Antihypertensive and antimicrobial bioactive peptides from milk proteins

Haque, Emily; Chand, Rattan

doi:10.1007/s00217-007-0689-6

Cited by 157 publications

(101 citation statements)

References 77 publications

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“…2 Furthermore, protein hydrolysates are recognized as the best, cheap, accessible, and safe source of AMPs. 3,4 However, the existing chromatographic techniques, which take too much time for discovering and high-throughput screening (HTS) of AMPs, hamper the industrial development of AMPs. 5,6 Biochromatography 7,8 as an emerging high-tech tool, which uses the enzymes, receptors, antibodies, transport proteins, and other biological macromolecules as the stationary phase, has been used for screening active ingredients in traditional Chinese medicine and exploring new mechanisms of drug action.…”

Section: Introductionmentioning

confidence: 99%

An Escherichia coli Cell Membrane Chromatography-Offline LC-TOF-MS Method for Screening and Identifying Antimicrobial Peptides from Jatropha curcas Meal Protein Isolate Hydrolysates

Xiao

Zhang

2012

SLAS Discovery

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A novel, simple, and rapid method, named cell membrane affinity extraction (CMAE)-offline liquid chromatography timeof-flight mass spectrometry (LC-TOF-MS) was developed for screening and identifying antimicrobial peptides from Jatropha curcas meal protein isolate hydrolysates (JCMPIH) obtained by proteolytic enzyme (pepsin, trypsin, protamex, neutrase, flavourzyme, papain, alcalase, and acid protease) hydrolysis. A cationic antimicrobial peptide (CAILTHKR, JCpep8) was successfully isolated and identified by this method. Antimicrobial assay indicated that JCpep8 was active against the tested microorganisms (Escherichia coli ATCC 25922, Shigella dysenteriae ATCC 51302, Pseudomonas aeruginosa ATCC 27553, Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 23631, Streptococcus pneumoniae ATCC 49619) with minimal inhibitory concentration values ranging from 29 to 68 µg/mL. JCpep8 induced significant morphological alterations of the tested microbe surfaces, as shown by transmission electron microscopy, indicating strong membrane disruption. The results showed that CMAE-offline LC-TOF-MS could be a promising method for discovering high-throughput screening antimicrobial peptides from JCMPIH.

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Section: Introductionmentioning

confidence: 99%

An Escherichia coli Cell Membrane Chromatography-Offline LC-TOF-MS Method for Screening and Identifying Antimicrobial Peptides from Jatropha curcas Meal Protein Isolate Hydrolysates

Xiao

Zhang

2012

SLAS Discovery

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“…Cheese and milk products have been found to be a rich source of so-called bioactive peptides [36,75]. Depending on their functionality, bioactive peptides are divided into various groups, such as casomorphins, angiotensin-converting enzyme (ACE = dipeptidyl carboxypeptidase; EC 3.4.15.1)-inhibiting peptides, phosphopeptides, immunopeptides, casoplatelins (with antithrombotic effects) and antimicrobial and cytomodulating peptides [9,50].…”

Section: Introductionmentioning

confidence: 99%

ACE-inhibitory activity and ACE-inhibiting peptides in different cheese varieties

Sieber

Bütikofer

Egger

et al. 2009

Dairy Sci. Technol.

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-During the ripening of cheese, a large number of peptides are formed from casein. Some of these peptides have been shown to exert an antihypertensive effect due to their angiotensin-I-converting enzyme (ACE)-inhibitory activity. Recently, several studies have investigated the ACE-inhibiting potential of cheese, and various ACE-inhibiting peptides have been isolated and identified from different cheese varieties. The present review focuses on the occurrence of two tripeptides, Val-Pro-Pro and Ile-Pro-Pro, in cheese. These tripeptides were first described in fermented-milk products and have been demonstrated to exert a blood pressure-lowering effect in humans with mild hypertension. The influence of cheesemaking and ripening on the release of ACE-inhibiting peptides is revealed. Finally, the antihypertensive potential of cheese with high ACE-inhibitory activity is discussed with regard to the bioavailability of the peptides involved.

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“…It is the predominant caspase responsible for the cleavage of amyloidbeta 4A precursor protein which is linked with neuronal death in Alzheimer disease. Milk derived [28] peptides play vital roles in human health and nutrition. Numerous studies are dedicated to the casein fractions of the milk protein which accounts for 80% of milk total protein and is a rich source of bioactive peptides that stimulate and aid the immune system [29][30][31][32].…”

Section: Discussionmentioning

confidence: 99%

Identification of Bioactive Peptides in Goat Milk and Their Health Application

Sharma¹,

Rout²,

Kaushik³

et al. 2017

J Adv Dairy Res

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We have identified the bioactive peptides in goat milk using two dimensional gel electrophoresis and mass spectrometry. The milk samples were collected from three healthy breeds of goats at CIRG farms. The milk proteins were isolated using simple centrifugation and were evaluated on the basis of presence of other proteins variants using SDS PAGE and two dimensional gel electrophoresis. The protein profiles of all the milk proteins were same showing the presence of all the major caseins proteins and whey proteins whereas the two dimensional gel electrophoresis resulted gels has shown the presence of various protein spots. These protein spots were analysed under mass spectrometry and found biologically active peptides dipeptidyl peptidase, transcription factor A, Interleukin 12 subunit, oligodendrocyte transcription factor 2. In our study we have reported the biological significance of these peptides identified and their role in human health such as mitochondrial disease, brain malignancies, signaling processes of cytokines and help in generating cytotoxic lymphocyte helps enhance immunity.

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Antihypertensive and antimicrobial bioactive peptides from milk proteins

Cited by 157 publications

References 77 publications

An Escherichia coli Cell Membrane Chromatography-Offline LC-TOF-MS Method for Screening and Identifying Antimicrobial Peptides from Jatropha curcas Meal Protein Isolate Hydrolysates

An Escherichia coli Cell Membrane Chromatography-Offline LC-TOF-MS Method for Screening and Identifying Antimicrobial Peptides from Jatropha curcas Meal Protein Isolate Hydrolysates

ACE-inhibitory activity and ACE-inhibiting peptides in different cheese varieties

Identification of Bioactive Peptides in Goat Milk and Their Health Application

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