Antigenic Relationships Among Some Candida Species Studied by Crossed-Line Immunoelectrophoresis: Taxonomic Significance

Gabriel-Bruneau, Sylvie M.; Guinet, R

doi:10.1099/00207713-34-2-227

Cited by 24 publications

(18 citation statements)

References 39 publications

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“…One gel was 1.5 mm thick and contained holes (diameter, 2.5 mm) that were 10 mm apart for the antigenic extracts that were to be compared; the second gel was 1.3 mm thick and contained the antigenic extract used as a reference; and the third gel was 1 mm thick and contained the antiserum that was homologous to the reference antigen. The optimal antigen-antibody ratio was determined in preliminary line immunoelectrophoresis experiments (6). The concentrations of antigens and antisera in the last two gels varied from 5 to 20% (vol/vol).…”

Section: Methodsmentioning

confidence: 99%

Antigenic Relationships among Bacteriodes Species Studied by Rocket-Line Immunoelectrophoresis

Tessier

Quentin

Capdepuy

et al. 1993

International Journal of Systematic Bacteriology

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Antigenic relationships among the 10 Bacteroides species, Porphyromonas gingivalis, Prevotella bivia, and Prevotella intermedia were investigated by rocket-line immunoelectrophoresis, using antigenic extracts prepared from type strains and antisera to Bacteroides fragilis, Bacteroides distasonis, Bacteroides thetaiotaomicron, Porphyromonas gingivalis, and Prevotella intermedia. Among the members of the genus Bacteroides, B. fragdis, Bacteroides caccae, Bacteroides stercoris, Bacteroides vulgatus, B. thetaiotaomicron, and Bacteroides ovatus were found to be closely related, and B. distasonis and Bacteroides merdue were the species that were most distantly related to B. fragilis; Bacteroides eggerthii and Bacteroides uniformis were in an intermediate position. Porphyromonas gingivalis, Prevotella bivia, and Prevotella intermedia shared very low percentages of common antigens with the Bacteroides species. Detection of enzymic activities in immunoprecipitates allowed us to identify some antigens to enzymes and to study the immunological similarities of the enzymes. The malate dehydrogenases of the Bacteroides species were genus specific, and the glucose-6-phosphate dehydrogenases of most species exhibited antigenic similarities, The esterases of B. distasonis and B. thehiohomicron appeared to be species specific, whereas the esterases and phosphatases of B. fragilis cross-reacted with the esterases and phosphatases of B. stercoris and B. vulgatus, as did the phosphatases of B. distasonis and B. merdae. The phosphatases of B. eggerthii, B. uniformis, Porphyromonas gingivalis, and Prevotella intermedia were antigenically similar.Organisms belonging to the genus Bacteroides Castellani and Chalmers 1919 are obligately anaerobic, gram-negative, nonsporing, rod-shaped bacteria (7, 8). However, this genus is known to be phenotypically and phylogenetically extremely heterogeneous. Thus, the 39 species included in the genus in Betgey's Manual of Systematic Bacteriology (7) exhibit a variety of cellular morphologies and saccharolytic and proteolytic activities and have DNA base compositions ranging from 28 to 61 mol% guanine plus cytosine.On the basis of biochemical, chemical, and molecular biological criteria (10,18,20), the majority of the more typical Bacteroides species form three broad groups. The first group consists of Bacteroides frasilis and related saccharolytic intestinal species. This group includes five species which at one time were described as subspecies of a single species, B. fragdis (8) Shah and Collins (20) have proposed that the genus Bacteroides should be restricted to the 10 species belonging to the B. fragiis group and that the members of the second and third groups should be transferred to the new genera Prevotella and Porphyromonas,

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Section: Methodsmentioning

confidence: 99%

Antigenic Relationships among Bacteriodes Species Studied by Rocket-Line Immunoelectrophoresis

Tessier

Quentin

Capdepuy

et al. 1993

International Journal of Systematic Bacteriology

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“…Antisera were obtained by immunizing rabbits Immunizations, as well as evaluations of the titers of serum antibodies, were performed by previously described methods (7).…”

Section: )mentioning

confidence: 99%

Antigens and Enzymes of Bacteroides of the Bacteroides fragilis Group Compared by Crossed Immunoelectrophoresis

Rogemond

Guinet

1985

International Journal of Systematic Bacteriology

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In this work we were primarily concerned with antigens of Bacteroides strains belonging to the B. fragilis group. The following two antisera were produced: anti-Bacteroides fragilis ElT (T = type strain) and anti-Bacteroides distasonis. Reference patterns for both of these species were established by crossed immunoelectrophoresis, and these patterns yielded 62 and 70 precipitates, respectively. The crossed reactions of B. fragilis ElT and B . distasonis with Bacteroides fragilis E2, Bacteroides thetaiotaomicron, Bacteroides ovatus and Bacteroides vulgatus were studied to test both for total antigens and for certain enzymes (esterase, phosphatase, malate dehydrogenase, and glucose-6-phosphate dehydrogenase). Our results showed that B. thetaiotaomicron is the species which is closest to B. fragilis, demonstrating more than 70% common antigens, whereas none of the species examined was very closely related to B. distasonis. Species-specific antigens, such as the phosphatase of B. fragilis, as well as antigens common to the group, such as malate dehydrogenase, were also demonstrated in crossed immunoelectrophoresis. This may allow immunological identification of each species.Anaerobic bacteria are responsible for at least 10% of all bacterial infections, and Bacteroides species alone represent 27% of the anaerobes isolated from pathological materials (22). Bacteroides strains belonging to the B. fragilis group are most often responsible for intestinal infections (6, 27). The most frequently isolated species is Bacteroides fragilis, although this species is a minor component of the fecal flora, in which Bacteroides vulgatus and Bacteroides thetaiotaomicron are the dominant species (18). This observation has led some authors to seek virulence factors in B . fragilis (notably in the capsule) that are lacking in the other species of the group (13, 19).Classification of the B . fragilis group has been modified often. The numerous phenotypic properties which members of this group have in common account for the proposal of subspecies of B . fragilis (17). However, deoxyribonucleic acid-deoxyribonucleic acid homology studies showed a diversity (3) which led to reclassification of B . fragilis, Bacteroides ovatus, B. vulgatus, B . thetaiotaomicron, and Bacteroides distasonis as distinct species (12).In 1971, Beerens et al. (1) proposed a serological classification for the B. fragilis group, which was based on agglutination or gel double-diffusion tests in the presence of a specific antiserum. Lambe and Moroz (15) tested for the presence of thermostable antigens that permitted classification of all B . fragilis strains without cross-reactions with B . distasonis, B . ovatus, and B . thetaiotaomicron. Other authors have studied the lipopolysaccharide fractions or outer membrane complex of B. fragilis by using either sodium dodecyl sulfate-polyacrylamide gel electrophoresis (4, 14, 21) or immunochemical techniques (5, 10).All of these studies demonstrated the heterogeneity which exists within the B . fragilis group, as well as t...

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“…Other methods of identification have been described. The electrophoretic or antigenic properties of yeast enzymes allowed good species discrimination but the preparation of extracts was time consuming [9,10]. The electrophoretic or antigenic properties of yeast enzymes allowed good species discrimination but the preparation of extracts was time consuming [9,10].…”

Section: Introductionmentioning

confidence: 99%

“…Agglutination tests with species-specific antisera were limited by the lack of specificity of some sera especially for the species C. albicans and C. tropicalis [8]. The electrophoretic or antigenic properties of yeast enzymes allowed good species discrimination but the preparation of extracts was time consuming [9,10]. Monoclonal antibodies able to agglutinate blastospores were purified but again were not speciesspecific [11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Rapid identification of medically important yeasts by electrophoretic protein patterns

Bruneau

1989

FEMS Microbiology Letters

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A simple electrophoretic method for yeast identification was evaluated. Whole cells were extracted by SDS and the protein profiles obtained in SDS-PAGE after Coomassie blue staining were compared for 52 strains from 9 species of yeast or yeast-like fungi commonly isolated from man (Candida albicans, C. glabrata, C. guilliermondii, C. krusei, C. parapsilosis, C. pseudotropicalis, C. tropicalis, Geotrichum candidum, Saccharomyces cerevisiae). The corresponding patterns showed 30 to 45 polypeptides in the range 95-20 kDa and were clearly different for the 9 species. No differences could be detected between strains from the same species. The characteristic patterns were obtained within 24 h allowing rapid identification of the most commonly encountered clinical yeast isolates.

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Antigenic Relationships Among Some Candida Species Studied by Crossed-Line Immunoelectrophoresis: Taxonomic Significance

Cited by 24 publications

References 39 publications

Antigenic Relationships among Bacteriodes Species Studied by Rocket-Line Immunoelectrophoresis

Antigenic Relationships among Bacteriodes Species Studied by Rocket-Line Immunoelectrophoresis

Antigens and Enzymes of Bacteroides of the Bacteroides fragilis Group Compared by Crossed Immunoelectrophoresis

Rapid identification of medically important yeasts by electrophoretic protein patterns

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