Antigenicity of Polyribonucleotides<sup>*</sup>

Seaman, Edna; Vunakis, Helen Van; Levine, Lawrence

doi:10.1021/bi00883a015

Cited by 40 publications

(20 citation statements)

References 19 publications

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“…The progress already made is a reflection of a good understanding of the fundamentals of UV photochemistry which allows for control of,photoproduct formation in DNA and ease of analysis (17,18). Consequently, in the ensuing discussions, removal of the primary UV photoproducts, pyrimidine-pyrimidine dimers, is emphasized.…”

Section: Classification Of Nucleasesmentioning

confidence: 99%

Enzymatic Repair of DNA

Grossman¹,

Braun²,

Feldberg³

et al. 1975

Annu. Rev. Biochem.

203

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Section: Classification Of Nucleasesmentioning

confidence: 99%

Enzymatic Repair of DNA

Grossman¹,

Braun²,

Feldberg³

et al. 1975

Annu. Rev. Biochem.

203

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“…Each of these antibodies is characteristically found at high levels in the sera of patients with systemic lupus erythematosus (SLE), and except for anti-NDNA antibodies, they also have been observed in the sera of patients with a variety of diseases associated with active tissue destruction (8). Experimental studies indicate that an appropriate immunogen may induce antibodies to SDNA (13,14), DSRNA (15), polyribonucleotides (16,17), and ribosomes (18), but not to NDNA. Anti-NDNA antibodies are found only in the spontaneous NZB/W disease of mice (19,20).…”

mentioning

confidence: 99%

Antibodies to Polynucleotides in Human Sera: Antigenic Specificity and Relation to Disease

Koffler

Carr

Agnello

et al. 1971

The Journal of Experimental Medicine

464

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The specificities of anti-polynucleotide antibodies found in human sera were studied using several immunological procedures. Anti-native DNA (NDNA) antibodies and certain anti-double-stranded RNA (DSRNA) antibodies were found to react with single-stranded DNA (SDNA), and anti-NDNA antibodies were observed to react more avidly with SDNA than with NDNA in most sera tested. Antibodies to NDNA showed no preferential reactivity with NDNA or SDNA derived from mammalian tissue, bacterial, or viral sources. Precipitating antibodies reactive with individual bases, with common determinants of bases, and with common determinants of SDNA and NDNA were detected utilizing synthetic polydeoxyribonucleotides. Antibodies to DSRNA were also heterogeneous and reactive with both Poly A · Poly U and Poly I · Poly C in addition to reactivity with Poly A and SDNA. In contrast, antibodies to a ribonucleo-protein determined by hemagglutination and by precipitation showed no reaction with NDNA, SDNA, or DSRNA. Serial studies of serum specimens from patients with systemic lupus erythematosus (SLE) indicated that anti-NDNA antibodies were closely associated with disease activity. Titers of antibodies to SDNA or DSRNA were also frequently increased during these periods but in addition showed peaks during quiescent periods. Anti-NDNA antibodies were detected in most patients' sera at sometime during the course of the disease. Three patients were observed with active SLE, who did not develop anti-NDNA antibodies, even in the presence of severe renal disease. Evidence that other antigen-antibody systems may also play a role in the pathogenesis of the renal disease was particularly apparent in these patients. Anti-ribonucleoprotein antibodies were not well correlated with the peaks of antibody activity of other polynucleotide antibodies, suggesting that an independent immunogen was responsible for induction of these antibodies. The close association of certain populations of anti-polynucleotide antibodies during the course of active SLE, the presence of cross-reacting antigenic determinants of SDNA, NDNA, and DSRNA, the preferential avidity of anti-NDNA antibodies for SDNA, and the frequent increase of anti-SDNA antibodies in SLE and other diseases associated with active tissue destruction suggest that SDNA is a ubiquitous antigen that may stimulate the formation of antibodies reactive with a variety of polynucleotides.

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“…We therefore proposed that the injected, immunogenic RNA polymerase I had become associated with the DNA present in blood plasma (15), which led to induction of an immune response against the normally nonimmuogenic (16) nucleic acid component of the complex. The immunogenic property of nucleic acids when they are complexed with protein has been well established (17). Because antibodies against RNA polymerase I and DNA are both characteristic of SLE (1-3), we suggested (14) that a similar mechanism may be involved in the induction ofanti-DNA antibody formation in rheumatic disease.…”

Section: Discussionmentioning

confidence: 96%

Anti-RNA polymerase I antibodies in sera of MRL lpr/lpr and MRL +/+ autoimmune mice. Correlation of antibody production with delayed onset of lupus-like disease in MRL +/+ mice.

Stetler¹,

Sipes²,

Jacob³

1985

The Journal of Experimental Medicine

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Sera from individual MRL/lpr and MRL/++ mice, which develop an autoimmune disease similar to human systemic lupus erythematosus (SLE), were screened over a period of approximately 30 wk for the presence of anti-RNA polymerase I and anti-ssDNA antibodies. Even though onset of the disease is delayed in MRL/++ as compared to MRL/lpr mice, anti-ssDNA antibodies were present in comparable concentrations in the sera of all mice by the age of 6 wk. As observed in sera of human SLE patients, anti-RNA polymerase I antibodies were detected in the sera of all MRL mice. However, unlike the anti-ssDNA antibodies, anti-RNA polymerase I antibodies were detected much later in MRL/++ mice (mean age, 22.8 wk) as compared to MRL/lpr mice (mean age, 9.6 wk). The presence of anti-RNA polymerase I antibodies in sera of MRL mice was thus a much better indicator of disease status than the presence of anti-ssDNA antibodies. The appearance and increase in anti-RNA polymerase I antibodies in the sera of MRL/++ mice correlated (R2 = 0.964) with a precipitous decrease in anti-ssDNA antibodies, starting at about 20 wk of age. These results suggest a possible relationship between the RNA polymerase I and DNA autoimmune reactions.

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Antigenicity of Polyribonucleotides^*

Cited by 40 publications

References 19 publications

Enzymatic Repair of DNA

Enzymatic Repair of DNA

Antibodies to Polynucleotides in Human Sera: Antigenic Specificity and Relation to Disease

Anti-RNA polymerase I antibodies in sera of MRL lpr/lpr and MRL +/+ autoimmune mice. Correlation of antibody production with delayed onset of lupus-like disease in MRL +/+ mice.

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Antigenicity of Polyribonucleotides*

Cited by 40 publications

References 19 publications

Enzymatic Repair of DNA

Enzymatic Repair of DNA

Antibodies to Polynucleotides in Human Sera: Antigenic Specificity and Relation to Disease

Anti-RNA polymerase I antibodies in sera of MRL lpr/lpr and MRL +/+ autoimmune mice. Correlation of antibody production with delayed onset of lupus-like disease in MRL +/+ mice.

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Product

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Antigenicity of Polyribonucleotides^*