Antigenic Variation in Malaria

Brown, K. N.

doi:10.1007/978-1-4615-8855-9_2

Cited by 10 publications

(3 citation statements)

References 28 publications

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“…Further, the variant specificity was not altered by adsorption with enzyme-treated erythrocytes. 4. Demonstration of antibody binding to the surface schizont-infected erythrocytes and merozoites of variant PK1.…”

Section: Resultsmentioning

confidence: 99%

“…This surface coat is morphologically similar to the coat observed on the surface of Trypanosoma brucei, which has been shown to be a single glycoprotein that undergoes antigenic variation (1, 31). The morphological similarity of the surface coat and the existence of an antigenic variation in P. knowlesi infections suggested the hypothesis that the surface coat of merozoites was, as in trypanosomes, the variant antigen and was therefore related to the antigen detected on the schizont-infected erythrocyte (4). The existence of a variant antigen covering the surface of merozoites could be an important issue in the development of a malaria vaccine using purified merozoites (25,26).…”

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confidence: 99%

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Plasmodium knowlesi variant antigens are found on schizont-infected erythrocytes but not on merozoites

Hommel¹,

David²

1981

Infect Immun

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In this report, we demonstrate that the variable antigens present on the surface of Plasmodium knowlesi-infected erythrocytes could not be found on the surface of merozoites. A number of technical problems had to be solved to make such a comparative study possible, including the purification of merozoites by affinity columns and gradient centrifugation, the use of hyperimmune rabbit sera instead of monkey sera, and the use of immunocytological methods (indirect immunofluorescence antibody test and electron microscopy with ferritin-labeled antibodies) instead of the schizont-infected cell agglutination test. To ensure that these new techniques were valid for variant-specific serotyping, we compared two wellcharacterized variant populations with both the standard and the new methods. The removal of variant antigens from the surface of infected erythrocytes by proteolytic enzymes provides further information on the biochemical nature of these antigens.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

Plasmodium knowlesi variant antigens are found on schizont-infected erythrocytes but not on merozoites

Hommel¹,

David²

1981

Infect Immun

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“…The SICA antigen as described by Brown (1977), was variant specific and had a patchy distribution on the surface of the unfixed red cell when examined by immunoelectron microscopy (Brown & Hockley, 1966), or by indirect immunofluorescence (IFAT). IFAT on fixed cells showed no variant specificity presumably because of strong fluorescence of common internal antigens.…”

Section: Schizont-infected Cell Agglutinin (Sica)mentioning

confidence: 99%

Membrane structure and function of malaria parasites and the infected erythrocyte

Sherman

1985

Parasitology

106

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C T I V E S is a series of articles which are intended to: 1. Focus attention on developing and growing topics in parasitology. 2. Identify new and old problems in need of research. 3. Explain how results from one area of biology might have some bearing on current studies in parasitology. 4. Present teachers and students with concise summaries of recent discoveries and new theories. None of the articles will take the form of a detailed historical survey of the literature. In seeking contributions for the series, the editors have urged authors to present clear and stimulating personal points of view about those topics which have excited their curiosity and imagination. The editors hope that a wide readership will gain enjoyment and benefit from this enterprise. Parasitology (1985), 91, 609-645 6 0 9 With 3 figures in the text

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