Antigens of Aeromonas salmonicida subsp. salmonicida specifically induced in vivo in Oncorhynchus mykiss

Menanteau–Ledouble, Simon; El-Matbouli, Mansour

doi:10.1111/jfd.12430

Cited by 17 publications

(11 citation statements)

References 24 publications

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“…Aeromonas are well‐known fish pathogens (Beaz‐Hidalgo & Figueras, ). The species with the highest clinical significance is Aeromonas salmonicida, the causative agent of furunculosis, a major disease with high mortality rates that causes great economic losses in aquaculture, particularly affecting salmonids (Beaz‐Hidalgo, Alperi, Bujan, Romalde & Figueras, ; Khor, Puah, Tan, Puthucheary & Chua, ; Menanteau‐Ledouble & El‐Matbouli, ). Other species, such as A hydrophila , Aeromonas bestiarum , A. veronii and Aeromonas sobria have also been associated with diseases in fish (Beaz‐Hidalgo & Figueras, ; Kozinska, ; Orozova, Barker, Austin & Austin, ; Rasmussen‐Ivey et al., ; Yi et al., ).…”

Section: Introductionmentioning

confidence: 99%

Limited performance of MALDI‐TOF for identification of fish Aeromonas isolates at species level

Cerda

Fernández-Bravo

Domı́nguez³

et al. 2018

Journal of Fish Diseases

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The aim of this study was to evaluate the usefulness of the MALDI-TOF MS to identify 151 isolates of Aeromonas obtained mostly from diseased fish. MALDI-TOF MS correctly identified all isolates to the genus level but important differences in the percentage of isolates correctly identified depending on the species were observed. Considering exclusively the first identification option, Aeromonas bestiarum, Aeromonas hydrophila, Aeromonas salmonicida, Aeromonas veronii and Aeromonas sobria were the best identified with results >95%. However, considering the first and second identification options, the only species that showed values >90% was A. hydrophila. Overall, when the database was supplemented with 14 new spectra, the number of accurate identifications increased (41% vs. 55%) and the number of inconclusive identifications decreased (45% vs. 29%), but great differences in the success of species-level identifications were found. Species-distinctive mass peaks were identified only for A. hydrophila and A. bestiarum (5003 and 7360 m/z in 95.5% and 94.1% of their isolates, respectively). This work demonstrates the utility of MALDI-TOF MS for Aeromonas identification to the genus level, but there is no consistency for the accurate identification of some of the most prevalent species implicated in fish disease.

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Section: Introductionmentioning

confidence: 99%

Limited performance of MALDI‐TOF for identification of fish Aeromonas isolates at species level

Cerda

Fernández-Bravo

Domı́nguez³

et al. 2018

Journal of Fish Diseases

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“…The T3SS of A. salmonicida is likely its most important virulence factor [ 134 ] but was only relatively recently reported, its discovery probably hindered by the fact that T3SS are lost when the bacterium is cultivated above 20 °C [ 135 – 137 ], due to the T3SS gene cluster being locating in a thermosensitive region [ 138 ]. Among the most important effector proteins secreted by the T3SS of A. salmonicida are AopH, Ati2, AexT, AopP, AopO, AopN and ExsE [ 134 ], that have been associated with cytoskeletal collapse and immune system response impairment [ 139 ], with aopO being upregulated during the infectious process [ 140 ]. Moreover, AcrV and AopB are homologues in LcrV and YopB in Yersinia which stimulate IL-10 production in the cell, consequently supressing the immune response [ 141 ].…”

Section: T3ss In Aquatic Pathogensmentioning

confidence: 99%

Delivering the pain: an overview of the type III secretion system with special consideration for aquatic pathogens

Rahmatelahi

El‐Matbouli

Menanteau–Ledouble

2021

Vet Res

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Gram-negative bacteria are known to subvert eukaryotic cell physiological mechanisms using a wide array of virulence factors, among which the type three-secretion system (T3SS) is often one of the most important. The T3SS constitutes a needle-like apparatus that the bacterium uses to inject a diverse set of effector proteins directly into the cytoplasm of the host cells where they can hamper the host cellular machinery for a variety of purposes. While the structure of the T3SS is somewhat conserved and well described, effector proteins are much more diverse and specific for each pathogen. The T3SS can remodel the cytoskeleton integrity to promote intracellular invasion, as well as silence specific eukaryotic cell signals, notably to hinder or elude the immune response and cause apoptosis. This is also the case in aquatic bacterial pathogens where the T3SS can often play a central role in the establishment of disease, although it remains understudied in several species of important fish pathogens, notably in Yersinia ruckeri. In the present review, we summarise what is known of the T3SS, with a special focus on aquatic pathogens and suggest some possible avenues for research including the potential to target the T3SS for the development of new anti-virulence drugs.

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“…(Sha et al, 2007) Putative serine/threonine kinase. (Dacanay et al, 2006;Menanteau--Ledouble & El-Matbouli, 2016;Bartkova et al, 2017) Disturbs the normal distribution of actin in the host cell. (Groves et al, 2010) ati2 T3SS Suspected of having inositol polyphosphate phosphatase activity.…”

Section: T3ss Secretion System and Effector Proteinsmentioning

confidence: 99%

Role of Bacterial Secretion Systems and Effector Proteins – Insight into the Pathogenicity Mechanisms in Aeromonas

2020

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Gram-negative bacteria have developed several nanomachine channels known as type II, III, IV and VI secretion systems that enable export of effector proteins/toxins from the cytosol across the outer membrane to target host cells. Protein secretion systems are critical to bacterial virulence and interactions with other organisms. Aeromonas utilize various secretion machines e.g. two-step T2SS, a Sec-dependent system as well as one-step, Sec-independent T3SS and T6SS systems to transport effector proteins/toxins and virulence factors. Type III secretion system (T3SS) is considered the dominant virulence system in Aeromonas. The activity of bacterial T3SS effector proteins most often leads to disorders in signalling pathways and reorganization of the cell cytoskeleton. There are also scientific reports on the pathogenicity mechanism associated with host cell apopotosis/pyroptosis resulting from secretion of a cytotoxic enterotoxin, i.e. the Act protein, by the T2SS secretion system and an effector protein Hcp by the T6SS system. Type IV secretion system (T4SS) is the system which translocate protein substrates, protein-DNA complexes and DNA into eukaryotic or bacterial target cells. In this paper, the contribution of virulence determinants involved in the pathogenicity potential of Aeromonas is discussed. Considering that the variable expression of virulence factors has a decisive impact on the differences observed in the virulence of particular species of microorganisms, it is important to assess the correlation between bacterial pathogenicity and their virulence-associated genes.

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Antigens of Aeromonas salmonicida subsp. salmonicida specifically induced in vivo in Oncorhynchus mykiss

Cited by 17 publications

References 24 publications

Limited performance of MALDI‐TOF for identification of fish Aeromonas isolates at species level

Limited performance of MALDI‐TOF for identification of fish Aeromonas isolates at species level

Delivering the pain: an overview of the type III secretion system with special consideration for aquatic pathogens

Role of Bacterial Secretion Systems and Effector Proteins – Insight into the Pathogenicity Mechanisms in Aeromonas

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