Antigenic Relationship of Varicella-Zoster and Herpes Simplex

Ross, Constance A. C.; Sharpe, J H Subak; Ferry, Peggy C.

doi:10.1016/s0140-6736(65)90452-6

Cited by 71 publications

(28 citation statements)

References 13 publications

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“…One case who had had chickenpox but not zoster had a high level of neutralizing antibody and the possible reasons for this are discussed. No cross-neutralization with Herpes simplex virus was demonstrated but the rise in titre of complement-fixing antibody to HS occurring in herpetic subjects with chickenpox (Ross et al 1965) was confirmed. Two samples of human gamma-globulin were shown to have high levels of neutralizing antibody to V-Z virus and one was known to have been found effective clinically.…”

Section: Discussionmentioning

confidence: 88%

“…If the high level of antibody in case 12 is the result of reinfection by contact with chickenpox or zoster or of recrudesence of latent virus then either of these events must have occurred sufficiently long ago for the CF antibody to have waned although the neutralizing antibody persists. Ross et al (1965) showed a rise in CF antibody to HS in 48 % of their chickenpox patients and in 26 % of their zoster patients. They also showed slight rises (less than fourfold) in neutralizing antibody to HS in some chickenpox cases.…”

Section: Discussionmentioning

confidence: 99%

“…Neutralization tests were carried out by the method described by Ross et al (1965) using the plaque assay method of Russell (1962). The inoculated cells were seeded into 1i in.…”

Section: Hs Virusmentioning

confidence: 99%

“…The production of cell-free virus from primary human thyroid cells (Caunt, 1963) provides a more convenient laboratory source of cellfree virus and this paper describes the method we have used for detecting V-Z virus neutralizing antibody in the sera of chickenpox and zoster patients and in pools of human gamma-globulin. Kapsenberg (1964) and Ross, Subak Sharpe & Ferry (1965) showed some crossreactions between Herpes simplex (HS) and V-Z virus in complement-fixation (CF) tests so we also tested most of our sera with HS virus to see whether any crossneutralization could be demonstrated. The paired sera were also tested for CF antibody to both V-Z and HS antigens.…”

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confidence: 99%

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Neutralization tests with varicella-zoster virus

Caunt

Shaw

1969

J. Hyg.

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Varicella-zoster (V-Z) virus was first cultivated in tissue culture by Weller & Stoddard (1952). Subsequently the fluorescent antibody technique was used by Weller & Coons (1954) to demonstrate that antibodies to the virus are present in convalescent chickenpox and zoster sera, but few neutralization tests have been done with the agent because of the difficulty in obtaining virus in a cell-free state from most tissue culture systems. Weller & Witton (1958) showed that the ability of intact infected human embryonic fibroblasts to transmit virus to fresh tissue culture monolayers could be partially neutralized by antiserum if the antiserum was incorporated in the medium of the inoculated tissue cultures. The cytopathic effects of the virus were not suppressed completely but the number of foci of infection was greatly reduced and the foci themselves were altered in appearance. Taylor-Robinson (1959) described neutralization tests in which vesicle fluid taken directly from patients was used as a source of cell-free virus. This system gave satisfactory results but its application was limited by the difficulty of obtaining sufficient vesicle fluid. The production of cell-free virus from primary human thyroid cells (Caunt, 1963) provides a more convenient laboratory source of cellfree virus and this paper describes the method we have used for detecting V-Z virus neutralizing antibody in the sera of chickenpox and zoster patients and in pools of human gamma-globulin. Kapsenberg (1964) and Ross, Subak Sharpe & Ferry (1965) showed some crossreactions between Herpes simplex (HS) and V-Z virus in complement-fixation (CF) tests so we also tested most of our sera with HS virus to see whether any crossneutralization could be demonstrated. The paired sera were also tested for CF antibody to both V-Z and HS antigens.

show abstract

Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

“…Neutralization tests were carried out by the method described by Ross et al (1965) using the plaque assay method of Russell (1962). The inoculated cells were seeded into 1i in.…”

Section: Hs Virusmentioning

confidence: 99%

mentioning

confidence: 99%

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Neutralization tests with varicella-zoster virus

Caunt

Shaw

1969

J. Hyg.

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“…It was at this point that the genetic relationship between VZV and HSV-1 became of paramount importance. The literature documenting a weak serological relationship between the two viruses developed many years ago (Kapsenberg, 1964;Ross et al, 1965). The first evidence of a genetic relationship arose from the experiments of Davison & Wilkie (1983), in which radiolabelled VZV DNA was hybridized to Southern blots of HSV-1 DNA fragments.…”

Section: A J Davisonmentioning

confidence: 99%