The UspA2 protein has been shown to be directly involved in the serum-resistant phenotype of Moraxella catarrhalis. The predicted 5-untranslated regions (UTR) of the uspA2 genes in several different M. catarrhalis strains were shown to contain various numbers (i.e., 6 to 23) of a heteropolymeric tetranucleotide (AGAT) repeat. Deletion of the AGAT repeats from the uspA2 genes in the serum-resistant M. catarrhalis strains O35E and O12E resulted in a drastic reduction in UspA2 protein expression and serum resistance. PCR and transformation were used to construct a series of M. catarrhalis O12E strains that differed only in the number of AGAT repeats in their uspA2 genes. Expression of UspA2 was maximal in the presence of 18 AGAT repeats, although serum resistance attained wild-type levels in the presence of as few as nine AGAT repeats. Increased UspA2 expression was correlated with both increased binding of vitronectin and decreased binding of polymerized C9. Real-time reverse transcription-PCR analysis showed that changes in the number of AGAT repeats affected the levels of uspA2 mRNA, with 15 to 18 AGAT repeats yielding maximal levels. Primer extension analysis indicated that these AGAT repeats were contained in the 5-UTR of the uspA2 gene. The mRNA transcribed from a uspA2 gene containing 18 AGAT repeats was found to have a longer half-life than that transcribed from a uspA2 gene lacking AGAT repeats. These data confirm that the presence of the AGAT repeats in the 5-UTR of the uspA2 gene is necessary for both normal expression of the UspA2 protein and serum resistance.Among the bacteria that can colonize the human nasopharynx, three different organisms (Streptococcus pneumoniae, nontypeable Haemophilus influenzae, and Moraxella catarrhalis) are noted for their ability to cause otitis media in infants and very young children (15). M. catarrhalis, an unencapsulated, gram-negative coccobacillus previously termed either Neisseria catarrhalis or Branhamella catarrhalis (12), is the third most common cause of otitis media in the pediatric population (17). More recent clinical studies have reaffirmed the pathogenic potential of this bacterium in adults, indicating that M. catarrhalis may cause as many as four million exacerbations of chronic obstructive pulmonary disease annually in the United States (45).Although the ability of this organism to produce disease is now well established, information about the M. catarrhalis gene products essential for pathogenesis remains relatively limited. Among the various putative virulence factors that have been identified to date (reviewed in references 28, 42, and 53), several proteinaceous antigens have been shown to protrude from the outer membrane of M. catarrhalis. These include the UspA1 and UspA2 proteins (2, 25, 47), the Hag (MID) hemagglutinin (19, 47), and type IV pili (32). Interestingly, expression of both the UspA1 protein and the Hag protein appear to be subject to phase variation, although the relevant mechanisms are different. A homopolymeric nucleotide repeat conta...