Antigenic Cross-Reactivity of Staphylococcal Enterotoxins

Johnson, Howard M.; Bukovic, Joann A.; Kauffman, Peter E.

doi:10.1128/iai.5.5.645-647.1972

Cited by 17 publications

(12 citation statements)

References 4 publications

(5 reference statements)

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“…This group also isolated common antibody to A and E (12). Although reported (8,11), cross-reactivity of polyclonal antibody to SEA and SEB has not been confirmed (12). These investigations and the work reported here for monoclonal antienterotoxin suggest that there are two groups of toxins with common antigenic sites, B and Cl in one group and A, D, and E in the other.…”

Section: Discussionmentioning

confidence: 99%

Development of a monoclonal antibody capable of interacting with five serotypes of Staphylococcus aureus enterotoxin

Meyer¹,

Miller²,

Bennett³

et al. 1984

Appl Environ Microbiol

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A monoclonal antibody capable of binding to determinants shared in common by staphylococcal enterotoxin serotypes A, B, Cl, D, and E was developed. To accomplish this, BALB/c mice were immunized by alternating injections of serotypes A and D to enrich for spleen lymphocytes programmed to produce antibody to possible common determinants. These cells, fused with mutant myeloma cells (P3-X-63Ag8.653) produced hybrids that formed monoclonal antibodies to either serotype A only or to both serotypes A and D. A cloned hybridoma from the latter group produced an antibody (subclass immunoglobulin Gl) which interacted with five serotypes. Highest affinity was to B and C1. An immunomatrix consisting of this antibody cross-linked to protein A-Sepharose CL-4B with dimethyl pimelimidate was capable of binding enterotoxin. Bound toxin was eluted with diethylamine. Because of its ability to interact with all five serotypes, the monoclonal antibody should prove useful in the development of a rapid method for screening foods for the presence of staphylococcal enterotoxin.

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Section: Discussionmentioning

confidence: 99%

Development of a monoclonal antibody capable of interacting with five serotypes of Staphylococcus aureus enterotoxin

Meyer¹,

Miller²,

Bennett³

et al. 1984

Appl Environ Microbiol

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“…Four milligrams of crude SEA containing 0.72 mg of protein and 2.24 1Lg of SEA was dissolved in 0.5 ml of 0.125 M Tris buffer (pH 6.8) containing 10% glycerol and 0.001% bromphenol blue. This was mixed with 0.14 ml of a 0.4% solution of Trypsin (type III, 10,200 BAEE units per mg of protein; Sigma) in the same buffer as above and incubated for 2 or 4 1/2 h at 37°C. The volume was then increased to 1 ml with the same sample buffer containing 10% 3-mercaptoethanol and 2% sodium dodecyl sulfate (SDS).…”

Section: Methodsmentioning

confidence: 99%

“…Staphylococcal enterotoxins (SE) A (SEA), SEB, SEC,, SEC2, SEC3, SED, and SEE which cause food intoxications in humans are detected in foods on the basis of their specific reactivity in a double-gel immunodiffusion system (micro-Ouchterlony or microslide) with polyclonal antiserum against each of the SE. Though each of the SE bears a major distinct antigenic site (2), cross-reactivities among SE (1,3,10,11) demonstrated the existence of minor common antigenic site(s) among SE. Polyclonal antibodies directed against this minor site enabling detection of all SE have not been produced.…”

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confidence: 99%

Specificity and cross-reactivity of staphylococcal enterotoxin A monoclonal antibodies with enterotoxins B, C1, D, and E

Edwin¹,

Tatini²,

Maheswaran³

1986

Appl Environ Microbiol

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The cross-reactivity of monoclonal antibodies produced against staphylococcal enterotoxin A with purified and crude enterotoxins B, C,, D, and E and the specificity of such reactions were evaluated by the indirect enzyme-linked immunosorbent assay and immunoblotting of Western blots (from sodium dodecyl sulfatepolyacrylamide gel electrophoresis) followed by autoradiography. Purified and crude enterotoxins B were also tested with polyclonal antibodies. Specificity of reactivity was demonstrated by immunoblotting of crude enterotoxin A, crude enterotoxin A treated with trypsin, crude enterotoxin E, and also with crude A, B, Cl, and D that were pretreated with Sepharose-4B-linked normal rabbit immunoglobulin G to remove protein A. A band corresponding to each staphylococcal enterotoxin was seen with monoclonal antibodies under all conditions tested and also with crude and purified enterotoxin B with two different (rabbit and goat) polyclonal antisera.

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“…Staphylococcal enterotoxins C, (SEC1) and C2 (SEC2) were shown by double gel diffusion to share common major antigenic determinants, with each toxin having different minor ones (3). Enterotoxins B (SEB) and SEC1 were demonstrated by ammonium sulfate coprecipitation (7), radioimmunoassay (9), and neutralization studies (5) to contain similar antigenic determinants. Cross-reaction between enterotoxins A (SEA) and E (SEE) was observed in double gel diffusion plates and in neutralization studies (4).…”

mentioning

confidence: 99%

Isolation of specific and common antibodies to staphylococcal enterotoxins B, C1, and C2

Lee¹,

Robbins²,

Reiser³

et al. 1980

Infect Immun

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Staphylococcal enterotoxins C, and C2 elicit the production of antibodies in rabbits that give precipitin reactions with both toxins in gel diffusion plates. These enterotoxins also elicit the production of antibodies that are specific for each of the enterotoxins. Enterotoxin B elicits the production of antibodies in some rabbits that react with enterotoxins B, C,, and C2 in gel diffusion plates and in radioimmunoassay. Antibodies specific for enterotoxin B are produced also. Enterotoxin C, elicits the production of antibodies that cross-react weakly with

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Antigenic Cross-Reactivity of Staphylococcal Enterotoxins

Cited by 17 publications

References 4 publications

Development of a monoclonal antibody capable of interacting with five serotypes of Staphylococcus aureus enterotoxin

Development of a monoclonal antibody capable of interacting with five serotypes of Staphylococcus aureus enterotoxin

Specificity and cross-reactivity of staphylococcal enterotoxin A monoclonal antibodies with enterotoxins B, C1, D, and E

Isolation of specific and common antibodies to staphylococcal enterotoxins B, C1, and C2

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