Antigens of Trypanosoma cruzi with clinical interest cloned and expressed in Escherichia coli

Silveira, José Franco da; Paranhos, Gláucia da Silva; Cotrim, Paulo C.; Mortara, Renato Arruda; Camargo, M. E.; Rassi, Anis; Wanderley, Jamiro S.; Corral, Ricardo S.; Freilij, Héctor; Grinstein, S; Degrave, Win

doi:10.1590/s0074-02761990000400022

Cited by 4 publications

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“…Cloned peptides carrying well defined antigenic determinants can be produced on a large scale and with an acceptable degree of purity. Recently, we described the characterization of a recombinant phage (H49) that encodes an immunodominant T. cruzi antigen consisting of tandem repeats of 68 amino acids , Da Silveira et al 1990). Evaluation of the H49 antigen as a reagent for serodiagnosis has been made by phage dot immunoassay with satisfactory results , Da Silveira et al 1990, Levin et al 1991.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, we described the characterization of a recombinant phage (H49) that encodes an immunodominant T. cruzi antigen consisting of tandem repeats of 68 amino acids , Da Silveira et al 1990). Evaluation of the H49 antigen as a reagent for serodiagnosis has been made by phage dot immunoassay with satisfactory results , Da Silveira et al 1990, Levin et al 1991. Antibodies against H49 antigen were detected in a large number of serum samples from chagasic patients, but not in sera from patients with other parasitic diseases.…”

Section: Introductionmentioning

confidence: 99%

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Detection of antibodies in sera from Chagas' disease patients using a Trypanosoma cruzi immunodominant recombinant antigen

Paranhos-Bacalla¹,

Santos²,

Cotrim³

et al. 1994

Parasite Immunology

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A Trypanosoma cruzi DNA fragment encoding an immunodominant repetitive antigen (H49) was subcloned into a protein purification and expressed system. Purified H49 peptide reacted specifically in an enzyme-linked immunosorbent assay (ELISA) with sera from T. cruzi-infected patients, but not with sera from patients with other parasitic diseases such as leishmaniasis and T. rangeli-infection. The H49 recombinant ELISA was able to detect specific antibodies in 84% of chronic chagasic serum samples tested. One of the major advantage of the recombinant ELISA for serodiagnosis of chronic Chagas' disease resides in its high specificity (100%). Our data suggest that recombinant peptides could provide a practical basis for specific diagnosis tests for Chagas' disease.

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Section: Introductionmentioning

confidence: 99%