Antigenic, microbicidal and antiparasitic properties of an l-amino acid oxidase isolated from Bothrops jararaca snake venom

Ciscotto, P.; Ávila, Ricardo Andrez Machado de; Coelho, Eduardo Antônio Ferraz; Oliveira, Jamil S.; Diniz, Cláudio Galuppo; Farias, Luiz M.; Carvalho, Maria Auxiliadora Roque de; Maria, W.S.; Sanchez, Eládio Flores; Borges, Arnaldo Chaer; Chávez-Olórtegui, Carlos

doi:10.1016/j.toxicon.2008.12.004

Cited by 115 publications

(88 citation statements)

References 49 publications

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“…Similar results were previously described in bioassays with venoms (Bustillo et al, 2008;Afc et al, 2010). One explanation for this situation is the existence of an optimal concentration for inhibition or that interactions between the venom compounds at different concentrations cause interference with growth inhibition (Haeberli et al, 2000;KuhnNentwig et al, 2012;Ciscotto et al, 2009). Previous studies corroborate the last explanation in that the peptide fraction of the venom from Bothrops jararaca showed higher inhibitory activity on the growth of Candida albicans than the unfractionated venom (Gomes et al, 2005).…”

Section: Discussionsupporting

confidence: 72%

Evaluation of antifungal activity of snake venoms from the Amazon forest

Neves¹,

Sousa²,

Carriccedil³

et al. 2015

J. Yeast Fungal Res.

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In recent years, many antimicrobial peptides have been found in the venoms of animals from different sources and have been intensively studied to elucidate their ability to inhibit the growth of potential pathogenic microorganisms. The aim of this study was to characterize and evaluate the in vitro antifungal activity of crude venom from two amazonian snakes: Bothrops atrox and Crotalus durissus ruruima. The molecular profile of representative proteins from the venom samples was obtained by reversed-phase high-performance liquid chromatography (RP-HPLC) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Fungal inhibition was investigated by microdilution assays against two Candida albicans strains. Based on the chromatography and electrophoresis analyses, the venom from B. atrox and C. durissus ruruima were characterized. In addition, the venoms (400 µg/mL) were not able to cause significant inhibition (> 50%) of the growth of C. albicans KL-07, at only 9.09% (200 µg/mL) and 7.88% (400 µg/mL), respectively, and neither presented any influence on the growth of strain C. albicans ATCC 36232.

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Section: Discussionsupporting

confidence: 72%

Evaluation of antifungal activity of snake venoms from the Amazon forest

Neves¹,

Sousa²,

Carriccedil³

et al. 2015

J. Yeast Fungal Res.

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“…Therefore, most of the biological effects of LAAO may be due to the secondary effect of hydrogen peroxide generated during the specific catalytic activity (Ponnudurai et al, 1994). This hypothesis can be supported when catalase is added to the medium in experimental conditions, when the toxic activity is totally abolished (Toyama et al, 2006;Izidoro et al, 2006;Rodrigues et al, 2009;Ciscotto et al, 2009).…”

Section: Discussionmentioning

confidence: 97%

Bothrops pirajai snake venom L-amino acid oxidase: in vitro effects on infection of Toxoplasma gondii in human foreskin fibroblasts

Izidoro¹,

Alves²,

Rodrigues³

et al. 2011

Rev. bras. farmacogn.

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Abstract:The effect of an L-amino acid oxidase isolated from Bothrops pirajai snake venom (BpirLAAO-I) was investigated on infection of Toxoplasma gondii in human foreskin fibroblasts (HFF). The cytotoxic activity of BpirLAAO-I on HFF cells showed a dose-dependent toxicity with median cytotoxic dose (TD50) of 11.8 µg/mL. BpirLAAO-I induced considerable dose-dependent decrease in the T. gondii infection index under two different conditions, treatment of tachyzoites before infection or treatment of HFF cells after infection. A maximal inhibition of infection (56%) was found for treatment before infection, with a median inhibitory dose (ID50) at 1.83 µg/mL and selectivity index (SI) at 6.45. For treatment after infection, it was observed a maximal inhibition of infection at 65%, ID50 of 1.20 µg/mL and SI of 9.83. The treatment before infection was also effective to reduce intracellular parasitism up to 62%, although presenting higher values of ID50 (3.14 µg/mL) and lower values of SI (3.76). However, treatment after infection was not effective, suggesting that the enzyme seems to have no effect on the parasite intracellular replication for this condition. In conclusion, BpirLAAO-I was more effective to inhibit the infection of neighboring cells and consequently parasite dissemination than primary infection and parasite replication. Thus, the effect of BpirLAAO-I described herein could be taken into account for the development of new synthetic anti-parasite therapeutic agents.

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“…venoms have been shown to have antiparasitic activity, against Leishmania spp. (Tempone et al, 2001) and Trypanosoma cruzi (Deolindo et al, 2005); antimicrobial effects on Pseudomonas aeruginosa, Candida albicans, Escherichia coli, and Staphylococcus aureus (Ciscotto et al, 2009;Rodrigues et al, 2004;Torres et al, 2010); and inhibitory effect on in vitro invasion and replication of Toxoplasma gondii (Bastos et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

In vitroandin vivogenotoxic evaluation ofBothrops moojenisnake venom

Zobiole

Caon

Bertól

et al. 2014

Pharmaceutical Biology

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Context: Bothrops moojeni Hoge (Viperidae) venom is a complex mixture of compounds with therapeutic potential that has been included in the research and development of new drugs. Along with the biological activity, the pharmaceutical applicability of this venom depends on its toxicological profile. Objective: This study evaluates the cytotoxicity and genotoxicity of the Bothrops moojeni venom (BMV). Material and methods: The in vitro cytotoxicity and genotoxicity of a pooled sample of BMV was assessed by the MTT and Comet assay, respectively. Genotoxicity was also evaluated in vivo through the micronucleus assay. Results: BMV displayed a 50% cytotoxic concentration (CC 50 ) on Vero cells of 4.09 mg/mL. Vero cells treated with 4 mg/mL for 90 min and 6 h presented significant (p50.05, ANOVA/NewmanKeuls test) higher DNA damage than the negative control in the Comet assay. The lower DNA damage found after 6 h compared with the 90 min treatment suggests a DNA repair effect. Mice intraperitoneally treated with BMV at 10, 30, or 80 mg/animal presented significant genotoxicity (p50.05, ANOVA/Newman-Keuls test) in relation to the negative control after 24 h of treatment. Contrary to the in vitro results, no DNA repair seemed to occur in vivo up to 96 h post-venom inoculation at a dose of 30 mg/animal. Discussion and conclusion: The results show that BMV presents cyto-and genotoxicity depending on the concentration/dose used. These findings emphasize the importance of toxicological studies, including assessment of genotoxicity, in the biological activity research of BMV and/or in the development of BMV-derived products.

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Antigenic, microbicidal and antiparasitic properties of an l-amino acid oxidase isolated from Bothrops jararaca snake venom

Cited by 115 publications

References 49 publications

Evaluation of antifungal activity of snake venoms from the Amazon forest

Evaluation of antifungal activity of snake venoms from the Amazon forest

Bothrops pirajai snake venom L-amino acid oxidase: in vitro effects on infection of Toxoplasma gondii in human foreskin fibroblasts

In vitroandin vivogenotoxic evaluation ofBothrops moojenisnake venom

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