Antigenotoxic and antioxidant activity in human chronic myelogenous leukaemia cell line K562 enhanced byNitraria retusaleaf extracts

Abstract

Nitraria retusa leaf extracts have been investigated for their ability to induce antioxidant and antigenotoxic effects in a human chronic myelogenous leukaemia cell line. Antioxidant and antigenotoxic properties of N. retusa products were explored using antioxidant and the assays, respectively. Hex, Chl and MeOH extracts decreased oxidation induced by 2,2 0 -azobis (2-amidinopropane) dihydrochloride in human cells, with IC 50 concentrations of 0.6, 0.52 and 0.24 mg/mL, respectively, reflecting significant antioxidant potential. The same products inhibited the genotoxicity induced by hydroxyl radicals in the same human cell line, by 67% at 600 mg/mL, 74% at 780 mg/mL and 81.5% at 800 mg/mL, respectively.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. Ã P < 0.05 compared to negative control without N. retusa extracts. Results are represented by the means AE SD of three experiments. J. Boubaker et al.

Assessment of the genotoxic and antigenotoxic potential of crude extracts and fractions of Schwartzia brasiliensis (Choisy) Bedell ex Giraldo-Caas

“…This protective effect has already been observed elsewhere, using the same experimental approach, with extracts of Cleome rosea (Simões et al, 2006), Daphne gnidium (Chaabane et al, 2012) and Nitraria retusa (Boubaker et al, 2013). The chromatograms obtained from the methanol extract and aqueous fraction of stems, using the method proposed, were insufficient to determine their constituents accurately.…”

“…The antigenotoxic effect of S. brasiliensis leaves may be related to their chemical constituents. Flavonoids are described as having antioxidant (Brunetti et al, 2013;Procházková et al, 2011;Romano et al, 2013) and antigenotoxic activities (Boubaker et al, 2013;Chaabane et al, 2012). In addition, flavonoids have antineoplastic effects and can also protect against cardiovascular and neurodegenerative diseases (Obrenovich et al, 2010;Obrenovich et al, 2011;Simões et al, 2006).…”