Antigens ofEdwardsiella ictalurirecognised by serum antibodies from naturally infected channel catfish

Baldwin, Thomas J.; Collins, L A; Newton, Joseph C.

doi:10.1006/fsim.1997.0081

Cited by 18 publications

(23 citation statements)

References 11 publications

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“…LPS has been extensively studied in E. ictaluri and has been implicated in the pathogenesis primarily through its ability to elicit a strong antibody response in catfish [18,19,[34][35][36]. LPS was first characterised by Nomura and Aoki [34] as four or five bands occurring in the low mass region of 15% acrylamide gels.…”

Section: Discussionmentioning

confidence: 99%

“…These OMPs have been shown to contain several important bacterial virulence factors including fimbriae, and porins [14,15]. Also, less purified OMP fractions have already been shown to be immunogenic in channel catfish [16][17][18][19]. This study is the first to use AL-93-75, a virulent isolate of E. ictaluri as the source of a highly purified OMP fraction.…”

Section: Introductionmentioning

confidence: 93%

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Immune response induced by N-lauroylsarcosine extracted outer-membrane proteins of an isolate of Edwardsiella ictaluri in channel catfish

Bader

Shoemaker

Klesius

2004

Fish & Shellfish Immunology

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

Immune response induced by N-lauroylsarcosine extracted outer-membrane proteins of an isolate of Edwardsiella ictaluri in channel catfish

Bader

Shoemaker

Klesius

2004

Fish & Shellfish Immunology

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“…OPS is attached to a core oligosaccharide (COS), which in turn is attached to the nonpolar component of LPS, lipid A. OPS composition and structure is highly species-and serotype-specific, and OPS is the immunodominant antigen of most Gramnegative bacteria, including Ed. ictaluri (Baldwin et al, 1997;Moore & Thune, 1999). Ed.…”

Section: Introductionmentioning

confidence: 99%

The Edwardsiella ictaluri O polysaccharide biosynthesis gene cluster and the role of O polysaccharide in resistance to normal catfish serum and catfish neutrophils

Lawrence

Banes

Azadi³

et al. 2003

Microbiology

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Edwardsiella ictaluri, the causative agent of enteric septicaemia of catfish (ESC), expresses long O polysaccharide (OPS) chains on its surface. The authors previously reported the construction of an isogenic Ed. ictaluri OPS mutant strain and demonstrated that this strain is avirulent in channel catfish. This paper reports the cloning of the Ed. ictaluri OPS biosynthesis gene cluster and identification of the mutated gene in the OPS-negative strain. The sequenced region contains eight complete ORFs and one incomplete ORF encoding LPS biosynthesis enzymes. The mutated gene (designated wbiT ) was similar to other bacterial galactose-4-epimerases. Glycosyl composition analysis indicated that wild-type Ed. ictaluri OPS contains higher amounts of galactose and N-acetylgalactosamine than the OPS mutant strain, which correlated well with predicted functions of the genes identified in the OPS biosynthesis cluster. The OPS mutant had a relatively small, but significant, decrease in its ability to survive in normal catfish serum compared to wild-type Ed. ictaluri, but it retained the ability to resist killing by catfish neutrophils.

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“…These vaccines have been shown to possess high immunogenicity and protect the catfish from ESC (Bader et al, 2004;Panangala et al, 2009b). The antigenicity of E. ictaluri has been studied (Baldwin et al, 1997;Dumpala et al, 2009) and has revealed many antigenic components, including flagella (Panangala et al, 2009a), extracellular capsular polysaccharides (Stanley et al, 1994), lipopolysaccharide (LPS) (Lawrence et al, 2001Williams et al, 2003), outer membrane proteins (OMPs) (Bader et al, 2004;Moore et al, 2002;Newton et al, 1990;Vinitnantharat et al, 1993), hemolysins (Williams and Lawrence, 2005), and chondroitinase (Cooper et al, 1996). However, little is known about the virulence factors of E. ictaluri responsible for antibody effectiveness (Panangala et al, 2009b).…”

Section: Vaccine Efficacy Of Glyceraldehyde-3-phosphate Dehydrogenasementioning

confidence: 99%

Vaccine efficacy of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Edwardsiella ictaluri against E. tarda in tilapia

Cao¹,

Tsai²,

Yang

et al. 2014

J. Gen. Appl. Microbiol.

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Glyceraldehyde-3-phosphate dehydrogenase (GAP-DH), derived from the outer-membrane protein (OMP) fraction, has been used as a potential candidate for vaccine development. The gene-encoding 37 kDa GAPDH outer membrane protein (OMP) from Edwardsiella ictaluri was amplified using polymerase chain reaction (PCR) and was cloned and expressed in Escherichia coli BL21 (DE3). Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting, and nucleotide and amino acid sequencing were used to analyze the expressed antigenic protein and gene encoding this protein. Comparative DNA and protein sequence analysis of GAPDH from E. ictaluri GAPDHs from several Gram-negative bacterial species within the Enterobacteriaceae family revealed that the GAPDHs within this group are highly conserved and share a sequence similarity of 75 100% with E. ictaluri GDPDH. Rabbit antiserum raised against the E. ictaluri recombinant GAPDH (rGAPDH) protein recognized purified GADPH, indicating that it has a strong immunogenicity. Tilapia fish were intraperitoneally immunized with formalin-killed E. ictaluri whole cells, and rGAPDH (30 µg fish 1 ) from E. ictaluri, both of which were emulsified in ISA 763A adjuvant. At 3 months after immunization, fish were challenged with the E. tarda strain to assess vaccine efficacy; the relative percent survival (RPS) values were found to exceed 71.4%. The specific mean antibody titer log 2 level of groups vaccinated with rGAPDH at 3 months was significantly higher than that of non-vaccinated fish (control group). Therefore, this recombinant protein can be considered a multi-purpose candidate vaccine against several pathogenic bacteria.Key words: channel catfish; Edwardsiella ictaluri; enteric septicemia of catfish; glyceraldehyde-3-phosphate dehydrogenase (GAPDH) IntroductionEnteric septicemia of catfish (ESC), caused by the bacterial pathogen Edwardsiella ictaluri, was first identified in channel catfish (Ictalurus punctatus) by Hawke (1979). E. ictaluri has become the most lethal infectious disease of channel catfish and other ictalurids in the USA (Plumb, 1999). More than 50% of American catfish fry/fingerling losses are due to ESC (Williams and Lawrence, 2010). E. ictaluri was also isolated from cultured fish species in Indonesia, Vietnam, Japan, Turkey (Crumlish et al., 2002;Keskün et al., 2004;Nagai et al., 2008; Yuas et al., 2003) and China (Ye et al., 2009). ESC is currently controlled using antibiotics; however, extensive use of antimicrobial com pounds reduces their effectiveness by increasing the number of antibiotic-resistant bacterial strains, resulting in the need for higher dosages for effective treatment as well as higher residual amounts of drugs in the harvested fish Thompson and Adams, 2004). Vaccines that protect fish against bacterial diseases are an important alternative to antibiotics and are a safer strategy for disease prevention. Most vaccines for controlling ESC have been tested using Full Paper Vaccine efficacy of glyceraldehyde-3-phosphate dehydrogenase (G...

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Antigens ofEdwardsiella ictalurirecognised by serum antibodies from naturally infected channel catfish

Cited by 18 publications

References 11 publications

Immune response induced by N-lauroylsarcosine extracted outer-membrane proteins of an isolate of Edwardsiella ictaluri in channel catfish

Immune response induced by N-lauroylsarcosine extracted outer-membrane proteins of an isolate of Edwardsiella ictaluri in channel catfish

The Edwardsiella ictaluri O polysaccharide biosynthesis gene cluster and the role of O polysaccharide in resistance to normal catfish serum and catfish neutrophils

Vaccine efficacy of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Edwardsiella ictaluri against E. tarda in tilapia

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