Antigens of Murine Melanoma and Their Cross-Species Reactivity

Gersten, Douglas M.; Hearing, Vincent J.

doi:10.1159/000163697

Cited by 8 publications

(2 citation statements)

References 32 publications

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“…Within the field of melanoma, a great deal of data has been accumulated on cross-species antigens (review, Gersten and Hearing, 1992a), especially B700. Thus, om initial experiments, which suggested that B700 might be eliminated intact from tumor into the urine, were of particular interest in the context of biomarkers (Farzaneh et al, 1991b).…”

Section: Discussionmentioning

confidence: 99%

“…Lastly, an antigenically and structurally similar protein to B700, termed H700, has been detected in the serum and urine of melanoma patients (Tomecki et al, 1980). There is as yet no animal model for studying urinary excretion of melanoma antigens; the subject of cross species melanoma antigens has been recently reviewed (Gersten and Hearing, 1992a). The complete sequence of I3700 is not yet known, but amino acid analysis (Marchalonis et al, 1984), N-terminal sequence (Marchalonis et al, 1984), and serological cross-reactivity studies (Gersten et al, 1991) place B700 in the serum albumin fam-ily.…”

Section: Introductionmentioning

confidence: 99%

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Proteinuria of B700, a 67 kD Albumin‐like Melanoma‐Specific Antigen

Walden

Farzaneh

Hearing

et al. 1992

Pigment Cell Research

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B700 is a murine melanoma antigen that is closely related to, but distinct from, serum albumin. The present study examined the metabolic fate and anatomic distribution of radioiodinated B700 and mouse serum albumin (MSA) administered s.c. to mice. In blood, both proteins were associated with the plasma fraction where the halflife of B700, a glycoprotein, was 0.5 days, compared to 2.7 days for MSA. Of particular interest was the observation that B700, a 67 kD anionic protein, was excreted primarily in urine. The selective B700-proteinuria did not alter urinary volumes or produce hematuria or edema. SDS-polyacrylamide gel electrophoresis and western blot analysis using the H-2-3-3 B700-specific monoclonal antibody revealed that B700 proteinuria occurred in B-16 murine melanoma bearing animals but not in control mice. These studies demonstrate that the tumor-bearing host readily distinguishes between very similar normal protein (MSA) and tumor-associated antigen (B700) molecules and processes them differently.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Proteinuria of B700, a 67 kD Albumin‐like Melanoma‐Specific Antigen

Walden

Farzaneh

Hearing

et al. 1992

Pigment Cell Research

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Expression of autocrine motility factor receptor in serum‐ and protein‐independent fibrosarcoma cells: Implications for autonomy in tumor‐cell motility and metastasis

Watanabe

Shinozaki

Raz

et al. 1993

Intl Journal of Cancer

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The motile response of serum-dependent (Gc-4 SD) and protein-independent (Gc-4 PF) murine fibrosarcoma cells to monoclonal antibody (MAb) that binds to gp78 a cell-surface receptor (M(r) 78,000) for an autocrine motility factor (AMF) was analyzed. The Gc-4 PF cells responded to the anti-gp78 by increased motility in vitro (3-fold) and increased lung colonization in vivo (8- to 20-fold), while the serum-dependent counterpart failed to respond to motile stimulation both in vitro and in vivo. Immuno-analysis of cell-surface expression and cell extracts revealed a smaller amount of gp78 in Gc-4 SD cells than in Gc-4 PF cells. Both cell lines secrete an equal amount of AMF to the culture media. Our results suggest that protein-free culture of Gc-4 PF cells is associated with high response to AMF and with high expression of its receptor, and that autonomous motile regulation may play a role in tumor dissemination.

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Generation of cytotoxic antibodies to the B16 murine melanoma using a formalinized vaccine

Shrayer

Kouttab

Maizel

et al. 1993

Intl Journal of Cancer

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The goal of our experiments was to determine the extent to which the humoral response to a melanoma vaccine elicits the production of cytotoxic antibodies in tumor-challenged mice. Mice were immunized with a vaccine produced from formalinized extracellular antigens (FECA) derived from B16 F10 melanomas. The production of antibodies that recognized the vaccine preparation was determined by ELISA, as was their cross-reactivity with the B700 melanoma antigen. The antibodies were shown to be anti-proliferative by inhibition of tritiated thymidine incorporation into the DNA of cultured target cells and cytotoxic by assays for complement-mediated and antibody-dependent cellular cytotoxicity. Flow cytometric analyses indicated that approximately 60% of the target cells specifically bound antibody from the immune sera. These results confirm that B700 is a significant antigenic component of the FECA vaccine, and provide encouragement for this approach to developing useful melanoma vaccines.

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Antigens of Murine Melanoma and Their Cross-Species Reactivity

Cited by 8 publications

References 32 publications

Proteinuria of B700, a 67 kD Albumin‐like Melanoma‐Specific Antigen

Proteinuria of B700, a 67 kD Albumin‐like Melanoma‐Specific Antigen

Expression of autocrine motility factor receptor in serum‐ and protein‐independent fibrosarcoma cells: Implications for autonomy in tumor‐cell motility and metastasis

Generation of cytotoxic antibodies to the B16 murine melanoma using a formalinized vaccine

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