Antigenicity to neuropeptide F (NPF) in Stenostomum leucops and Microstomum lineare

Abstract

The first `native' flatworm regulatory peptide, neuropeptide F (NPF) has recently been isolated and sequenced from the cestode Moniezia expansa (see Maule et al., 1991) and the turbellarian Artioposthia triangulata, (see Curry et al., 1992) . NPF belongs to the neuropeptide Y (NPY) superfamily and the antiserum is known to show crossreactivity to the vertebrate neuropeptides of the NPY superfamily. It terminates in RFamide, like the invertebrate neuropeptides FMRFamide and RFamide, and may cross-react with neuropeptides of the FMRFamide family . Strong immunoreactivity (IR) to FMRF-and RF-amide has been demonstrated in members of most flatworm groups . In the present study, IR to NPF (diluted 1 :1000) is demonstrated in Stenostomum leucops (Catenulida) and Microstomum lineare (Macrostomida) . The controls included : omitting primary antibody, using non-immune serum and liquid-phase absorption with the homologous antigen (1000 ng ml -1 ) . The NPF IR pattern was compared to the FMRF and RF-amide IR patterns in order to reveal differences or co-localization . In addition, the sequential appearance of NPF-positive cells in developing zooids was followed and double staining with a-5-HT made to complete the study.

A taxonomic revision of South American species of the genus Stenostomum O. Schmidt (Platyhelminthes: Catenulida) based on morphological characters

“…In the 1990s, Reuter (Reuter, 1988, 1991, 1994; Reuter & Palmberg, 1990; Reuter & Eriksson, 1991; Reuter & Kuusisto, 1992; Reuter, Joffe & Palmberg, 1993; Reuter et al ., 1995) as well as Wikgren & Thorndyke (1990), Lindroos & Reuter (1991), Palmberg & Reuter (1992) and Grahn et al . (1995) carried out ultrastructural studies of stenostomids, mainly S. leucops .…”

The pattern of neuropeptide F and RF-amide in two tapeworms

“…Both free-living and parasitic flatworms are being studied . So far NPF-immunoreactivity has been reported from three free-living flatworms (see Grahn et al, 1995) and from four parasitic flatworms (Marks et al, 1993) .FMRF-and RF-amide immunoreactive (IR) nerve cells and fibres are common in the gull-tapeworm Diphyllobothrium dendriticum . In order to test whether the patterns for NPF-and RF-immunoreactivity co-localize in the gull-tapeworm, immunostaining with anti-NPF and anti-RF were performed .…”

“…Both free-living and parasitic flatworms are being studied . So far NPF-immunoreactivity has been reported from three free-living flatworms (see Grahn et al, 1995) and from four parasitic flatworms (Marks et al, 1993) .…”

Biodiversidad de turbelarios (Platyhelminthes) de la cuenca del Plata

“…Otros autores determinan ejemplares africanos como S. leucops (según la definición de Luther (1960)) y atribuyen las diferencias encontradas por Borkott (1970) a originadas en los cultivos y no visibles en los animales provenientes de ambientes naturales. Debido a que los ejemplares encontrados por Noreña-Janssen (1995b) en el río Paraná, Argentina, y los estudiados en este trabajo concuerdan con la diagnosis de , y que debido al amplio uso del nombre de esta especie ha quedado definido un "concepto" de lo que es la especie (Reuter, 1988(Reuter, , 1991(Reuter, , 1994Reuter & Palmberg, 1990;Reuter & Eriksson, 1991;Reuter & Kuusisto, 1992;Reuter et al, 1993Reuter et al, , 1995Wikgren & Thorndyke, 1990;Lindroos & Reuter, 1991;Palmberg & Reuter, 1992;Grahn et al, 1995;Noreña et al, aceptado), se mantiene la denominación de S. leucops.…”