Antihypertensive and bovine plasma oxidation-inhibitory activities of spent hen meat protein hydrolysates

Abstract

Hydrolysis of spent hen meat proteins with pepsin and pepsin 1 pancreatin yielded SPH-P and SPH-PPc hydrolysates, respectively. The hydrolysates dose-dependently inhibited activities of human recombinant renin (IC 50 0.34 and 0.52 mg/mL, respectively) and angiotensin I-converting enzyme (ACE, IC 50 0.64 and 0.42 mg/mL, respectively). The hydrolysates had lower cysteine and tryptophan contents but similar contents of other amino acids when compared with the parent spent hen protein isolate. SPH-P, but not SPH-PPc, dose-dependently increased the sulfhydryl content and ferric reducing antioxidant capacity of bovine plasma oxidized with H 2 O 2 , which dem-

Dose-Related Antihypertensive Properties and the Corresponding Mechanisms of a Chicken Foot Hydrolysate in Hypertensive Rats

“…Then, these peptides were rapidly metabolized into inactive products leading to the subsequent reduction in activity, especially for 85 mg/kg bw Hpp11, with no apparent antihypertensive by 8 h post-administration. A similar antihypertensive pattern was recently described by Udenigwe et al using by-product hen meat protein hydrolysates [ 43 ]. These results showed that at relatively low doses, Hpp11 was able to reduce the SBP in the same manner as Captopril, demonstrating its potential applicability in HTN treatment.…”

Spent Hen Protein Hydrolysate with Good Gastrointestinal Stability and Permeability in Caco-2 Cells Shows Antihypertensive Activity in SHR

“…The extracted proteins in this study contained mainly myofibrillar proteins as reported previously [ 29 , 30 ]. The major protein bands include myosin heavy chain (~220 kDa), actin (42 kDa), and myosin light chain (15–25 kDa) ( Figure 1 A) [ 37 , 55 , 56 ]. These proteins were cleaved into peptides below 10 kDa after the hydrolysis and more than half of peptides were below 3 kDa ( Figure 1 A,B).…”

“…Thus, finding a more sustainable solution for spent hen utilization which also yields additional value to the egg industry is highly relevant. Previous work on spent hens has mainly included extraction and characterization of spent hen proteins [ 28 , 30 ], fabrication of nanomaterials [ 30 , 31 , 32 ], and preparation of bioactive peptides [ 29 , 33 , 34 , 35 , 36 , 37 ]. We have previously prepared a spent hen muscle protein hydrolysate (SPH) that exhibited anti-inflammatory activity in young rodents [ 34 ]; a few anti-inflammatory peptides have been identified therein [ 29 ].…”

Recovery of Natural Antioxidants from Agro-Industrial Side Streams through Advanced Extraction Techniques

“…For the extraction methods of target compounds from these types of materials more attention must be payed to other details, than in the case of plant-based materials, due to their complex matrix and the possibility to obtain more diversified categories of wastes (skin, blood, shells, etc.). Most of the studies describes a combination of thermal, mechanical, chemical and enzymatic methods, such as blending followed by extraction with alkali/acid and hydrolysis with pepsin/pancreatin for the obtaining of peptides from spent hen meat hydrolysate [134] or homogenization followed by enzymatic hydrolysis [135], homogenization, hydrolysis, and ultrafiltration for obtaining carnosine from chicken meat [136].…”