Antigenic relationships of murine coronaviruses: Analysis using monoclonal antibodies to JHM (MHV-4) virus

Abstract

Monoclonal antibodies were produced to JHMV-DL, a neurotropic member of the mouse hepatitis virus (MHV) or murine coronavirus group. Of 23 antibodies isolated, 10 were specific for the major envelope glycoprotein, gp180/90, 10 for the nucleocapsid protein, pp60, and 3 for the minor envelope glycoprotein, gp25. Eleven different MHV isolates were used in antibody binding assays to study antigenic relationships among the viruses. Each MHV isolate tested had a unique pattern of antibody binding, indicating that each is a distinct strain. Conservation of JHMV-DL antigenic determinants varied among the three proteins, with pp60 showing intermediate conservation, gp180/90 little conservation, and gp25 marked conservation in the different MHV strains. Monoclonal antibodies to pp60 proved most useful in delineating antigenic relationships among MHV strains. These antigenic groups correlated with pathogenic types, indicating that pp60 may be one of the gene products which mediates the distinct disease patterns manifested by different murine coronaviruses.

Antigenic and Polypeptide Structure of Turkey Enteric Coronaviruses as Defined by Monoclonal Antibodies

“…A comparative study of nine TGEV strains, using a panel of neutralizing MAbs that recognized four major antigenic sites and at least nine possible epitopes, distinguished two variants by seroneutralization, which could not be distinguished using conventional sera . Nevertheless, distinct antigenic strains as defined for murine coronaviruses and IBV, where each isolate has a unique pattern of reactivity with antiglycoprotein MAbs (Fleming et al, 1983), was not reported for TGEV. Four epitopes were defined on the haemagglutinin of BCV .…”

“…The peplomeric E2 glycoprotein is often cleaved post-translationally by host cell proteases into two 85K to 100K subunits (Sturman et al, 1985;Cavanagh et al, 1986b). The latter also is known to be responsible for virus attachment and cell membrane fusion (Collins et al, 1982), and elicits the production of virus-neutralizing antibodies (Fleming et al, 1983;Laude et al, 1986;Niesters et al, 1987; which may be protective (Buchmeier et al, 1984;Wege et al, 1984;Cavanagh et al, 1986a). An additional glycoprotein of 130K to 140K, a disulphide-linked dimer of 65K subunits, is associated with the haemagglutinin (E3) of viruses belonging to the subgroup of haemagglutinating mammalian coronaviruses (King et al, 1985 ;Hogue & Brian, 1986: Sugiyama et al, I986).…”

NKG2D Receptor Signaling Enhances Cytolytic Activity by Virus-Specific CD8+T Cells: Evidence for a Protective Role in Virus-Induced Encephalitis

“…revealed no differences in either the number or the distribution of viral antigen in spinal cords of mice treated with either rat IgG (A) (magnification, ϫ100) or anti-NKG2D (B) (magnification, ϫ100). Viral antigen was detected by immunoperoxidase staining by using anti-JHMV MAb J.3.3, and antigen-positive cells were demonstrated by the presence of chromogen (arrows) (28,90). Representative LFB-stained spinal cord sections from JHMV-infected cells at day 32 p.i.…”

“…Slides containing stained spinal cord sections were blinded and scored by three investigators, and scores were averaged and are presented as averages Ϯ standard errors of the means (SEM). The distribution of viral antigen was determined by immunoperoxidase staining (Vectastain-ABC kit; Vector Laboratories, Burlingame, CA) using the anti-JHMV MAb J.3.3 specific for the carboxyl terminus of the viral nucleocapsid protein as a primary antibody and horse anti-mouse IgG as the secondary antibody (Vector Laboratories, Burlingame, CA) (7,28,90). The data presented represent a minimum of six spinal cord sections per mouse with no fewer than three mice per time point.…”

Studies on the Development of an ELISA Kit for Microbiological Monitoring

“…It has been demonstrated that there are some MHV antigen strains with partially different antigen constructions [1] . Machii et al [5] investigated reactivties of four murine coronavirus antigens (SDAV, PCV, MHV-S and MHV-NuU) with rat antisera and demonstrated cross-reactivity of MHV-S and MHV-NuU antigens with SDAV and PCV antisera.…”