Antihyperglycaemic activity of the methanol extract from leaves of <i>Eremophila maculata</i> (Scrophulariaceae) in streptozotocin-induced diabetic rats

Youssef, Fadia S.; Ashour, Mohamed L.; Ebada, Sherif S.; Sobeh, Mansour; El-Beshbishy, Hesham A.; Singab, Abdel Nasser B.

doi:10.1111/jphp.12690

Cited by 33 publications

(40 citation statements)

References 26 publications

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“…This was done by applying both pH and rule-based ionization methods for the preparation of ligands with Discovery Studio 2.5 (Accelrys Inc., San Diego, CA, United States). In silico virtual screening was performed within the active pocket of the previously mentioned enzymes and the free binding energies for the highly stable docking poses were calculated as previously discussed ( Sobeh et al, 2016 ; Ashour et al, 2017 ; Youssef et al, 2017 ).…”

Section: Methodsmentioning

confidence: 99%

Syzygium aqueum: A Polyphenol- Rich Leaf Extract Exhibits Antioxidant, Hepatoprotective, Pain-Killing and Anti-inflammatory Activities in Animal Models

et al. 2018

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Syzygium aqueum is widely used in folk medicine. A polyphenol-rich extract from its leaves demonstrated a plethora of substantial pharmacological properties. The extract showed solid antioxidant properties in vitro and protected human keratinocytes (HaCaT cells) against UVA damage. The extract also reduced the elevated levels of ALT, AST, total bilirubin (TB), total cholesterol (TC) and triglycerides (TG) in rats with acute CCl4 intoxication. In addition to reducing the high MDA level, the extract noticeably restored GSH and SOD to the normal control levels in liver tissue homogenates and counteracted the deleterious histopathologic changes in liver after CCl4 injection. Additionally, the extract exhibited promising anti-inflammatory activities in vitro where it inhibited LOX, COX-1, and COX-2 with a higher COX-2 selectivity than that of indomethacin and diclofenac and reduced the extent of lysis of erythrocytes upon incubation with hypotonic buffer solution. S. aqueum extract also markedly reduced leukocyte numbers with similar activities to diclofenac in rats challenged with carrageenan. Additionally, administration of the extract abolished writhes induced by acetic acid in mice and prolonged the response latency in hot plate test. Meanwhile, the identified polyphenolics from the extract showed a certain affinity for the active pockets of 5-lipoxygenase (5-LOX), cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) explaining the observed anti-inflammatory activities. Finally, 87 secondary metabolites (mostly phenolics) were tentatively identified in the extract based on LC-MS/MS analyses. Syzygium aqueum displays good protection against oxidative stress, free radicals, and could be a good candidate for treating oxidative stress related diseases.

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Section: Methodsmentioning

confidence: 99%

Syzygium aqueum: A Polyphenol- Rich Leaf Extract Exhibits Antioxidant, Hepatoprotective, Pain-Killing and Anti-inflammatory Activities in Animal Models

et al. 2018

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“…The structure of the enzyme was established using the default protein preparation protocol of Accelry’s discovery studio 2.5 (Accelrys ® , Inc., San Diego, CA, USA). Molecular modelling studies were done using Accelry’s discovery studio 2.5 (Accelrys ® , Inc., San Diego, CA, USA) according to what was previously reported [ 30 , 31 , 32 ] and the binding free energies were calculated applying the following equation: ΔG binding = E complex − (E GR + E ligand ) where ΔG binding The ligand–enzyme interaction binding energy E complex The potential energy for the complex of GR bound with the ligand E GR The potential energy of the protein alone E ligand The potential energy for the ligand alone

…”

Section: Methodsmentioning

confidence: 99%

Chemical Composition of Pinus roxburghii Bark Volatile Oil and Validation of Its Anti-Inflammatory Activity Using Molecular Modelling and Bleomycin-Induced Inflammation in Albino Mice

et al. 2017

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The chemical composition of Pinus roxburghii bark essential oil (PRO) was qualitatively and quantitatively determined using GC/FID and GC/MS. The anti-inflammatory activity was assessed in vitro by evaluating the binding percentages on the cannabinoids and opioids receptors. Bleomycin (BLM)-induced pulmonary inflammation in albino mice was adopted to assess PRO anti-inflammatory efficacy in vivo. In silico molecular modelling of its major components was performed on human glucocorticoids receptor (GR). Seventy-five components were identified in which longifolene (33.13%) and palmitic acid (9.34%) constituted the predominant components. No binding was observed on cannabinoid receptor type 1 (CB1), whereas mild binding was observed on cannabinoid receptor type 2 (CB2), delta, kappa, and mu receptors accounting for 2.9%, 6.9%, 10.9% and 22% binding. A significant in vivo activity was evidenced by reduction of the elevated malondialdehyde (MDA), nitric oxide (NO), myeloperoxidase (MPO), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) levels by 55.56%, 55.66%, 64.64%, 58.85% and 77.78% with concomitant elevation of superoxide dismutase (SOD) and catalase (CAT) activities comparable to BLM-treated group at 100 mg/kg body weight. In silico studies showed that palmitic acid exerted the fittest binding. PRO could serve as a potent anti-inflammatory natural candidate that should be supported by further clinical trials.

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“…The α-amylase enzyme is critical in assisting in the catalysis of the first steps in the conversion of starch into maltose, and subsequently to glucose [22,23]. Nowadays, α-amylase inhibitors are used in therapeutic approaches to counteract hyperglycemia.…”

Section: Antioxidant Potential Of Different Ferula Speciesmentioning

confidence: 99%

Chemical Profiling and Discrimination of Essential Oils from Six Ferula Species Using GC Analyses Coupled with Chemometrics and Evaluation of Their Antioxidant and Enzyme Inhibitory Potential

et al. 2020

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The differences in the composition of essential oils obtained from the aerial parts of six Ferula species viz., F. caratavica (Fc), F. kuchistanica (Fk), F. pseudoreoselinum (Fp), F. samarcandica (Fs), F. tenuisecta (Ft) and F. varia (Fv) were detected both qualitatively and quantitatively using GC-MS and GC-FID analyses. One hundred and six metabolites were identified that account for 92.1, 96.43, 87.43, 95.95, 92.90 and 89.48% of Fc, Fk, Fp, Fs, Ft and Fv whole essential oils, respectively. The data from the GC-MS analyses were subjected to unsupervised pattern recognition chemometric analysis utilizing principal component analysis (PCA) to improve the visualization of such differences among the six species. Fk and Ft are very closely related to each other and were gathered together in one cluster. The antioxidant potential was assessed in vitro using different assays including 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), cupric reducing antioxidant capacity (CUPRAC), ferric reducing power (FRAP) and phosphomolybdenum (PM) assays. Ft and Fp exhibited the most notable antioxidant properties as evidenced by their pronounced activities in most of the antioxidant assays performed, followed by Fc. Fk showed the most effective tyrosinase inhibitory potential, which was estimated as 119.67 mgKAE/g oil, while Fp exhibited the most potent α-amylase inhibitory potential, which was equivalent to 2.61 mmol ACAE/g oil. Thus, it was concluded that Ferula species could serve as a promising natural antioxidant drug that could be included in different products and spices to alleviate hyperglycemia and used as a natural ingredient in pharmaceutical cosmetics to counteract hyperpigmentation.

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Antihyperglycaemic activity of the methanol extract from leaves of Eremophila maculata (Scrophulariaceae) in streptozotocin-induced diabetic rats

Abstract: Eremophila maculata offers an interesting relatively safer antihyperglycaemic candidate comparable to synthetic analogues.

Cited by 33 publications

References 26 publications

Syzygium aqueum: A Polyphenol- Rich Leaf Extract Exhibits Antioxidant, Hepatoprotective, Pain-Killing and Anti-inflammatory Activities in Animal Models

Syzygium aqueum: A Polyphenol- Rich Leaf Extract Exhibits Antioxidant, Hepatoprotective, Pain-Killing and Anti-inflammatory Activities in Animal Models

Chemical Composition of Pinus roxburghii Bark Volatile Oil and Validation of Its Anti-Inflammatory Activity Using Molecular Modelling and Bleomycin-Induced Inflammation in Albino Mice

Chemical Profiling and Discrimination of Essential Oils from Six Ferula Species Using GC Analyses Coupled with Chemometrics and Evaluation of Their Antioxidant and Enzyme Inhibitory Potential

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