Antigenic differences in the H proteins of canine distemper viruses

Iwatsuki, K.; Tokiyoshi, Sachio; Hirayama, Norio; Nakamura, Kenzo; Ohashi, Kousaburo; Wakasa, Chiaki; Mikami, Takeshi; Kai, Chieko

doi:10.1016/s0378-1135(99)00172-8

Cited by 71 publications

(55 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The H protein is responsible for viral attachment and cell tropism and is one of the most variable Morbillivirus proteins that has been frequently used to evaluate genetic diversity between CDV isolates (Haas et al, 1997;Mochizuki et al, 1999;Greene and Vandevelde, 2012). Additionally, nucleotide (nt) and amino acid (aa) variation in the H gene might disrupt important epitopes by affecting neutralization-related sites (Blixenkrone-Möller et al, 1992;Haas et al, 1997;Iwatsuki et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic analyses of the hemagglutinin gene of wild-type strains of canine distemper virus in southern Brazil

Negrão

Gardinali²,

Headley³

et al. 2013

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. This study examined the phylogenetic relationship of strains of canine distemper virus (CDV) collected from Paraná State, Brazil, based on the hemagglutinin gene. Urine samples were collected from 4 dogs from northern Paraná State that demonstrated clinical manifestations of canine distemper. The participation of CDV was initially confirmed by RT-PCR targeting the nucleocapsid protein, after which the complete hemagglutinin gene was sequenced from each sample. Sequences were deposited in and compared with those already in GenBank. Phylogenetic analyses, using amino acid and nucleotide sequences based on the hemagglutinin gene, demonstrated that these strains of CDV are closely related to those from the Europe 1 lineage of CDV, with marked differences from other recognized geographical clusters of CDV isolates and from the vaccine strains. The strains of CDV from this region of southern Brazil appear to be related to those from Europe 1.

show abstract

Section: Introductionmentioning

confidence: 99%

Phylogenetic analyses of the hemagglutinin gene of wild-type strains of canine distemper virus in southern Brazil

Negrão

Gardinali²,

Headley³

et al. 2013

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…Nine out of eleven (81.8%) and three out of nine (33.3%) lions were positive for CDV antibody in zoo A and zoo B, respectively. We also examined the existence of neutralizing antibody against CDV in the same samples as described previously [15]. Neutralizing antibody titers were well correlated with the results of ELISA (Table 1).…”

mentioning

confidence: 80%

Prevalence of Canine Distemper Virus, Feline Immunodeficiency Virus and Feline Leukemia Virus in Captive African Lions (Panthera leo) in Japan

Endo

Uema

Miura

et al. 2004

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

ABSTRACT. Sero-prevalences of canine distemper virus (CDV), feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) were evaluated in 20 captive lions in two Japanese zoos. Anti-CDV antibody was detected in 13 of 20 lions. We could pursue ant ibody responses against CDV in three lions back to 1996. Sera collected in 1996 were negative for anti-CDV antibody, therefore, all of them showed sero-conversion in 2000. This result suggested that the epidemic of CDV infection in this zoo might have happened between 1996 and 2000. The lions were also examined for FIV and FeLV infections. We had no evidence for FeLV infection but eight lions were sero-positive for anti-FIV antibody.

show abstract

“…Since the hydrophobicity of the amino acid sequence of a protein reflects its antigenic determinants (Jameson and Wolf, 1988), the variation of the epitope aa 157-169 may be related to the failure of the protection of the dog after the CDV Onderstepoort vaccination. Surveys conducted in 2 animal hospitals in Tokyo, Japan revealed that 2/3 of vaccinated dogs were diagnosed with CD, and most of them had high neutralization antibody titers against the Onderstepoort strain, but very low titers against wt strains, suggesting that the antigenic epitopes on the envelope of vaccine strain are different from wt strains (Ohashi et al, 1998;Iwatsuki et al, 2000). Moreover, Iwatsuki believed that different N-linked glycosylation sites could affect the immunogenicity of the H protein (Iwatsuki et al, 2000).…”

Section: Figmentioning

confidence: 99%

“…Surveys conducted in 2 animal hospitals in Tokyo, Japan revealed that 2/3 of vaccinated dogs were diagnosed with CD, and most of them had high neutralization antibody titers against the Onderstepoort strain, but very low titers against wt strains, suggesting that the antigenic epitopes on the envelope of vaccine strain are different from wt strains (Ohashi et al, 1998;Iwatsuki et al, 2000). Moreover, Iwatsuki believed that different N-linked glycosylation sites could affect the immunogenicity of the H protein (Iwatsuki et al, 2000). In our study, we found that the H protein of the XJ2 had 9 potential N-linked glycosylation sites, 4 of which were identical with those in the Onderstepoort vaccine strain, but 2 of which were unique to XJ2.…”

Section: Figmentioning

confidence: 99%

Characterization of a new dog isolate of canine distemper virus from China

Qiao¹,

Meng²,

C³

et al. 2012

View full text Add to dashboard Cite

Summary. -Canine distemper virus (CDV) is a highly contagious pathogen of dogs. Vaccination is an effective way to protect dogs from CDV infection, but occasionally fails. In the present study, a wild type (wt) CDV, named XJ2, was isolated from a dead vaccinated dog. The hemagglutinin (H) gene of the XJ2 was amplified and analyzed for the molecular characteristics including N-glycosylation sites, phylogenesis, hydrophobicity and epitopes. The data indicated that XJ2 was a genetic variant strain of CDV. CDV-sero-negative dogs were inoculated intranasally with XJ2, developed severe clinical symptoms and died, suggesting high virulence.

show abstract

Antigenic differences in the H proteins of canine distemper viruses

Cited by 71 publications

References 18 publications

Phylogenetic analyses of the hemagglutinin gene of wild-type strains of canine distemper virus in southern Brazil

Phylogenetic analyses of the hemagglutinin gene of wild-type strains of canine distemper virus in southern Brazil

Prevalence of Canine Distemper Virus, Feline Immunodeficiency Virus and Feline Leukemia Virus in Captive African Lions (Panthera leo) in Japan

Characterization of a new dog isolate of canine distemper virus from China

Contact Info

Product

Resources

About