ANTIGENIC RELATIONSHIPS OF TORULOPSIS GLABRATA AND SEVEN SPECIES OF THE GENUS CANDIDA

Abstract

Torulopsis glabrata, like a number of other yeasts and yeastlike fungi commonly associated with the human body, has been suspected of causing disease. It is an occasional inhabitant of the oral, gastrointestinal, and urinary tracts and most isolates have been obtained from human sources. Wickerham (1957) has presented the identifying characteristics of this yeast, and suggested its recognition as a potential pathogen. Although Wickerham indicated that he had identified strains of T. glabrata isolated from blood cultures, references to these cases were not given.

Comparison of cytoplasmic extracts of eight Candida species and Saccharomyces cerevisiae

“…Hasenclever and co-workers demonstrated that surface antigenic determinants of C. albicans are expressed chiefly by cell wall mannan and divided C. albicans into two serogroups on the basis of partial A identity of their cell wall mannans. Work by these investigators (12)(13)(14)28) and Fraser (7) demonstrated cross-reactivity of surface antigens of C. albicans with all yeast species studied in the present report. We have confirmed this observation by demonstrating that cell wall extracts of all of these yeasts form precipitin lines when tested by double immunodiffusion against antiserum to heat-killed C. albicans (unpublished data).…”

Antigenic Studies of Candida

“…Antisera to two strains each of group A C. albicans, group B C. albicans, C. stellatoidea, and C. tropicalis were prepared as previously described (Hasenclever and Mitchell, 1960b). Reciprocal cross adsorptions of the antisera with each of the immunizing strains were done (Hasenclever and Mitchell, 1961).…”

Serotypes in Saccharomyces telluris: Their Relation to Source of Isolation

“…In only two species of yeasts have distinct antigenic serotypes or groups been demonstrated. Evans (1) reported antigenic serotypes based on capsular polysaccharides in Cryptococcus neoformans, and antigenic groups have been observed also in Candida albicans (2). Recently, Saccharomyces telluris was found to be a prominent member of the normal yeast biota of columbid (pigeons and doves) species (4).…”

“…Tube agglutination tests were used to show the antigenic diversity that existed among the strains studied. The method utilized was described previously (2).…”

“…Usually, an additional series of 6 to 10 injections given on the same schedule was required to produce the required titers. Reciprocal cross absorption of all antisera with the immunizing strains was doile after a procedure described previously (2). Agglutination tests were then performed.…”