Antihyperglycemic and antihyperlipidemic properties of aqueous root extract of Icacina senegalensis in alloxan induced diabetic rats

Akuodor, Godwin Christian; Udia, P. M.; Bassey, Augustine Ini Lawrence; Chilaka, Kingsley Chimsorom; Okezie, OA

doi:10.1016/s2221-6189(14)60025-1

Cited by 15 publications

(13 citation statements)

References 20 publications

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“…These free radicals dismutate to hydrogen peroxide with a simultaneous flooding of cytosolic calcium ions, which cause speedy destruction of pancreatic beta cells of the islets of langerhans. This is sequel to a significant decrease in endogenous insulin secretion and the resultant increase in the blood glucose levels (Akuodor et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

“…The pancreatic islet cells of the diabetic rats treated with 2 mg/kg glibenclamide showed good population, indicating that the alloxan-induced lesion in this group of rats was mild. The destructive effects of alloxan on the pancreatic cells have been widely reported (Szukudelski, 2001;Lenzen, 2008;Akuodor et al, 2014) but still need to be better understood.…”

Section: Resultsmentioning

confidence: 99%

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Ameliorative Effects of <i>Cussonia arborea</i> Methanol Root Bark Extract on Histomorphology of Pancreas of Alloxan-Induced Diabetic Rats

Patrick

Asuzu

2016

Not Sci Biol

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Diabetes mellitus is an endocrine and a metabolic disease resulting from the destruction of pancreatic beta cells; thus assessment of the pancreas in diabetic rats is important in monitoring therapy. The hereby study assessed pancreatic status of diabetic rats treated with methanol root bark extract of Cussonia arborea. A total of seventy two (72) male albino wistar rats weighting between 100-105 g were assigned into six (6) groups of twelve (12) rats per group. Groups 1-5 were diabetic infected by single intraperitoneal injection with alloxan monohydrate, at the dose of 160 mg/kg and treated with 62.5, 125, 250 mg/kg bw of the extract, 2 mg/kg bw glibenclamide and 10 ml/kg distilled water (DW) respectively, while the non diabetic rats, represented by Group 6, received 10 ml/kg DW and served as normal control rats. The treatment was applied daily through the oral route for 84 days. At the end of the experiment, the pancreas organs were acquired under light ether anaesthesia for histomorphometric assessment. The results indicated that the cells of the islet of langerhans of the diabetic untreated rats (Group 5) were severely depleted when compared to that of the normal rats (Group 6). The islet cells of the diabetic rats treated with 125 mg/kg Cussonia arborea extract (Group 2) was comparable to that of the diabetic rats treated with glibenclamide (Group 4) and the normal control rats. It was concluded that the methanol extract of C. arborea, especially at the dose of 125 mg/kg, ameliorated pancreatic lesions induced by diabetes occasioned by alloxan.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Ameliorative Effects of <i>Cussonia arborea</i> Methanol Root Bark Extract on Histomorphology of Pancreas of Alloxan-Induced Diabetic Rats

Patrick

Asuzu

2016

Not Sci Biol

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“…Among them, Yanarday and Colak [16] who find that injection of a single dose of (150mg/kg) body weight of alloxan after 18hrs fasting induced hyperglycemia in experimental rats. Gosh and Suryawanshi [9]; Akuodor, et al [11] were also reported that (150mg/kg) of alloxan monohydrate induced diabetes in rats. However, other workers reported different results in which different doses were needed to induce diabetes even though a single dose was still capable of inducing hyperglycemia.…”

Section: Discussionmentioning

confidence: 99%

“…Glycemic control is important to avoid these complications [5,6].The experimental animal model of a disease is important to understand the disease pathophysiology and also the development of drugs used for it as treatment [7]. Various diabetic animal models have been established either surgically [8] orchemically [7] using variety of modes, doses and routes of administration [9][10][11]. Alloxan in its monohydrate form is one of the chemical agents used for the diabetes induction since long [12,13].…”

Section: Introductionmentioning

confidence: 99%

Alloxan Dose Optimization to Induce Diabetes in Albino Mice and the Determination of the Induced Diabetes Type

Oshkondali¹,

Mahmoudy²,

Samira³

et al. 2019

SJMPS

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Several chemical compounds are used worldwide to induce diabetes in animals. Alloxan monohydrate and streptozotocin are among those are commonly used because of their cheap coast. However, other chemical compounds are less toxic but more expensive. This pilot study was conducted to use alloxan as diabetes inducer, and to determine the suitable dose which can make a diabetic albino mice model. The type of induced diabetes was also differentiated by using glimepiride tablets. Three mice were injected with three doses of alloxan monohydrate intraperitoneally (150mg-100mg-150mg)/kg in two days intervals respectively in order to induce and maintain diabetes. The fasting blood sugar measured before and after each alloxan injection. Results showed an increasing in blood glucose levels from an average of 81 mg/dl to 168.6 mg/dl after the first dose (150 mg /kg) of alloxan, after 2nd dose (100 mg /kg) of alloxan from 117.83 mg/dl to 154.67 mg/dl, and from 154.67 mg/dl to 173 mg/dl after 3rd dose (150 mg/kg). On the other hand the glimepiride which was given to mice orally caused dramatic decrease in blood sugar level from 173 mg/dl to 93.67 mg/dl after 4 hrs of administration. From this study it was conducted that the use of different doses of alloxan can induce and maintain diabetes and the induced diabetes is type II. Further work is in progress to examine the use of plant extracts in order to regulate blood sugar levels in induced diabetic mice.

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“…The results suggest that insulin resistance and hyperinsulinemia are not induced in the excessive weight gain observed in their animal model. The decline in the level of insulin reflect difficult in the secretion of insulin because of degeneration of Langerhans cell indicates induction of type II model as indicated in the histopathology of pancreas 18,19,20,21 . Other factors which may contribute to the increased in obesity and diabetes are altered of lipid profile.…”

Section: Hfd Compositionmentioning

confidence: 99%

Untitled

2019

IJPSR

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Aim: The present investigation was carried out by evaluating the effect of protocatechuic acid (PCA) on a high-fat diet (HFD) along with a low dose of alloxan-induced hyperglycemia in male Wistar rats. Methods: In the present study, administration of high fatty diet (HFD) for 45 days along with low dose of alloxan (80 mg/kg) in rats on 30 days produced significant high fatty diet in the, body weight, low density lipoproteins (LDL), triglycerides (TG) and blood glucose levels while decreases the high density lipoprotein (HDL) and serum insulin level. Protocatechuic acid (PCA) was started to administered at a dose of 50, 100, 200 mg/kg (orally) on day 45 of HFD administration and continue further 45 days. Glibenclamide was used as reference standard.Results: Results of PCA shows a significant decreased in body weight, blood glucose level after 45 days of treatment in diabetic animals. Result also indicated to increase in serum insulin level. The result of lipid profile indicated to normalize after 45 days treatment with PCA in diabetic rats. All the results are compared with reference standard glibenclamide. Conclusion: From this result, it concludes that PCA decreases the body weight, blood glucose level dose-dependently indicated antidiabetic and antiobesity activity. Antidiabetic activity of PCA is associated with increased in the level of serum insulin.

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Antihyperglycemic and antihyperlipidemic properties of aqueous root extract of Icacina senegalensis in alloxan induced diabetic rats

Cited by 15 publications

References 20 publications

Ameliorative Effects of <i>Cussonia arborea</i> Methanol Root Bark Extract on Histomorphology of Pancreas of Alloxan-Induced Diabetic Rats

Ameliorative Effects of <i>Cussonia arborea</i> Methanol Root Bark Extract on Histomorphology of Pancreas of Alloxan-Induced Diabetic Rats

Alloxan Dose Optimization to Induce Diabetes in Albino Mice and the Determination of the Induced Diabetes Type

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