Antigenic Dissimilarity of Streptokinases

Weinstein, Louis

doi:10.3181/00379727-83-20460

Cited by 16 publications

(8 citation statements)

References 2 publications

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“…The streptokinase gene from group C Streptococcus equisimiiis {skc) has been cloned and sequenced (MaIke and Ferretti, 1984;MaIke ef al., 1985) and DNA hybridization studies of skc with DNA from a variety of different groups and strains of streptococci have suggested the existence of a heterogeneity among streptokinases (Huang et ai., 1984;. These observations confirm previous immunologicat and chemical studies, which have provided evidence for a heterogeneity of streptokinases produced by various streptococci (Dillon and Wannamaker, 1965;Gerlach and Kohler, 1977;1979;Houbaand Hana, 1966;Weinstein, 1953). To evaluate some of these differences in more detail, we have cloned and sequenced a streptokinase gene from a group A S. pyogenes M type 49 strain, termed ska.…”

Section: Introductionsupporting

confidence: 79%

The streptokinase gene of group A streptococci: cloning, expression in Escherichia coli, and sequence analysis

Huang

Malke

Ferretti

1989

Molecular Microbiology

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The gene specifying the group A streptokinase (ska) gene was cloned from an M type 49 strain of Streptococcus pyogenes and shown to express in Escherichia coli. The nucleotide sequence of the DNA fragment carrying ska was determined and compared to that of the group C streptokinase gene (skc). There is 90% sequence identity between the two genes, with highly conserved transcription and translation control regions. The deduced amino acid sequence of the group A streptokinase (SKA) contains the same number of amino acids as that of group C streptokinase, with 85% sequence identity between the two proteins. Among 440 amino acid residues specified by the coding sequence, there are 62 non-identical residues with 45 conserved and 17 non-conserved residues. The non-identical residues are located in two major regions, spanning residues 174 to 244 and 270 to 290, with 40 and 10 amino acid changes, respectively. The sequence differences provide an explanation at the molecular level for the previous findings of immunological and chemical heterogeneity among streptokinase produced by pathogenic streptococci.

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Section: Introductionsupporting

confidence: 79%

The streptokinase gene of group A streptococci: cloning, expression in Escherichia coli, and sequence analysis

Huang

Malke

Ferretti

1989

Molecular Microbiology

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“…Numerous investigators, most notably Weinstein (34), and Gerlach and Kohler (30,35) have shown that streptokinases isolated from groups A, C, and G streptococci are antigenically different from each other. Dillon and Wannamaker (36) found two physically and immunologically different types of streptokinases produced by different group A streptococci, and these were different from streptokinase from group C streptococci.…”

Section: Discussionmentioning

confidence: 99%

Purification and partial characterization of the nephritis strain-associated protein from Streptococcus pyogenes, group A.

Johnston¹,

Zabriskie²

1986

The Journal of Experimental Medicine

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We report the isolation and purification of the nephritis strain-associated protein (NSAP) first described by Villareal et al. (8). Amino acid analysis, and determination of the first 21 amino-terminal amino acids indicated that this 46 kD protein is a streptokinase. Biochemical analysis confirmed that NSAP could act as a plasminogen activator; immunological investigations indicated that NSAP is antigenically different from streptokinase from group C streptococcus, and possibly represents a unique streptokinase. It is this uniqueness that may contribute to the role of NSAP in the pathogenesis of acute poststreptococcal glomerulonephritis.

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“…Previous reports concerning the similarity or dissimilarity among streptokinases produced by different serologic groups of beta hemolytic streptococci, or by various strains of Group A streptococci, have been based on immunologic studies employing human sera. The most recent and best quantitated investigations are those of Kaplan (25) and Weinstein (26).…”

Section: Discussionmentioning

confidence: 99%

Physical and Immunological Differences Among Streptokinases

Dillon

Wannamaker

1965

The Journal of Experimental Medicine

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Two strains of Group A streptococci have been shown to produce streptokinases with different physical and immunologic characteristics. These streptokinases have been designated SK-A and SK-B. Examination of additional strains of Group A streptococci has indicated that SK-A is the more commonly encountered streptokinase. Studies comparing the two Group A streptokinases with streptokinase of Group C origin have indicated that the latter streptokinase is intermediate between SK-A and SK-B in regard to its electrophoretic mobility. Immunological data suggest that the Group C streptokinase differs from SK-B but is closely related to SK-A.

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Antigenic Dissimilarity of Streptokinases

Cited by 16 publications

References 2 publications

The streptokinase gene of group A streptococci: cloning, expression in Escherichia coli, and sequence analysis

The streptokinase gene of group A streptococci: cloning, expression in Escherichia coli, and sequence analysis

Purification and partial characterization of the nephritis strain-associated protein from Streptococcus pyogenes, group A.

Physical and Immunological Differences Among Streptokinases

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