Antigens of gastric and intestinal mucous cells in human colonic tumours

Abstract

Summary.-Using immunofluorescence methods, 3 antisera respectively stain 3 groups of mucous cells of the human gastrointestinal tract, showing specific antigens for each group of cells.The antigens of the first group, the MI antigens, were principally associated with columnar cells of the gastric epithelium, the M2 antigens with mucous cells of gastric and Brunner's glands, and the M3 antigen with the goblet cells of the intestinal mucosa.The gastric M antigens normally detectable in stomach and duodenum (but not in colon) were expressed in certain colonic tumours (benign or malignant) and in adjacent mucosa. They are always present with the intestinal M3 antigen. In 100 colonic adenocarcinomas, the intestinal M3 antigen was found in 53 cases, gastric Ml antigens in 29 cases, and gastric M2 antigens in 10 cases, always with the two other M antigens. A good correlation could be established between the association of M antigens and the histological type of tumour.

Human colonic goblet cells. Demonstration of distinct subpopulations defined by mucin-specific monoclonal antibodies.

“…Despite the differences between patterns of mucin heterogeneity observed with anti-HCM MAbs and other methods within the colonic mucosa, the increasing cross-reactivity ofanti-HCM MAbs at progressively more distal sites of the gastrointestinal tract is comparable to the results of earlier studies (29,34). The present studies confirm the impression that some structural features are common to all gastrointestinal tract mucin, while other components of mucin may represent organ specific determinants.…”

Development of anti-human colonic mucin monoclonal antibodies. Characterization of multiple colonic mucin species.

“…Indeed, several earlier studies have utilized probes directed to circumscribed nonreducing terminal structures to explore colonic mucin heterogeneity. These probes have included lectins that recognize terminal monosaccharides or disaccharides (18,22,33,34), histochemical stains that bind peripheral charged residues (1 1, 30, 35, 36) or conventional antisera directed at limited peripheral carbohydrate determinants (17,37,38). The present results suggest that relatively nonspecific tools may both underestimate heterogeneity and overestimate structural homologies.…”

Phenotypic Expression of Colorectal Adenocarcinomas with Reference to Tumor Development and Biological Behavior

“…[15][16][17][18] HGM is a specific marker for core protein of gastric foveolar cells. 19,20) The correlations between positivity to these antibodies and clinicopathologic features suggested that cell differentiation is inversely associated with tumor growth and aggressiveness.…”

“…We selected three immunohistochemical markers for cell differentiation: MUC2 for intestinal goblet cells, [12][13][14] CD10 for small intestinal brush border [15][16][17][18] and human gastric mucin (HGM) for gastric differentiation. 19,20) A representative section from each tumor was processed for immunohistochemical staining with monoclonal antibodies against MUC2 (Novocastra, Newcastle-upon-Tyne, UK, diluted 1:200), CD10 (Novocastra, diluted 1:200) and HGM (45M1, Novocastra, diluted 1:50). Four-micron-thick sections were cut, deparaffinized in xylene and dehydrated in an ethanol series.…”