Antigenic groupings of 90 rhinovirus serotypes

Abstract

We have completed production in rabbits of potent antisera to the 90 classified rhinovirus serotypes by using methods previously described (M. K. Cooney and G. E. Kenny, Proc. Soc. Exp. Biol. Med. 133:645-650, 1970). Systematic testing by neutralization tests has revealed significant numbers of cross-relationships among rhinovirus types, some of which have already been reported. Herein, our observations are compared with cross-reactions reported in National Institutes of Health reference guinea pig antisera. Also, original rhinovirus isolates, representing serotypes known to be antigenically related to other rhinoviruses, were tested against rabbit antisera to the related serotypes. These tests revealed extensive antigenic variation among isolates identified as rhinovirus 12:78 or 36:58, which are reciprocally related pairs, 41, reciprocally related to 13, and 67, which is related to both 9 and 32. If the rhinovirus serotypes were grouped according to antigenic relationships, 50 types could be included in 16 groups.

A novel group of rhinoviruses is associated with asthma hospitalizations

“…Several of these serotype pairs have been shown previously to cross-react, and 2 (HRV8 and HRV95) were likely the same strain. 28,46,47 HRVBs had a mean nucleotide identity of 77.3% (minimum of 58.8% and maximum of 88.8%).…”

Pan‐Viral Screening of Respiratory Tract Infections in Adults With and Without Asthma Reveals Unexpected Human Coronavirus and Human Rhinovirus Diversity

“…None of the divergent HRV'X' isolates were culturable; thus, unambiguous detection and classification of these isolates by conventional serotyping [34], drug susceptibility [35], or receptor-type usage assays [36] was not possible. Recovery of complete coding sequence from 2 of the HRV'X' isolates (HRV'X'-1 and HRV'X'-2) and analysis of their sequence identity with a representative subset of 27 fully sequenced HRVA subgroup genomes and 7 fully sequenced HRVB subgroup genomes [37] indicated that these HRV'X' isolates were indeed HRVs.…”

Reliable quantification of rhinovirus species C using real-time PCR

“…Rhinoviruses (RV) are a common cause of acute respiratory infection in people of all ages (Fawkner-Corbett et al, 2015;Puro et al, 2005). RVs are antigenically diverse, thus people can be infected with different RV types over the course of a lifetime (Cooney et al, 1982(Cooney et al, , 1975. The spectrum of disease associated with this group of viruses can range from asymptomatic infection, mild upper respiratory tract infection (common cold) to severe lower respiratory tract infections which may include bronchiolitis or pneumonia (Choi et al, 2015;Iwane et al, 2011;Luchsinger et al, 2014).…”