Antihyaluronidase and Antioxidant Potential of Atriplex sagittata Borkh. in Relation to Phenolic Compounds and Triterpene Saponins

Abstract

The genus Atriplex provides species that are used as food and natural remedies. In this work, the levels of soluble phenolic acids (free and conjugated) and flavonoids in extracts from roots, stems, leaves and flowers of the unexplored Atriplex sagittata Borkh were investigated by LC-ESI-MS/MS, together with their antioxidant and antihyaluronidase activity. Phenolic acids were present in all parts of A. sagittata; and were most abundant in the leaves (225.24 μg/g dw.), whereas the highest content of flavonoids were found in the flowers (242.71 μg/g dw.). The most common phenolics were 4-hydroxybenzoic and salicylic acids, kaempferol-3-glucoside-7-rhamnoside, kaempferol-3-rutinoside and the rare narcissoside, which was present in almost all morphotic parts. The stem extract had the highest antioxidant activity and total phenolic content (611.86 mg/100 g dw.), whereas flower extract exerted the most potent antihyaluronidase effect (IC50 = 84.67 µg/mL; control—quercetin: IC50 = 514.28 μg/mL). Phytochemical analysis of the flower extract led to the isolation of two triterpene saponins that were shown to be strong hyaluronidase inhibitors (IC50 = 33.77 and 168.15 µg/mL; control—escin: IC50 = 307.38 µg/mL). This is the first report on the presence of phenolics and saponins in A. sagittata. The results suggest that both groups of metabolites may contribute to the overall activity of this plant species.

Peptides of Yeast Saccharomyces cerevisiae Activated by the Aquatic Extract of Atriplex sibirica L.

“…halimus L., Atriplex nummularia, Atriplex laciniata L., and A. sagittata Borkh. ,,,, In the present study, Atriplex sibirica L. is selected as a source of biologically active substances, and the possibility of using A. sibirica L. as a source of additional nutrients for the activation of reproduction of S.…”

“…The antihyaluronidase and antioxidant potential of Atriplex sagittata Borkh toward phenolic compounds and triterpene saponins has been highlighted. 31 with the chemical composition, antibacterial and antioxidant activity has also been established. 32 Safety of use and absence of toxicity to living organisms of the substances released from Atriplex L. have been proved in numerous studies.…”

“…The basic information on the biological activity of the compounds has been reported for the orach species Atriplex fera, A. halimus L., Atriplex nummularia, Atriplex laciniata L., and A. sagittata Borkh. 18,22,26,27,31 In the present study, Atriplex sibirica L. is selected as a source of biologically active substances, and the possibility of using A. sibirica L. as a source of additional nutrients for the activation of reproduction of S. cerevisiae and biosynthesis of nitrogenous substances, biologically active peptides, is considered. This study suggests and explores the use of A. sibirica L. for intensification of nitrogenous substances biosynthesis and analyzes the potential biological activity of peptides extracted from yeast trypsin hydrolysates.…”

“…Several studies have identified antidiabetic, anabolic, antihyperglycemic, and antihyperlipidemic properties of Atriplex L. plants. The antihyaluronidase and antioxidant potential of Atriplex sagittata Borkh toward phenolic compounds and triterpene saponins has been highlighted . For essential oils of Atriplex semibaccata, along with the chemical composition, antibacterial and antioxidant activity has also been established …”

Phytochemical Analysis, Acetylcholinesterase Inhibition, Antidiabetic and Antioxidant Activities of Atriplex halimus L. (Amaranthaceae Juss.)

“…As well as cardiovascular disease, it is also used in the treatment of anemia trouble and rheumatism. [10] Recent studies reported antioxidant, [15][16][17][18] Antiinflammatory, [19] Antimicrobial [20,21] , hypoglycemic, [22,23] Hepatoprotective, [24] anti-Alzheimer, [25,26] antidiabetic [9,27] and Wound healing [15] properties of plant extracts and phytochemicals from Mediterranean saltbush.…”