Antigenic validation of recombinant hemagglutinin-neuraminidase protein of Newcastle disease virus expressed in Saccharomyces cerevisiae

Sa, Khulape; Maity, Hemanta Kumar; Dc, Pathak; Cm, Mohan; Dey, Sutirth

doi:10.4149/av_2015_03_240

Cited by 10 publications

(2 citation statements)

References 14 publications

(17 reference statements)

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“…Using pESC-URA plasmid, SDH2-R32C and SNCA genes (synthesized by Kingsley Biotechnology Co., Ltd., China) were exogenously inserted and introduced into yeast cells for expression ( Khulape et al, 2015 ).…”

Section: Methodsmentioning

confidence: 99%

Unraveling the functional consequences of a novel germline missense mutation (R38C) in the yeast model of succinate dehydrogenase subunit B: insights into neurodegenerative disorders

Zheng,

Liu,

Wang

et al. 2023

Front. Mol. Neurosci.

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This study explores the implications of a novel germline missense mutation (R38C) in the succinate dehydrogenase (SDH) subunit B, which has been linked to neurodegenerative diseases. The mutation was identified from the SDH mutation database and corresponds to the SDH2R32C allele, mirroring the human SDHBR38C mutation. By subjecting the mutant yeast model to hydrogen peroxide (H2O2) stress, simulating oxidative stress, we observed heightened sensitivity to oxidative conditions. Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) analysis revealed significant regulation (p < 0.05) of genes associated with antioxidant systems and energy metabolism. Through gas chromatography-mass spectrometry (GC-MS) analysis, we examined yeast cell metabolites under oxidative stress, uncovering insights into the potential protective role of o-vanillin. This study elucidates the biological mechanisms underlying cellular oxidative stress responses, offering valuable insights into its repercussions. These findings shed light on innovative avenues for addressing neurodegenerative diseases, potentially revolutionizing therapeutic strategies.

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Section: Methodsmentioning

confidence: 99%

Unraveling the functional consequences of a novel germline missense mutation (R38C) in the yeast model of succinate dehydrogenase subunit B: insights into neurodegenerative disorders

Zheng,

Liu,

Wang

et al. 2023

Front. Mol. Neurosci.

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“…with Salmonella flagellin as adjuvant. Khulape et al (2015) attempted to express the HN protein of NDV in a yeast expression system. The authors found that Saccharomyces cerevisiae was a better expression system for HN protein than Pichia pastoris as determined by codon usage analysis.…”

Section: Introductionmentioning

confidence: 99%

Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus

et al. 2017

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Newcastle disease virus (NDV) is one of the most important viral pathogens of avian species and the causative agent of atypical fowl plague, a highly contagious and economically important disease characterized by high mortality rates and reduction of egg production. The HN and F proteins are the main targets for immune response to NDV. Vaccination of poultry with live and inactivated NDV vaccines is the most effective method of control and prevention of Newcastle disease, however due to their disadvantages, efforts are being invested into developing subunit vaccines. To this end, the NDV HN and/or F protein have been expressed using different viruses as vectors, but have also been expressed using transgenic plant systems, yeast and lactic acid bacteria in order to produce the NDV subunit vaccine. Many authors have investigated the possibility of preparation of vaccines from purified and biologically active NDV subunits with HN and F glycoproteins, purified from nucleocapsids, viral ribonucleic acid (RNA) and pyrogens. The above mentioned viral glycoproteins with preserved antigenic structure and biological activities can be used as subunit vaccinal antigens due to their immunogenic properties.

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Contribution of yeast models to virus research

Glingston

Yadav

Rajpoot

et al. 2021

Appl Microbiol Biotechnol

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Time and again, yeast has proven to be a vital model system to understand various crucial basic biology questions. Studies related to viruses are no exception to this. This simple eukaryotic organism is an invaluable model for studying fundamental cellular processes altered in the host cell due to viral infection or expression of viral proteins. Mechanisms of infection of several RNA and relatively few DNA viruses have been studied in yeast to date. Yeast is used for studying several aspects related to the replication of a virus, such as localization of viral proteins, interaction with host proteins, cellular effects on the host, etc. The development of novel techniques based on high-throughput analysis of libraries, availability of toolboxes for genetic manipulation, and a compact genome makes yeast a good choice for such studies. In this review, we provide an overview of the studies that have used yeast as a model system and have advanced our understanding of several important viruses. Key points• Yeast, a simple eukaryote, is an important model organism for studies related to viruses.• Several aspects of both DNA and RNA viruses of plants and animals are investigated using the yeast model.• Apart from the insights obtained on virus biology, yeast is also extensively used for antiviral development.

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Antigenic validation of recombinant hemagglutinin-neuraminidase protein of Newcastle disease virus expressed in Saccharomyces cerevisiae

Cited by 10 publications

References 14 publications

Unraveling the functional consequences of a novel germline missense mutation (R38C) in the yeast model of succinate dehydrogenase subunit B: insights into neurodegenerative disorders

Unraveling the functional consequences of a novel germline missense mutation (R38C) in the yeast model of succinate dehydrogenase subunit B: insights into neurodegenerative disorders

Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus

Contribution of yeast models to virus research

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