Antigenic expression in hepatocellular carcinoma

Thung, S N; Gerber, Michael A.; Popper, H

doi:10.1016/0016-5085(78)90663-7

Cited by 4 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of AFP is not specific for HCC as it may be seen in metastatic tumours such as from the ovary or pancreas, and is of low sensitivity, being observed in no more than 50% of HCCs when immunostaining is performed. 5 Hurlimann and Gardiol 2 obtained staining for AFP in 61.5% of cases and attributed the marked variation of positivity to the weak staining in many instances. Non-tumoral hepatocytes may also stain for AFP.…”

Section: Discussionmentioning

confidence: 97%

“…The demonstration of bile in the tumour is specific for HCC but of low sensitivity, histochemical staining being positive in only 5-33% of tumours. 5,[16][17][18] Mucin secretion is common in CCs and metastatic adenocarcinomas but is very uncommon in HCCs. Eosinophilic cytoplasmic globules that are periodic acid-Schiff positive and diastase-resistant may be observed in 10-15% of HCCs and represent alpha-fetoprotein (AFP), ␣-1-antitrypsin or ␣-1-antichymotrypsin.…”

Section: Discussionmentioning

confidence: 99%

“…Various immunohistochemical markers have been advocated for the identification of these tumours and include ␣-fetoprotein (AFP), ␣-1-antitrypsin, carcinoembryonic antigen (CEA), [1][2][3][4][5][6] factor XIIIa, 7 ferritin, 6 albumin 8 and cytokeratins 8, 18, 7 and 19. 2,9,10 However, the use of these markers has met with varying success and none has been proven to be specific.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hep Par 1 and selected antibodies in the immunohistological distinction of hepatocellular carcinoma from cholangiocarcinoma, combined tumours and metastatic carcinoma

et al. 1998

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hep Par 1 and selected antibodies in the immunohistological distinction of hepatocellular carcinoma from cholangiocarcinoma, combined tumours and metastatic carcinoma

et al. 1998

View full text Add to dashboard Cite

show abstract

“…Sections were carefully analyzed for the presence of ground glass hepatocytes. Immunohistochemical studies for HBsAg and hepatitis B core antigen (HBcAg) were performed on deparaffinized histologic tissue sections using polyclonal antibody to HBsAg and HBcAg (Dako, Carpinteria, CA) as previously described 20. Cytoplasmic staining for HBsAg and nuclear and cytoplasmic staining for HBcAg were interpreted as positive findings.…”

Section: Methodsmentioning

confidence: 99%

Histopathology and clinical correlates of end-stage hepatitis B cirrhosis: A possible mechanism to explain the response to antiviral therapy

Sigal

Ala²,

Ivanov³

et al. 2004

Liver Transpl

View full text Add to dashboard Cite

In chronic liver disease associated with histological necroinflammation, clinical severity is frequently greater in those with higher grades of activity. Conventional wisdom assumes that necroinflammation is mild or absent in patients with end-stage hepatitis B virus (HBV) cirrhosis due to the frequent presence of mildly elevated aminotransferase levels, the absence of hepatitis B e antigen (HBeAg), and low or undetectable HBV deoxyribonucleic acid (DNA) levels. However, a histopathologic analysis of such patients has not been undertaken. The aims of this study were 1) to assess severity and histological features of inflammation, 2) to correlate the severity of inflammation with biochemical and virologic parameters, and 3) to define the relationship between inflammation and clinical severity in explanted livers from patients undergoing liver transplantation for HBV cirrhosis. Characteristics of 34 consecutive patients undergoing liver transplantation for HBV cirrhosis were correlated with inflammation and immunohistological findings in the explanted livers. High-grade inflammation (grades 3 and 4) was found in many cases (47.1% interface hepatitis; 14.8% lobular inflammation; and 20.6% portal inflammation). The presence of positive cytoplasmic staining for hepatitis B core antigen (HBcAg) was associated with grade 3 or 4 interface hepatitis (P ‫؍‬ .046) and lobular hepatitis (P ‫؍‬ .005). There was no correlation between inflammatory activity and age, Asian ethnicity, aminotransferase levels, total bilirubin levels, HBeAg seropositivity, and detect-

show abstract

“…Liver tissues were frozen in a mixture of dry ice and isopentane, and stored at -70°C until use. Polyclonal antisera to HBsAg (goat anti-HBs) and HBcAg (rabbit anti-HBc) were obtained from Dako Corporation (Santa Barbara, Calif.) and characterized as described previously (13). Goat anti-HBs reacted with P24 and GP27 as well as the large and middle HBsAg polypeptides as demonstrated by immunoblot (Gilja, B. K. et al, unpublished data).…”

Section: Methodsmentioning

confidence: 99%

Demonstration of pre-S polypeptides of hepatitis B virus in infected livers

et al. 1986

View full text Add to dashboard Cite

The large (pre-S1), middle (pre-S2) and major (P24) polypeptides of HBsAg have been defined in detail, but their role in hepatitis B virus infection is not known. Therefore, we studied the expression of pre-S1, pre-S2 and P24 in the liver of 15 patients with acute or chronic hepatitis B virus infection using monoclonal and polyclonal antibodies in a double staining immunofluorescence method. The pre-S and major HBsAg polypeptides were co-expressed in the hepatocyte cytoplasm of all patients except for one case of chronic active hepatitis and cirrhosis. HBcAg was present in hepatocyte nuclei of nine patients suggestive of active hepatitis B virus replication. These studies support the hypothesis that the pre-S polypeptides represent essential components of the envelope of hepatitis B virus.

show abstract

Antigenic expression in hepatocellular carcinoma

Cited by 4 publications

References 0 publications

Hep Par 1 and selected antibodies in the immunohistological distinction of hepatocellular carcinoma from cholangiocarcinoma, combined tumours and metastatic carcinoma

Hep Par 1 and selected antibodies in the immunohistological distinction of hepatocellular carcinoma from cholangiocarcinoma, combined tumours and metastatic carcinoma

Histopathology and clinical correlates of end-stage hepatitis B cirrhosis: A possible mechanism to explain the response to antiviral therapy

Demonstration of pre-S polypeptides of hepatitis B virus in infected livers

Contact Info

Product

Resources

About