Antigenotoxicity of Agaricus blazei mushroom organic and aqueous extracts in chromosomal aberration and cytokinesis block micronucleus assays in CHO-k1 and HTC cells

Bellini, Marilanda Ferreira; Angeli, Jpf; Matuo, Renata; Terezan, Ana Paula; Ribeiro, Lúcia Regina; Mantovani, Mário Sérgio

doi:10.1016/j.tiv.2005.08.009

Cited by 49 publications

(37 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HTC cell lineage is a drug-metabolizing cell line that has been used for the direct and indirect identification of antigenotoxic agents (Bellini et al 2006;Oliveira et al 2006;Marcarini et al 2011). We investigated the protective effects of genistein and daidzein on DNA damage induced by doxorubicin (a direct-acting mutagen) and 2-aminoanthracene (an indirect-acting mutagen) on HTC cells by using the micronucleus test.…”

Section: Discussionmentioning

confidence: 99%

Chemoprotective activity of the isoflavones, genistein and daidzein on mutagenicity induced by direct and indirect mutagens in cultured HTC cells

et al. 2012

View full text Add to dashboard Cite

Isoflavones are phenolic compounds widely distributed in plants and found in a high percentage in soybeans. They have important biological properties and are regarded as potential chemopreventive agents. The aim of this study was to verify the preventive effect of two soy isoflavones (genistein and daidzein) by a micronucleus assay, analysis of GST activity, and real-time RT-PCR analysis of GSTa2 gene expression. Mutagens of direct (doxorubicin) and indirect (2-aminoanthracene) DNA damage were used. Hepatoma cells (HTC) were treated with genistein or daidzein for 26 h at noncytotoxic concentrations; 10 lM when alone, and 0.1, 1.0 and 10 lM when combined with genotoxic agents. The micronucleus test demonstrated that both isoflavones alone had no genotoxic effect. Genistein showed antimutagenic effects at 10 lM with both direct and indirect DNA damage agents. On phase II enzyme regulation, the current study indicated an increase in total cytoplasmic GST activity in response to genistein and daidzein at 10 lM supplementation. However, the mRNA levels of GSTa2 isozymes were not differentially modulated by genistein or daidzein. The results point to an in vitro antimutagenic activity of genistein against direct and indirect DNA damage-induced mutagenicity.

show abstract

Section: Discussionmentioning

confidence: 99%

Chemoprotective activity of the isoflavones, genistein and daidzein on mutagenicity induced by direct and indirect mutagens in cultured HTC cells

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Natural antioxidant compounds in the human diet may prevent the genetic effects of mutagens and carcinogens and many studies have shown that an increased intake of fruits or vegetables rich in antioxidants decreases the level of oxidative DNA damage (Duthie et al 1996;Collins et al 2001;Dusinská et al 2003). Extracts of the plant Toxicodendron quercifolium (poison-ivy, eastern poison-oak) and the mushroom Agaricus blazei (the Brazilian sun-mushroom) have shown antigenotoxic activity when examined using the in vivo mouse bone marrow micronucleus assay and the in vitro Chinese hamster ovary cell (CHO-K1) chromosome aberration assay (Mersch-Sundermann et al 2004;Guterres et al 2005;Bellini et al 2006). Edenharder et al (2002) used various test-systems for the evaluation of genotoxicity to demonstrate the protective effects of plant beverages, fruits, vegetables and spices against genotoxicity induced by 2-acetylaminofluorene or 2-amino-1-methyl-6-phenylimidazo [4,5-b]pyridine and Edenharder et al (2003) reported that homogenates of spinach, artichoke, peaches and black grapes reduced micronuclei (MN) induction in mouse bone marrow treated with benzo[a]pyrene.…”

Section: Introductionmentioning

confidence: 99%

Anticlastogenic potential and antioxidant effects of an aqueous extract of pulp from the pequi tree (Caryocar brasiliense Camb)

et al. 2007

View full text Add to dashboard Cite

The effectiveness of an aqueous extract of Caryocar brasiliense (Caryocaraceae) Camb pulp, popularly known in Brazil as pequi, against clastogenicity induced by cyclophosphamide and bleomycin was evaluated using an in vivo mouse bone marrow cell micronuclei test, an in vitro Chinese hamster ovary cell (CHO-K1) chromosome aberration test and an in vitro antioxidant assay based on the oxidative damage to 2-deoxy-D-ribose (2-DR) induced by hydroxyl radicals ( • OH) generated by the reaction between ascorbic acid and (Fe III)-EDTA. In mouse bone marrow cells the extract showed a protective effect against micronuclei induced by cyclophosphamide and bleomycin but did not interfere with polychromatic bone marrow erythrocyte proliferation, except when the mice had been treated with the highest dose of cyclophosphamide. When CHO-K1 cells were pretreated by adding 0.01, 0.05 or 0.1 mL of extract per mL of cell culture medium 24 or 48 h before bleomycin or cyclophosphamide there was a protective effect against chromosome breaks and a significant decrease in the mitotic index (a measure of cytotoxicity) of the CHO-K1 cells. The extract also had a protective effect against oxidative hydroxyl radical damage to 2-DR. This study suggests that C. brasiliense pulp aqueous extract has anticlastogenic potential, possibly due to its antioxidative properties.

show abstract

“…The antiproliferative effect of Agaricus mycelium has been described in other investigations, demonstrating protective effects against genetic damage (Barbisan et al, 2003;Bellini et al, 2006;Delmanto et al, 2001;Luiz, Jordao, Eira, Ribeiro, & Mantovani, 2003;Machado, Filho, Terezan, Ribeiro, & Mantovani, 2005;Martins, Jordao, Ribeiro, Ferreira, & Mantovani, 2002) and inhibitory effects on tumour cell growth, cell migration or tumour-induced neovascularisation by mushroom (Kimura, Kido, Takaku, Sumiyoshi, & Baba, 2004;Kobayashi et al, 2005;Takaku, Kimura, & Okuda, 2001). Regarding this, Kawamura and Kasai (2007) reported that hemicellulase-treated A. blazei showed a strong antiproliferative effect and induced p38 mitogen-activated protein kinase-mediated cell growth delay and apoptosis in some cell lines.…”

Section: Discussionmentioning

confidence: 55%

Agaricus brasiliensismycelium and its polysaccharide modulate the parameters of innate and adaptive immunity

Navegantes

Albuquerque

Dalla-Santa

et al. 2013

Food and Agricultural Immunology

View full text Add to dashboard Cite

The biological properties of Agaricus brasiliensis mainly include immunostimulating and antitumour activities. This study evaluated the immunomodulating effect of A. brasiliensis mycelium (LPB) and its exopolysaccharides (EPS) on immunological parameters, such as phagocytosis of Candida albicans and nitric oxide (NO) production by infected macrophages, splenocytes proliferation in response to C. albicans. It also assessed the effects of the intake of LPB on the number of lymphocytes in the lymph node and spleen of treated animals and the TNF-a production. It was showed that the LPB and EPS had opposite effects; LPB had antiproliferative action and also reduced the NO levels produced by macrophages. However, the EPS showed an immune-stimulating effect potentiating the splenocytes proliferation and NO production from macrophages after stimulation with C. albicans. In addition, it also protected the macrophages from death induced by yeast. These data confirmed the immunomodulatory properties of LPB and its polysaccharides.

show abstract

Antigenotoxicity of Agaricus blazei mushroom organic and aqueous extracts in chromosomal aberration and cytokinesis block micronucleus assays in CHO-k1 and HTC cells

Cited by 49 publications

References 16 publications

Chemoprotective activity of the isoflavones, genistein and daidzein on mutagenicity induced by direct and indirect mutagens in cultured HTC cells

Chemoprotective activity of the isoflavones, genistein and daidzein on mutagenicity induced by direct and indirect mutagens in cultured HTC cells

Anticlastogenic potential and antioxidant effects of an aqueous extract of pulp from the pequi tree (Caryocar brasiliense Camb)

Agaricus brasiliensismycelium and its polysaccharide modulate the parameters of innate and adaptive immunity

Contact Info

Product

Resources

About