Antigens of Human T-Lymphotropic Virus Type III/Lymphadenopathy-Associated Virus

Essex, Max; Allan, Jonathan S.; Kanki, Phyllis J.; McLane, Mary Fran; Malone, G; Kitchen, Lynn W.; Lee, T.H.

doi:10.1016/s0022-5347(17)44960-3

Cited by 2 publications

(2 citation statements)

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“…However, when measured by immunoprecipitation of radioiodinated gp120, considerable activity was also noted in the effluent fraction (not shown). This confirms earlier conclusions that radioimmunoprecipitation analyses are the most sensitive and accurate measure of antibodies to the gp120 (19) (see Discussion for further elaboration of this point).…”

Section: Resultssupporting

confidence: 90%

Restricted neutralization of divergent human T-lymphotropic virus type III isolates by antibodies to the major envelope glycoprotein.

Matthews¹,

Langlois²,

Robey³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

186

104

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By analogy to other retroviruses, the major envelope glycoprotein, gpl20, of human T-lymphotropic virus type III (HTLV-Ill) is a probable target for neutralizing antibody. This antigen has been purified from H9 cells chronically infected with the HTLV-IllB prototype strain. Several goats immunized with the gpl20 produced antibodies that neutralized infection of H9 cells by the homologous virus isolate. These same sera failed to neutralize the divergent HTLV-IllRF isolate. Individuals infected with HTLV-11I commonly develop antibodies to gpl20 that could be isolated by using the gpl20 antigen coupled to an immunoadsorbent resin. The antibody fraction that bound tightly to such a resin was found to neutralize the IIIB but not the RF isolate in a similar fashion as the goat anti-gpl20 sera. However, the nonbinding fraction (effluent) from the resin also contained neutralizing activity that was able to block infection by both virus isolates with similar efficacy. Human antibodies to the other virus envelope gene product, the transmembrane gp4l, were also affinity purified by utilizing the recombinant peptide 121, but these failed to influence infection by either virus isolate.

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Section: Resultssupporting

confidence: 90%

Restricted neutralization of divergent human T-lymphotropic virus type III isolates by antibodies to the major envelope glycoprotein.

Matthews¹,

Langlois²,

Robey³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

186

104

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“…One of these tests, the Western blot (WB), is considered to be both nearly 100% sensitive and specific, and is used as a gold standard for the other tests. Despite knowledge that cellular constituents and/or fragments of the same buoyant density as retroviral particles may contaminate the supernatants of cell cultures (Papadopulos-Eleopulos, Turner & Papadimitriou, 1993a), material for the WB is obtained by density gradient centrifugarion of the supernatant from 'HIV infected' cell cultures or even cell lysates, the latter being the case in the first 'HIV isolation' ( Barr6-Sanoussi et al, 1983), and subsequently in other laboratories (Essex et al, 1985;Albert etal., 1988;Levinson & Denys, 1988). Material which bands at 1.16 gm/ml is considered to represent pure HIV and consequently the proteins found at this density are considered to be HIV antigens.…”

Section: Hiv and Aidsmentioning

confidence: 99%

A critical analysis of the HIV-T4-cell-AIDS hypothesis

Papadopulos-Eleopulos

Turner

Papadimitriou

et al. 1995

Genetica

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The data generally accepted as proving the HIV theory of AIDS, HIV cytopathy, destruction of T4 lymphocytes, and the relationship between T4 cells, HIV and the acquired immune deficiency clinical syndrome are critically evaluated. It is concluded these data do not prove that HIV preferentially destroys T4 cells or has any cytopathic effects, nor do they demonstrate that T4 cells are preferentially destroyed in AIDS patients, or that T4 cell destruction and HIV are either necessary or sufficient prerequisites for the development of the clinical syndrome.

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Antigens of Human T-Lymphotropic Virus Type III/Lymphadenopathy-Associated Virus

Cited by 2 publications

References 0 publications

Restricted neutralization of divergent human T-lymphotropic virus type III isolates by antibodies to the major envelope glycoprotein.

Restricted neutralization of divergent human T-lymphotropic virus type III isolates by antibodies to the major envelope glycoprotein.

A critical analysis of the HIV-T4-cell-AIDS hypothesis

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