Antigens with application toward immunecontrol of blood-feeding parasitic nematodes

Jasmer, Douglas P.; McGuire, Travis C.

doi:10.1016/s0007-1935(96)80099-6

Cited by 11 publications

(5 citation statements)

References 69 publications

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“…Due to the emergence of drug resistance, many researchers have been focusing on ES molecules as protective antigens from the gut of the parasite or so called 'hidden antigens' and their immunogenic nature serve as an alternative source of protection against helminths infection and/or disease [10][11][12][13][14]. In previous studies, two low molecular weight antigens from H. contortus, ES-15, and its closely related ES-24 were purified from ES products and determined for their protective potential against parasite infection in vivo [1,15].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Haemonchus contortus Excretory/Secretory Antigen (ES-15) and Its Modulatory Functions on Goat Immune Cells In Vitro

et al. 2020

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Small size excretory/secretory (ES) antigens of the Haemonchus contortus parasite have intense interest among researchers for understanding the molecular basis of helminths immune regulation in term of control strategies. Immunomodulatory roles of H. contortus ES-15 kDa (HcES-15) on host immune cells during host–parasite interactions are unknown. In this study, the HcES-15 gene was cloned and expression of recombinant protein (rHcES-15) was induced by isopropyl-ß-d-thiogalactopyranoside (IPTG). Binding activity of rHcES-15 to goat peripheral blood mononuclear cells (PBMCs) was confirmed by immunofluorescence assay (IFA) and immunohistochemical analysis showed that H. contortus 15 kDa protein localized in the outer and inner structure of the adult worm, clearly indicated as the parasite’s ES antigen. The immunoregulatory role on cytokines production, cell proliferation, cell migration, nitric oxide (NO) production, apoptosis, and phagocytosis were observed by co-incubation of rHcES-15 with goat PBMCs. The results showed that cytokines IL-4, IL-10, IL-17, the production of nitric oxide (NO), PBMCs apoptosis, and monocytes phagocytosis were all elevated after cells incubated with rHcES-15 at differential protein concentrations. We also found that IFN-γ, TGF-β1, cells proliferation and migration were significantly suppressed with the interaction of rHcES-15 protein. Our findings indicated that low molecular ES antigens of H. contortus possessed discrete immunoregulatory roles, which will help to understand the mechanisms involved in immune evasion by the parasite during host–parasite interactions.

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Section: Introductionmentioning

confidence: 99%

Characterization of Haemonchus contortus Excretory/Secretory Antigen (ES-15) and Its Modulatory Functions on Goat Immune Cells In Vitro

et al. 2020

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“…Taxas de Redução na Contagem de Ovos (RCOF) nas fezes dos ovinos do G1 -controle não tratado, G2 -tratados com larvas inativadas, G3 -tratados com larvas inativadas + alho in natura e G4 tratados apenas com alho in natura, de acordo com os valores dos OPGs ao final ao experimento (dia 144). McGUIRE, 1996). Neste estudo, todavia, a grande variedade e a pouca quantidade de antígenos imunogênicos presentes nas larvas inativadas podem ter colaborado com a baixa eficácia da imunização se comparado à induzida por antígenos purificados (H11, H-gal-GP por exemplo).…”

Section: Resultsunclassified

“…Dentre os métodos de controle bioterápicos, a vacinação vem apresentando resultados promissores a partir da utilização de antígenos de helmintos de ovinos (JASMER;McGUIRE,1996). Antígenos naturais foram identificados a partir de larvas de 3º e 4º estágio (NEWTON et al, 1995) e também de adultos (CUQUERELLA et al, 1994).…”

Section: Introductionunclassified

Fito-bioterapia no controle de nematódeos gastrointestinais de ovinos

et al. 2013

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ResumoA resistência parasitária e a tendência dos últimos anos de mudança para sistemas orgânicos tem resultado em diversas alternativas de manejo animal como a seleção de raças resistentes, integração com outras espécies animais, fitoterapia, fungos nematófagos, vacinas contra nematódeos, entre outros. Visando uma alternativa para controle da verminose ovina, objetivou-se analisar o efeito da administração de larvas inativadas de Haemonchus associadas ou não a alho in natura no controle de nematódeos gastrointestinais em ovinos naturalmente infectados. Para tanto, 32 ovinos foram avaliados por até 144 dias após tratamentos com larvas inativadas de Haemonchus e/ou alho in natura, quanto à diminuição da eliminação de ovos nas fezes (taxa de Redução na Contagem de Ovos nas Fezes -RCOF), eficiência destes tratamentos sob os gêneros de helmintos e alterações hematológicas. Observou-se aumento significativo na contagem de OPG dos grupos G1 (controle) e G4 (alho in natura). Ao final do experimento observou-se menor contagem de OPG no G3 (larvas inativadas + alho in natura) em relação ao G1, mas sem diferença significativa entre o G3 e os outros tratamentos. Quanto a RCOF, verificou-se redução média de 44% na contagem de ovos do grupo G2 (larvas inativadas) e do grupo G3 e um aumento de 44% no grupo G4. A eficácia dos tratamentos sob o gênero Haemonchus variou de 0 a 77%, sendo esta ultima taxa observada 67 dias no G3, justamente 7 dias depois de observado aumento significativo de linfócitos. Concluiu-se neste estudo que o alho in natura administrado na concentração de 2,5mg/Kg não foi capaz de reduzir a carga parasitária dos ovinos, mas que, quando associado a administração de larvas inativadas de Haemonchus pode ter contribuído como imunoestimulante e que a redução dos ovos eliminados nas fezes dos ovinos tratados com larvas inativadas, apesar de insuficiente para ser considerado um método de controle eficaz, demonstra a possibilidade de viabilidade no uso de vacinas contra Haemonchose. Palavras-chave: Fitoterapia, bioterapia, verminose, pequenos ruminantes AbstractThe parasitic resistance and the tendency of recent years to shift to organic systems has resulted in several alternative animal management as the selection of resistant breeds, integration with other animal species, phytotherapy, nematophagous fungi, nematodes vaccines, among others. Looking for an alternative control of nematode parasites of sheep, this paper aimed to analyze the effect of administration of inactivated larvae of Haemonchus and fresh garlic on the level infection with gastrointestinal nematodes in naturally infected sheep. Therefore, 32 animals were monitored for 144 days after phyto-biotherapy treatments in relation to reduction of parasite eggs (egg reduction rate -FECR), these treatments under the genres of helminths and hematological changes. It was observed significant increase in the EPGs of G1 (control group) and G4 (fresh garlic). At the end of the experiment observed lower EPG in G3 (inactivated larvae + fresh garlic) compared to...

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“…One animal of UV 60 min group was slaughtered at 30 day post vaccination and its G.I.T (omasum, abomasums) did not contain any worms. The irradiation of infective larvae inhibited its further development to fourth and adult life cycle stages after infection, thereby providing prolonged antigen stimulation under conditions that largely obviate clinical disease ( 18 ). So exposure of H. contortus L3 for one hour to Ultraviolet rays is sufficient for attenuation.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, normal eggs appeared only in UV30 min group on 18 day post immunization and before challange. This may be meant the exposure of H. contortus L3 for a half hour to Ultraviolet rays is insufficient for attenuation ( 18 ).…”

Section: Discussionmentioning

confidence: 99%

Attenuation effect of UV on Haemonchus contortus Larvae in Experimentally Infected Goats

Aboelhadid

El-Askalany

Mousa

et al. 2013

Journal of Veterinary Medical Research

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This study was conducted to study the UV protective effect against Haemonchus contortus infection in goats. Sixteen male goats were divided into 5 groups, control infected, control uninfected and UV 30minutes; UV 60minutes and UV booster 60minutes exposure. The UV groups were exposed to UV irradiation at wave length 254nm for 30 and 60 minutes. The UV booster 60min was administrated 2 doses of exposed larvae with an interval of one month. All groups except the control negative one were challenged for 42 days from the beginning. In UV booster 60min had reduction in egg count per gram feces and worm burden (93% & 34 % respectively). These parameters were similar in control infected, UV 30min and UV 60min groups. Increases in levels of antibodies were found in goats of UV booster 60min group the other groups. Finally, 2 doses of UV 60min exposure could protect goats from H. contortus. Material and methodsHaemonchus contortus larvae. H. contortus adult worms were obtained from our local abattoir in Beni-Suef city, Egypt. The adult worms were collected from the omasum, and from abomasum of infected abattoir's sheep and were washed several times with phosphate buffer

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Antigens with application toward immunecontrol of blood-feeding parasitic nematodes

Cited by 11 publications

References 69 publications

Characterization of Haemonchus contortus Excretory/Secretory Antigen (ES-15) and Its Modulatory Functions on Goat Immune Cells In Vitro

Characterization of Haemonchus contortus Excretory/Secretory Antigen (ES-15) and Its Modulatory Functions on Goat Immune Cells In Vitro

Fito-bioterapia no controle de nematódeos gastrointestinais de ovinos

Attenuation effect of UV on Haemonchus contortus Larvae in Experimentally Infected Goats

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