Antihyperglycemic Effect on Chronic Administration of Butanol Fraction of Ethanol Extract of Moringa Stenopetala Leaves in Alloxan Induced Diabetic Mice

Toma, Alemayehu; Makonnen, Eyasu; Debella, Asfaw; Tesfaye, Birhanu

doi:10.1016/s2221-1691(12)60461-4

Cited by 30 publications

(37 citation statements)

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“…The incredibly potent activity shown for the crude extract of M. stenopetala seeds when extracted under the method described herein also suggest that the plant could probably be developed as a cheap source of anticancer agent without even the need to isolate the active principle in its purest form. The identification of the isolated product as moringin (2) was based on comprehensive 2-D NMR studies including COSY, HMBC, HMQC as well as 1-D NMR ( 1 H, 13 C NMR and dept 13 C NMR analysis) experiments. The NMR data were generally in good agreement with those already published for 4(α-L-rhamnosyloxy)-benzyl isothiocyanate [37].…”

Section: Resultsmentioning

confidence: 99%

“…General analytical instrumentation and methodology: 1 H NMR, 13 C NMR and 2D-NMR (HMQC and HMBC) spectra were obtained on a JEOL 500 MHz instrument. Homonuclear 1 H connectivities were determined by using the COSY experiment.…”

Section: Isolation and Structural Assignments Of Compoundsmentioning

confidence: 99%

“…The various parts of M. stenopetala are extensively employed in the Ethiopian and Kenyan traditional medicine for treating a range of illnesses such as, diabetes, hypertension, stomach pain, malaria, leishmaniasis, leprosy, epilepsy, diarrhoea, asthma, colds, as an anthelmintic, emetic and wound healing [3]. Some of the recent pharmacological studies that appear to validate the claimed medicinal uses of the plant, primarily the leaves, include antimicrobial [4,5], antihypertensive [6][7][8], and blood glucose lowering and other favourable blood biochemical parameters [1,2,6,[9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

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Methodology for Rapid Isolation of Moringin: Potential Anticancer Compound from the Seeds of Moringa stenopetala

Habtemariam¹

2017

Pharm Anal Acta

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The seeds of Moringa stenopetala which are known to be rich sources of glucosinolates, primarily glucomoringin, have been shown to have limited anticancer effects when tested in vitro. In the present study, however, the water extract of the seeds obtained following defatting by hexane displayed potent cytotoxic activity against HepG2 (IC 50 , 6.28 ± 0.55 µg/ml) and the SH-SY5Y human neuroblastoma (IC 50 , 9.81 ± 1.30 µg/ml) cells. The methanol extract displayed a weak cytotoxic activity against the HepG2 human hepatocellular cancer cells when tested upto 500 µg/ml, while extraction with hexane yielded the non-cytotoxic fixed oil which fatty acid composition was primarily oleic acid (75%). By monitoring the constituents of the water extract by HPLC and a one-step combiflash chromatographic system using C-18 silica gel system, the principal active constituent (glucomoringin isothiocyanate or moringin; 4(α-L-rhamnosyloxy)-benzyl isothiocyanate) was isolated. Moringin was 6.3 and 2.4 times more potent than etoposide in the HepG2 and SH-Sy5Y cell lines respectively. The isolation of this potent potential anticancer compound from the seeds of M. stenopetala without the need for multi-step chromatographic and enzyme-digestionbased purification steps are discussed.

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Section: Resultsmentioning

confidence: 99%

Section: Isolation and Structural Assignments Of Compoundsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Methodology for Rapid Isolation of Moringin: Potential Anticancer Compound from the Seeds of Moringa stenopetala

Habtemariam¹

2017

Pharm Anal Acta

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“…They indicated the need for further studies in order to fractionate the active Science Target Inc. www.sciencetarget.com principle and find out the mechanism(s) of action of M. stenopetala leaf extract on blood glucose and cholesterol levels in animal models to ascertain its therapeutic importance. Similarly, experiments conducted on animal models showed that butanol fraction of the ethanol extract of M. stenopetala leaves has anti-hyperglycemic and anti-hyperlipedemic effects, and as a result, can be used to treat diabetes (Toma et al, 2012). These researchers indicated that administration of butanol fraction of ethanol extract of M. stenopetala leaves to diabetic mice resulted in significant reduction of fasting blood glucose level, serum total cholesterol, and triglyceride levels.…”

Section: Antimicrobial and Medicinal Valuesmentioning

confidence: 96%

Actual and Potential Applications of Moringa stenopetala, Underutilized Indigenous Vegetable of Southern Ethiopia: A Review

Seifu¹

2015

International Journal of Agricultural and Food Research

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Moringa stenopetala, locally called Haleko, is an indigenous vegetable tree native to southern Ethiopia and grown mainly for its food value. It is a drought tolerant and fast growing tree that has high nutritional value and the potential to alleviate malnutrition in the tropics. Millions of people in southern Ethiopia consume its leaves as a staple vegetable. It also has potential medicinal and industrial applications and significant economic contributions. Despite its actual and potential roles, M. stenopetala has been given little research and development attention. This paper reports actual and potential uses of Moringa stenopetala and highlights research gaps that need to be addressed in the future.

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“…It has been reported that, M. stenopetala (Baker f.) Cufod. has hypotensive (Mengistu et al, 2012), antihyperglycemic (Toma et al, 2012, 2015; Sileshi et al, 2014) and also has a nutritional value (Abuye et al, 2004). The objective of the present study is, therefore, to investigate the antihypertensive and antihyperlipidemic effects of extracts and fractions of M. stenopetala (Baker f.) Cufod.…”

Section: Introductionmentioning

confidence: 99%

In vivo Antihypertensive and Antihyperlipidemic Effects of the Crude Extracts and Fractions of Moringa stenopetala (Baker f.) Cufod. Leaves in Rats

et al. 2016

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Background: Moringa stenopetala (Baker f.) Cufod. is a medicinal plant that has been used in Ethiopian traditional medicine as a remedy for treatment of hypertension and diabetes. The aim of this study was to evaluate antihypertensive and antihyperlipidemic effect in fructose induced hypertensive rats.Methods: Rats were randomly divided into control and treatment groups (n = 6). Treatment groups were given daily extracts (250, 500, and 1000 mg/kg) orally with fructose. Whereas, positive, negative and normal control groups were received captopril (20 mg/kg/day with fructose), only fructose (66% w/v ad libitum) and distilled water ad libitum for 15 days, respectively. The blood pressure was measured every 5th day using tail cuff blood pressure analyzer, and on the 16th day the blood was sampled to evaluate antihyperlipidemic effect using clinical chemistry analyzer.Results: The study showed that aqueous and 70% ethanol extracts significantly prevented blood pressure increment in a dose dependent manner comparable to that of the standard drug. Similarly, the extracts suppressed increment in lipid profile (cholesterol, glucose, and triglycerides) compared with negative control. The biochemical test revealed that extracts produced a rise in liver but no effect on kidney function indicators compared with normal control.Conclusion: These findings revealed that both crude extracts of M. stenopetala (Baker f.) Cufod. possess antihypertensive and antihyperlipidemic effect.

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Antihyperglycemic Effect on Chronic Administration of Butanol Fraction of Ethanol Extract of Moringa Stenopetala Leaves in Alloxan Induced Diabetic Mice

Cited by 30 publications

References 6 publications

Methodology for Rapid Isolation of Moringin: Potential Anticancer Compound from the Seeds of Moringa stenopetala

Methodology for Rapid Isolation of Moringin: Potential Anticancer Compound from the Seeds of Moringa stenopetala

Actual and Potential Applications of Moringa stenopetala, Underutilized Indigenous Vegetable of Southern Ethiopia: A Review

In vivo Antihypertensive and Antihyperlipidemic Effects of the Crude Extracts and Fractions of Moringa stenopetala (Baker f.) Cufod. Leaves in Rats

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