Antihypercholesterolemic and antioxidative effects of an extract of the oyster mushroom, Pleurotus ostreatus, and its major constituent, chrysin, in Triton WR-1339-induced hypercholesterolemic rats

Abstract

Hypercholesterolemia and oxidative stress are known to accelerate coronary artery disease and progression of atherosclerotic lesions. In the present study, an attempt was made to evaluate the putative antihypercholesterolemic and antioxidative effects of an ethanolic extract of the oyster mushroom (Pleurotus ostreatus) and chrysin, one of its major components, in hypercholesterolemic rats. Hypercholesterolemia was induced in rats by a single intraperitoneal injection of Triton WR-1339 (300 mg/kg body weight (b.wt.)), which resulted in persistently elevated blood/serum levels of glucose, lipid profile parameters (total cholesterol, triglycerides, low-density lipoprotein-, and very low-density lipoprotein-cholesterol), and of hepatic marker enzymes (alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and lactate dehydrogenase). In addition, lowered mean activities of hepatic antioxidant enzymes (catalase, superoxide dismutase, and glutathione peroxidase) and lowered mean levels of nonenzymatic antioxidants (reduced glutathione, vitamin C, and vitamin E) were observed. Oral administration of the mushroom extract (500 mg/kg b.wt.) and chrysin (200 mg/kg b.wt.) to hypercholesterolemic rats for 7 days resulted in a significant decrease in mean blood/serum levels of glucose, lipid profile parameters, and hepatic marker enzymes and a concomitant increase in enzymatic and nonenzymatic antioxidant parameters. The hypercholesterolemia-ameliorating effect was more pronounced in chrysin-treated rats than in extract-treated rats, being almost as effective as that of the standard lipid-lowering drug, lovastatin (10 mg/kg b.wt.). These results suggest that chrysin, a major component of the oyster mushroom extract, may protect against the hypercholesterolemia and elevated serum hepatic marker enzyme levels induced in rats injected with Triton WR-1339.

Hypolipidemic effect of aqueous leaf extract of carmona microphylla G Don

“…It is widely used, particularly in mice, for screening natural or chemical hypolipidemic drugs [16,[19][20]. In the present study, TC and TG levels were largely elevated 24 h after the triton WR-1339 administration, which was significantly decreased by the treatment with CAE.…”

Antihypercholesterolemic Effects of Mushroom, Chrysin, Curcumin and Omega-3 in Experimental Hypercholesterolemic Rats

“…1). The solvent was evaporated under reduced pressure at 45°C by using a rotary evaporator for elimination of ethanol, and the dried extract was stored at 4 ∘ C until further use [27].…”

“…Mushroom extract administered group (500 mg/kg b.wt. / day) where mushroom was given orally for 14 days [27]. Chrysin administered rats in a dose of 200 mg/kg b.wt.…”

“…Chrysin administered rats in a dose of 200 mg/kg b.wt. / day in 0.5 % dimethyl sulfoxide (DMSO) orally for 14 days [27]. Curcumin administered rats in a dose of 400 mg/kg b.wt.…”

A New Validated Simultaneous Reversed-Phase High-Performance Liquid Chromatography Assay Method for Estimation of Two Flavones (Baicalein and Chrysin) in Api Drugs

“…Baicalein inhibits the action potential of the neurons that raise anxiety. In mice, baicalein produces anxietylowering effects without causing drowsiness or muscle relaxation [1][2]. Baicalein was shown in Figs.…”