Antigenic Cross-reactivity between Caprine Arthritis-Encephalitis, Visna and Progressive Pneumonia Viruses Involves All Virion-associated Proteins and Glycoproteins

Abstract

SUMMARYAntigenic relatedness between the virion-associated proteins of caprine arthritisencephalitis, visna and progressive pneumonia viruses was examined. Antigenic crossreactivity was assessed by immunoprecipitation of disrupted, radiolabeUed virus with goat, sheep and rabbit antisera, followed by resolution of the immunoprecipitation products by SDS-polyacrylamide gel electrophoresis. The results indicate that antigenic cross-reactivity between the caprine and ovine virus isolates involves all of the major virion-associated proteins and glycoproteins. The common antigenic determinants exhibited by virion structural proteins are immunogenic in goats, sheep and rabbits.

Variations in Lentiviral Gene Expression in Monocyte-derived Macrophages from Naturally Infected Sheep

“…In another sample, viral proteins were identified but the naturally infected macrophages failed to produce infectious particles. This type of infection differs from earlier findings showing that virus production in 'latently' infected monocytes could be induced by maturation-differentiation of the cells in vitro (Narayan et al, 1983 b;Gogolewski et al, 1985 ;Kennedy et al, 1985). We show here that this virusinduction process was predictable in cells from I5 infected sheep but not in cells from 10 others.…”

Routes of transmission and consequences of small ruminant lentiviruses (SRLVs) infection and eradication schemes

“…Radioimmunoprecipitation and radioimmunoassay are largely limited to experimental work and these methods are not considered as routine diagnostic procedures [51,77].…”

Imunofluorescência utilizando isolados brasileiros no diagnóstico sorológico de infecção por lentivírus em caprinos

“…Amostras de soro coletadas de animais em diferentes fases de infecção e a existência de isolados antigenicamente distintos também podem explicar os resultados discordantes nos dois testes (Cavirani et al 1998). A reação antigênica cruzada entre isolados de CAEV e MVV envolve todas as principais proteínas e glicoproteínas associadas ao virion (Gogolewski et al 1985). Em virtude dessa reatividade cruzada entre proteínas estruturais de lentivírus de pequenos ruminantes (SRLV), antígenos de MVV, que alcançam altos títulos in vitro, podem ser utilizados para detectar anticorpos específicos para SRLV tanto em ovinos como em caprinos.…”