Antigenic S-type lipopolysaccharide of Brucella abortus 1119-3

Abstract

Antigenic phenol-phase soluble lipopolysaccharide isolated from Brucella abortus 1119-3 by hot phenol-water extraction was shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, controlled hydrolysis, periodate oxidation, methylation, and 'H and 1 C nuclear magnetic resonance studies to be an S-type lipopolysaccharide which could be cleaved to yield a lipid A and an 0-chain polysaccharide identified as an unbranched linear homopolymer of 1,2-linked 4,6-dideoxy-4-formamido-ft-D-mannopyranosyl residues. The serological reactivity of bovine antiserum to B. abortus 1119-3 with the lipopolysaccharides of Yersinia enterocolitica serotype 0:9 and Vibrio cholerae species has now been related to the occurrence of 1,2-linked Nacylated 4-amino-4,6-dideoxy-a-D-mannopyranosyl units in the 0-chain polysaccharides of their lipopolysaccharides.The diagnosis of brucellosis in humans and animals is frequently difficult to establish. Often the infection either is subclinical or yields varied responses in the host. Diagnosis, therefore, has frequently been based on the detection and quantification of Brucella antibodies in serum samples by agglutination, precipitin, complement fixation, and other methods (11). More recently, enzyme immunoassay tests with crude or purified antigens have been introduced (4, 20-22). False-negative and nonspecific reactions may be problems in these test systems. This report describes our work on the isolation, purification, and determination of the structure of a unique Brucella surface antigen which could be used both for diagnostic tests and for studies involving the production and use of monoclonal antibodies for the identification of Brucella abortus.The present investigation led to the identification of the antigenic 0-chain polysaccharide of B. abortus S-type lipopolysaccharide (LPS) as a linear homopolymer of 1,2-linked 4,6-dideoxy-4-formamido-a-D-mannopyranosyl units.(This paper was presented previously

Characterization of Biological Activities of Brucella melitensis Lipopolysaccharide

“…Specifically, the lipid A moiety of B. abortus LPS possesses a diaminoglucose backbone, and the acyl groups are longer (C18-19, C28) and are only linked to the core by amide bounds (22,30). Further, the O antigen of Brucella LPS is a homopolymer of perosamine (4,6dideoxy-4-formamido-d-mannopyranosyl) (3,27,37). The biological activities such as cytokine production and lethality of S-LPS and rough types of LPS (R-LPS) from various Brucella strains are reported to be lower than those of enterobacterial LPS (4,14,25,29).…”

A review of enzyme immunoassay for detection of antibody to Brucella abortus in cattle

“…For BruceUa, it is the sLPS-complex which is involved in almost all serological tests (Diaz et al, 1968). Some controversy exists as to whether the sLPS of BruceUa is complexed to proteins (Perera et al, 1984) or can be isolated (Caroff et al, 1984a). Possibly both groups are correct or, depending on the preparation, the sLPS may be a population of molecules varying in the amount of covalently bound proteins.…”

Structure and serologic properties of O-specific polysaccharide from Citrobacter freundii possessing cross-reactivity with Escherichia coli O157:H7

“…Lipopolysaccharides (LPS) exhibit broad cross-reactivity of E. coli O157 with other Gram-negative bacteria, such as Escherichia hermannii [5], Salmonella sp. group N (O30) [6], Vibrio cholerae O1 [7], Yersinia enterocolitica O9 [8], Brucella abortus [9], Pseudomonas maltophilia 555 [10], and Citrobacter freundii [11]. The cross-reactivity is caused by O-speci¢c polysaccharide antigens in LPS, which contain similar epitopes involving N-acyl or N-acetyl derivatives of 4-amino-4,6-dideoxy-K-D-mannopyranosyl residues [5^10].…”