Section: Angiotensin I-converting Enzyme Inhibitory Activities Of Pep...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Angiotensin I-converting Enzyme Inhibitory Activities of Porcine Skeletal Muscle Proteins Following Enzyme Digestion

Katayama¹,

Fuchu²,

Sakata³

et al. 2003

Asian Australas. J. Anim. Sci

View full text Add to dashboard Cite

show abstract

“…There are several reports on the antihypertensive effects of food protein in relation to the functional peptides in rat and human feeding studies (Suestna and Osajima, 1989;Sekiya et al, 1992;Yamamoto et al, 1994;Nakamura et al, 1996;Hata, et al, 1996;Eto et al, 1999). In SHR experiment, SBP were measured at 0, 3, 6 h after 150 mg/kg and 300 mg/kg oral administration.…”

Section: Resultsmentioning

confidence: 99%

Production of Angiotensin-I Converting Enzyme Inhibitory Hydrolysates from Egg Albumen

Kim¹,

Ham²,

Jeong³

et al. 2003

Asian Australas. J. Anim. Sci

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ACE (Angiotensin-I converting enzyme) inhibitory peptides derived from foods are thought to suppress high blood pressure by inhibiting ACE. We tried to make efficient production of the ACE inhibitory hydrolysate from egg albumen. A hydrolysate digested by neutrase presented the highest ACE inhibitory activity (IC 50 value=256.35 µg/ml) and the proper proteolysis was occurred by 1.0% enzyme addition and 4 h incubation at 47°C. Antihypertensive effect of neutrase hydrolysate was investigated in spontaneously hypertensive rats (SHR, n=5). Systolic blood pressure (SBP) was decrease by 6.88% (-14.14 mmHg, p<0.05) at 3 h after oral administration of 300 mg/kg body weight, and by 13.33% (-27.72 mmHg, p<0.05) by emulsified hydrolysate. These results showed that it is very effective to utilize egg albumen as a protein source for the production of ACE inhibitory peptides. However, further studies are required to investigate the methods to increase recovery yield and the isolation of active peptide is necessary for determining its sequence responsible for ACE inhibitory activity.

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Health benefits of whey proteins

Sharma

Shah

2010

Nutrafoods

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Antihypertensive Effect of Alkaline Protease Hydrolysate of Whey Protein on Blood Pressure in Spontaneously Hypertensive Rats.

Cited by 6 publications

References 0 publications

Angiotensin I-converting Enzyme Inhibitory Activities of Porcine Skeletal Muscle Proteins Following Enzyme Digestion

Angiotensin I-converting Enzyme Inhibitory Activities of Porcine Skeletal Muscle Proteins Following Enzyme Digestion

Production of Angiotensin-I Converting Enzyme Inhibitory Hydrolysates from Egg Albumen

Health benefits of whey proteins

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