Antigens and glycoproteins of larvae, nymphs and adults of the tick Ixodes ricinus

Uhlír, J; Grubhoffer, Libor; Borský, I.; Dusbábek, F.

doi:10.1111/j.1365-2915.1994.tb00154.x

Cited by 15 publications

(16 citation statements)

References 27 publications

(15 reference statements)

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“…Differences in the antigenic profiles of whole homogenates (larvae and nymphs) or SGE with serum from rabbits or hamsters infested by different developmental stages of I. ricinus indicate that the antigens exposed to a host are dependent upon the developmental stage feeding. These results are consistent with previous studies using I. ricinus ticks fed on rabbits (26,27). The majority of antigens present in the whole homogenate samples were also present in SGE immunoprobed with the relevant serum, suggesting that the salivary glands are the major source of antigens exposed during tick infestation.…”

Section: Discussionsupporting

confidence: 92%

Antigenic profile of Ixodes ricinus: effect of developmental stage, feeding time and the response of different host species

Lawrie¹,

Nuttall²

2001

Parasite Immunology

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Antigens recognized by host species in response to ectoparasite infestation have been widely reported. Although differences in the immune responses of different host species have been described, only a very few of these studies compare the range of antigens recognized by different host species in response to infestation. We used Western blot analysis to investigate antigenic responses of different host species that were repeatedly infested with Ixodes ricinus ticks. Antigenic profiles of larval and nymphal whole tick homogenates were compared with the respective salivary gland extract (SGE) samples using sera from rabbits repeatedly infested with either adults, nymphs or larvae. SGE samples were also analysed using sera from hamsters infested with adults, nymphs or larvae. Sera from BALB/C mice, Apodemus flavicollis (yellow-necked mouse) or Clethrionomys glareolus (bank vole) repeatedly infested with larvae were used to compare the antigenic profiles of SGE and larval homogenate samples. We also investigated different sources of tick antigens, using rabbit sera, by comparing midgut extracts from female adult ticks and SGE from unfed ticks and from ticks throughout the 6-day feeding period with whole tick homogenates of female and male adults, nymphs and larvae. The pattern of antigenic tick-molecules recognized by infested host species varies with the period of feeding, developmental stage and the particular host species parasitized.

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Section: Discussionsupporting

confidence: 92%

Antigenic profile of Ixodes ricinus: effect of developmental stage, feeding time and the response of different host species

Lawrie¹,

Nuttall²

2001

Parasite Immunology

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“…The innate humoral immune response that resistant hosts present upon a primary exposure to larvae may contain the infestations and increase the immunogenicity of salivary components. In humans, natural antibodies appear to be important in the immune responses against pathogens, since they have a high affinity for carbohydrate groups present in the membrane of many pathogens [81] and tick salivary proteins are glycosylated [82]. Another finding in this study is that, at the second successive infestation, the levels of total IgE increased in sera from both susceptible and resistant hosts and then decreased significantly at the third infestation.…”

Section: Discussionmentioning

confidence: 60%

Immune recognition of salivary proteins from the cattle tick Rhipicephalus microplus differs according to the genotype of the bovine host

et al. 2017

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Background: Males of the cattle tick Rhipicephalus microplus produce salivary immunoglobulin-binding proteins and allotypic variations in IgG are associated with tick loads in bovines. These findings indicate that antibody responses may be essential to control tick infestations. Infestation loads with cattle ticks are heritable: some breeds carry high loads of reproductively successful ticks, in others, few ticks feed and they reproduce inefficiently. Different patterns of humoral immunity against tick salivary proteins may explain these phenotypes.

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“…1993 ). On the other hand, oligosaccharides with both N‐ and O‐type glycans were found in the salivary glands of ticks Argas polonicus ( Grubhoffer & Dusbábek, 1991) and Ixodes ricinus ( Uhlíř et al . 1994 ).…”

Section: Discussionmentioning

confidence: 99%

Salivary proteins and glycoproteins in phlebotomine sandflies of various species, sex and age

Volf¹,

Tesařová

Nohýnková

2000

Medical Vet Entomology

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Salivary gland proteins were studied in sandflies (Diptera: Psychodidae: Phlebotominae) by electrophoretic techniques. In Phlebotomus duboscqi Neveu-Lemaire the protein concentration was about 30 times higher in females than in males. SDS PAGE revealed eight major bands of 29-62 kDa in salivary gland extracts (SGE) from females, whereas only one band of 57kDa was detected in males. The number of protein components in SGE gradually increased with the age of females. In P. papatasi (Scopoli) the typical electrophoretic pattern was reached in 3-5 days after imago emergence, depending on the temperature at which females were maintained. All major protein components of the female SGE were present in the content of glands. Female SGE were compared in seven colonies of five Phlebotomus and Lutzomyia species; electrophoretic profiles distinguished between species and even between colonies of different geographical origin. In general, the highest variability of major protein components was observed in the 38-48kDa region. Four colonies of the subgenus Phlebotomus (P. duboscqi and P. papatasi) possessed common mobility polypeptides, the highest similarity was found between two colonies of P. papatasi. Other species tested significantly differed, specific prominent bands of 33, 35 and 38kDa were found in P. halepensis Theodor, P. perniciosus Newstead and Lutzomyia longipalpis (Lutz & Neiva), respectively. Glycoproteins in SGE of Lu. longipalpis and P. duboscqi females were identified and analysed using blotting with five lectin conjugates. Specific reaction of lectins ConA and WGA revealed the complex type of N-glycans in the 48 and 53-54kDa glycoproteins present in both species. Similar glycosylation was detected in species-specific bands of the 57-60 and 65-67 kDa in P. duboscqi and Lu. longipalpis, respectively. The high mannose type of glycosylation was found in the 20 and 39 kDa polypeptides of Lu. longipalpis and the 40-42 kDa polypeptides of P. duboscqi. Innate lectin activity specific for aminosugars was detected in SGE of P. duboscqi females using haemagglutination tests with rabbit erythrocytes.

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Antigens and glycoproteins of larvae, nymphs and adults of the tick Ixodes ricinus

Cited by 15 publications

References 27 publications

Antigenic profile of Ixodes ricinus: effect of developmental stage, feeding time and the response of different host species

Antigenic profile of Ixodes ricinus: effect of developmental stage, feeding time and the response of different host species

Immune recognition of salivary proteins from the cattle tick Rhipicephalus microplus differs according to the genotype of the bovine host

Salivary proteins and glycoproteins in phlebotomine sandflies of various species, sex and age

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