Antigenic Diversity Among Portuguese Clinical Isolates of <i>Helicobacter pylori</i>

Vitoriano, Inês; Rocha-Gonçalves, Alexandra; Carvalho, Teresa Bento de; Oleastro, Mónica; Calado, Cecı́lia R.C.; Roxo-Rosa, Mónica

doi:10.1111/j.1523-5378.2011.00825.x

Cited by 10 publications

(32 citation statements)

References 47 publications

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“…This multimeric protein of approximately 1100 kDa is composed of twelve small subunits, UreA (27 kDa), and twelve large subunits, UreB (62 kDa) [34]. In agreement with our previous findings [35], urease (UreA plus UreB) was one of the most abundant proteins in the proteome of all of the H. pylori strains in the current study. However, the strains collected from children with DU had higher levels of both UreA and UreB, than either the NUD or the GU strains.…”

Section: Discussionsupporting

confidence: 93%

“…Bacterial total protein extracts and their separation by 2DE was performed as previously described [35]. Briefly, 800–1000 µg of protein in 450 µL re-hydration buffer were loaded onto 18 cm Immobiline DryStrips (GE Healthcare, Uppsala, Sweden) with a non-linear wide range pH gradient (pH 3–11).…”

Section: Methodsmentioning

confidence: 99%

“…Scanning was carried out at 300 dpi and 16 bit grayscale. Images were analyzed using the ImageMaster™ 2D Platinum software (GE Healthcare) as before [35], taking into account the standardized relative intensity volume of spots (or % Vol, i.e. the volume of each spot over the volume of all spots in the gel).…”

Section: Methodsmentioning

confidence: 99%

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Ulcerogenic Helicobacter pylori Strains Isolated from Children: A Contribution to Get Insight into the Virulence of the Bacteria

et al. 2011

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Infection with Helicobacter pylori is the major cause for the development of peptic ulcer disease (PUD). In children, with no other etiology for the disease, this rare event occurs shortly after infection. In these young patients, habits of smoking, diet, consumption of alcohol and non-steroid anti-inflammatory drugs and stress, in addition to the genetic susceptibility of the patient, represent a minor influence. Accordingly, the virulence of the implicated H. pylori strain should play a crucial role in the development of PUD. Corroborating this, our in vitro infection assays comparing a pool of five H. pylori strains isolated from children with PUD to a pool of five other pediatric clinical isolates associated with non-ulcer dyspepsia (NUD) showed the greater ability of PUD strains to induce a marked decrease in the viability of gastric cells and to cause severe damage in the cells cytoskeleton as well as an impairment in the production/secretion of mucins. To uncover virulence features, we compared the proteome of these two groups of H. pylori strains. Two-dimensional gel electrophoresis followed by mass-spectrometry allowed us to detect 27 differentially expressed proteins between them. In addition to the presence of genes encoding well established virulence factors, namely cagA, vacAs1, oipA “on” status, homB and jhp562 genes, the pediatric ulcerogenic strains shared a proteome profile characterized by changes in the abundance of: motility-associated proteins, accounting for higher motility; antioxidant proteins, which may confer increased resistance to inflammation; and enzymes involved in key steps in the metabolism of glucose, amino acids and urea, which may be advantageous to face fluctuations of nutrients. In conclusion, the enhanced virulence of the pediatric ulcerogenic H. pylori strains may result from a synergy between their natural ability to better adapt to the hostile human stomach and the expression of the established virulence factors.

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Section: Discussionsupporting

confidence: 93%

Section: Methodsmentioning

confidence: 99%

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Ulcerogenic Helicobacter pylori Strains Isolated from Children: A Contribution to Get Insight into the Virulence of the Bacteria

et al. 2011

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“…All identified H. pylori strains possess the VacA gene, however, most of H. pylori associated with serious gastric diseases present a VacA-s1 genotype (also designated as VacA+), in relation to others genotypes associated to non functional VacA. The H. pylori strains designated as type I strains are characterized by Cag+ and Vac-s1 genotypes, and are associated with increased gastric mucosal inflammatory cell infiltration and increased gastric epithelial injury, compared with strains that do not express these factors, being therefore most involved in gastroduodenal disease induction [1,3,6]. Furthermore these latter strains seem to be more difficult to eradicate with antibiotic treatment [7].…”

Section: Introductionmentioning

confidence: 98%

Molecular fingerprint of human gastric cell line infected by Helicobacter pylori

Sampaio

Cunha

Rosa

et al. 2015

2015 IEEE 4th Portuguese Meeting on Bioengineering (ENBENG)

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Helicobacter pylori infection represents a serious health problem, given its association with serious gastric diseases as gastric ulcers, cancer and MALT lymphoma. Currently no vaccine exists and antibiotic-based eradication therapy is already failing in more than 20% of cases. To increase the knowledge on the infection process diverse gastric cell lines, e.g. the adenocarcinona gastric (AGS) cell line, are routinely used has in vitro models of gastric epithelia. In the present work the molecular fingerprint of infected and non-infected AGS cell lines, by diverse H. pylori strains, was acquired using vibrational infrared spectroscopy. These molecular fingerprints enabled to discriminate infected from non-infected AGS cells, and infection due to different strains, by performing Principal Component Analysis. It was also possible to estimate, from the AGS cells molecular fingerprint, the effect of the infection on diverse biochemical and metabolic cellular status. In resume infra-red spectroscopy enabled the acquisition of infected AGS cells molecular fingerprint with minimal sample preparation, in a rapid, high-throughput, economic process yielding highly sensitive and informative data, most useful for promoting critical knowledge on the H. pylori infection process.

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“…Its urease enzyme, a Ni 2+ -containing dodecameric protein of approximately 1100 kDa, composed of 12 small subunits, UreA (27 kDa), and 12 large subunits, UreB (62 kDa), catalyzes the hydrolysis of urea into ammonia and carbon dioxide, buffering both the bacteria cytoplasm and periplasm [39]. Accounting for 5-10% of the total protein content, urease is one of the most abundant proteins in the H. pylori proteome [16,30]. Probably due to the toxicity of ammonia, urease activity is known to be dependent on low pH and/or Ni 2+ concentration conditions [39,40], being essential for bacteria survival only under acidic conditions.…”

Section: Acid Resistance and Motilitymentioning

confidence: 99%

Helicobacter pylori—Associated Dyspepsia in Paediatrics

Roxo-Rosa¹,

Oleastro²,

Lopes³

2013

Dyspepsia - Advances in Understanding and Management

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Antigenic Diversity Among Portuguese Clinical Isolates of Helicobacter pylori

Abstract: This study showed evidence of the variability of antigenic pattern among H. pylori strains. We believe that this fact contributes to the failure of anti-H. pylori vaccines and the low accuracy of serological tests based on a low number of proteins or antigens of only one strain.

Cited by 10 publications

References 47 publications

Ulcerogenic Helicobacter pylori Strains Isolated from Children: A Contribution to Get Insight into the Virulence of the Bacteria

Ulcerogenic Helicobacter pylori Strains Isolated from Children: A Contribution to Get Insight into the Virulence of the Bacteria

Molecular fingerprint of human gastric cell line infected by Helicobacter pylori

Helicobacter pylori—Associated Dyspepsia in Paediatrics

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