Antigenotoxic, anti-photogenotoxic and antioxidant activities of natural naphthoquinone shikonin and acetylshikonin and Arnebia euchroma callus extracts evaluated by the umu-test and EPR method

Skrzypczak, Agata; Przystupa, Natalia; Zgadzaj, Anna; Parzonko, Andrzej; Sykłowska-Baranek, Katarzyna; Paradowska, Katarzyna; Nałęcz‐Jawecki, Grzegorz

doi:10.1016/j.tiv.2015.09.029

Cited by 32 publications

(20 citation statements)

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“…Although EXTs revealed an antioxidative potential, the increase in the 4NQO-induced genotoxicity was observed. Previously, it was reported that antioxidants did not exhibit a protective effect against 4NQO [36] or an increase in the genotoxicity [37]. This could be explained by the fact that the oxidative stress is not the only possible mechanism of action of this mutagen.…”

Section: Discussionmentioning

confidence: 96%

Antigenotoxic, Anti-photogenotoxic, and Antioxidant Properties of Polyscias filicifolia Shoots Cultivated In Vitro

et al. 2020

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Traditional medicinal plants are an important source of active compounds with potential antimutagenic activity. Polyscias filicifolia Bailey (Araliaceae) is a South Asian traditional herb used as an adaptogenic and cardiac drug. Extracts of P. filicifolia contain a wide range of biologically active compounds like phenolic acids and triterpenoid saponins. In the present study. antigenotoxic potential of three naturally occurring phenolic acids and extracts of P. filicifolia growing in vitro with the addition of elicitors was evaluated against direct (4-nitroquinoline-N-oxide (4NQO) and mitomycin C (MMC)) and indirect mutagens (2-aminoanthracene (2AA)). The evaluation was made using a bacterial umu-test. Moreover, the ability to prevent photogenotoxicity induced by chlorpromazine (CPZ) under UVA irradiation was measured. The phytochemical profiling of examined extracts revealed the presence of numerous compounds with the prevelance of chlorogenic, caffeic, and ferulic acid derivatives; however, saponin fractions were also determined. The antioxidant potential of extracts strictly correlated with their composition. The tested extracts exhibited high antigenotoxic activity if the assay was performed with 2AA and metabolic activation. Moreover, the extracts slightly decreased the MMC-induced genotoxicity. However, an increase of the genotoxic effect was observed in the assay performed with 4NQO. In addition, photo-antigenotoxic activity was observed. In our study, phenolic acids exhibited lower activity than the extracts.

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Section: Discussionmentioning

confidence: 96%

Antigenotoxic, Anti-photogenotoxic, and Antioxidant Properties of Polyscias filicifolia Shoots Cultivated In Vitro

et al. 2020

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“…Acetylshikonin, a major bioactive component of leaf extracts of L . erythrorhizon root (Gwon et al, 2012; Pietrosiuk et al, 2006; Zhao, Wu, Wu, Zhao, & Chen, 2016), exhibits various pharmacological activities including induction of autophagy, anti‐oxidative, anti‐inflammatory, anti‐proliferative, anti‐fertility, and anticancer effects (He, Li, Su, Huang, & Zhu, 2016; Pietrosiuk et al, 2006; Skrzypczak et al, 2015; Wu et al, 2011; Zeng, Zhu, & Su, 2018). Several studies have demonstrated that the compound inhibits growth of a variety of cancers, including colorectal, breast, liver, medullary thyroid carcinoma, pancreas, melanoma, and gastric cancers (Cho & Choi, 2015; Hasenoehrl et al, 2017; Kim, Lee, Park, & Choi, 2016; Kretschmer et al, 2012; Park et al, 2017; Vukic et al, 2017; Zeng, Liu, & Zhou, 2009).…”

Section: Discussionmentioning

confidence: 99%

Acetylshikonin suppressed growth of colorectal tumour tissue and cells by inhibiting the intracellular kinase, T‐lymphokine‐activated killer cell‐originated protein kinase.

Zhao

Choi

Wei

et al. 2020

British J Pharmacology

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Background and Purpose: Overexpression or aberrant activation of the T-lymphokine-activated killer cell-originated protein kinase (TOPK) promotes gene expression and growth of solid tumours, implying that TOPK would be a rational target in developing novel anticancer drugs. Acetylshikonin, a diterpenoid compound isolated from Lithospermum erythrorhizon root, exerts a range of biological activities. Here we have investigated whether acetylshikonin, by acting as an inhibitor of TOPK, can attenuate the proliferation of colorectal cancer cells and the growth of patient-derived tumours, in vitro and in vivo. Experimental Approach: Targets of acetylshikonin, were identified using kinase profiling analysis, kinetic/binding assay, and computational docking analysis and knockdown techniques. Effects of acetylshikonin on colorectal cancer growth and the underlying mechanisms were evaluated in cell proliferation assays, propidium iodide and annexin-V staining analyses and western blots. Patient-derived tumour xenografts in mice (PDX) and immunohistochemistry were used to assess anti-tumour effects of acetylshikonin. Key Results: Acetylshikonin directly inhibited TOPK activity, interacting with the ATP-binding pocket of TOPK. Acetylshikonin suppressed cell proliferation by inducing cell cycle arrest at the G1 phase, stimulated apoptosis, and increased the expression of apoptotic biomarkers in colorectal cancer cell lines. Mechanistically, Abbreviations: PDX, patient-derived tumour xenograft; RSK, ribosomal s6 kinase; TOPK, T (T-LAK)-cell-originated protein kinase.Ran Zhao, Bu Young Choi, Lixiao Wei, and Mangaladoss Fredimoses equally contributed in this study.

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“…Three different concentrations of extracts (25, 50 and 100 μg/mL, chosen starting dilutions from the highest concentration of the value 1/10 lower than the concentration used to evaluate the eventual genotoxic effect, 1000 μg/mL) were pre-incubated for 2 h at 37˚C with known genotoxins, 4-NQO and 2-AA in the absence and presence of metabolic PLOS ONE activation S9, respectively. The percentage of antimutagenicity was calculated [31]:…”

Section: Umu Testmentioning

confidence: 99%

Antimutagenic, antigenotoxic and antiproliferative activities of Fraxinus angustifolia Vahl. leaves and stem bark extracts and their phytochemical composition

et al. 2020

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In recent years, chronic degenerative diseases such as certain types of cancers, are becoming an evident issue. DNA damage has been for long recognized as a causal factor for cancer development because mutations or chromosomal aberrations affect oncogenes and tumor suppressor genes leading cells to malignant transformation and to the subsequent cancerous growth. Medicinal plants are often used for the prevention or treatment of various diseases with great scientific interest. Among the medicinal plants distributed in the Mediterranean region, Fraxinus angustifolia Vahl. has been used in traditional medicine for its remarkable curative properties. However, in spite of this popularity, little works have been performed on the activity so that further studies should be performed to investigate in depth the antimutagenic, antigenotoxic and antiproliferative activities of the plant. Thus, the present study was aimed to the evaluation of the potential antimutagenic, antigenotoxic and antiproliferative properties of leaves and stem bark extracts of this well-known tree. Antimutagenic activity was evaluated by Salmonella mutagenicity assay in Salmonella typhimurium TA98 and TA100 strains. The antigenotoxic potential was assessed by umu test in the strain of S. typhimurium TA1535/pSK1002. Antiproliferative activity was studied on human hepatoblastoma (HepG-2) and on breast adenocarcinoma (MCF-7) cell lines by MTT assay. Furthermore, the antiproliferative activity observed on cancer cells was compared with that on the human normal-like fibroblasts (TelCOFS02MA) and the selectivity index was calculated to understand if extracts were able to exert selective toxicity towards cancer cells. Moreover, phenolic compounds are plant substances with a large spectrum of biochemical activities with antioxidant, antimutagenic and anticarcinogenic effects. Based on the strong evidence of biological activities of phenolic compounds, the study was focused on the determination of total phenolics and flavonoids contents, and the phytochemical composition of the extracts assessed by LC/MS. The ethanol extracts of both leaves and stem barks showed

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Antigenotoxic, anti-photogenotoxic and antioxidant activities of natural naphthoquinone shikonin and acetylshikonin and Arnebia euchroma callus extracts evaluated by the umu-test and EPR method

Cited by 32 publications

References 31 publications

Antigenotoxic, Anti-photogenotoxic, and Antioxidant Properties of Polyscias filicifolia Shoots Cultivated In Vitro

Antigenotoxic, Anti-photogenotoxic, and Antioxidant Properties of Polyscias filicifolia Shoots Cultivated In Vitro

Acetylshikonin suppressed growth of colorectal tumour tissue and cells by inhibiting the intracellular kinase, T‐lymphokine‐activated killer cell‐originated protein kinase.

Antimutagenic, antigenotoxic and antiproliferative activities of Fraxinus angustifolia Vahl. leaves and stem bark extracts and their phytochemical composition

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