Antigenic Conversion from HBeAg to HBeAg by Degradation of Hepatitis B Core Particles

Ohori, Hitoshi; Yamaki, Masahiro; Onodera, Satoshi; Yamada, Erika; Ishida, Nakao

doi:10.1159/000149110

Cited by 40 publications

(34 citation statements)

References 8 publications

(13 reference statements)

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“…wt. 21000 to 21500 is the only dominant polypeptide detectable in core particles that share the antigenicities of both HBcAg and HBeAg (Ohori et al, 1980a;Mackay et al, 1981 ;Yamaki et al, 1982). Furthermore, we found that a polypeptide with a tool.…”

Section: Introductionmentioning

confidence: 61%

“…In this phase, only HBeAg/1 is released from core particles and their morphology does not change at all. A second phase reaction is caused by acidic buffer (lower than pH 4.5), heat treatment at 100 °C for 10 s (Ohori et al, 1980a), sonication or 8 M-urea treatment. In another paper , we predicted the secondary structure of the polypeptide deduced from the nucleotide sequence.…”

Section: Discussionmentioning

confidence: 99%

“…21000. These smaller polypeptides could be derived from the major component protein of core particles (Ohori et al, 1980a;Yamaki et al, 1982). Miyakawa & Mayumi (1982) demonstrated that the core particle-derived polypeptide of mol.…”

Section: Discussionmentioning

confidence: 99%

“…An autopsy of liver (5 g) obtained from a patient who died from HBsAgpositive subacute hepatitis was the sole starting material for the experiment. Extraction and purification of core particles followed the same methods described in a previous paper (Ohori et al, 1980a).…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Immunological and Morphological Properties of HBeAg Subtypes (HBeAg/1 and HBeAg/2) in Hepatitis B Virus Core Particles

Ohori

Shimizu

Yamada

et al. 1984

Journal of General Virology

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Section: Introductionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Immunological and Morphological Properties of HBeAg Subtypes (HBeAg/1 and HBeAg/2) in Hepatitis B Virus Core Particles

Ohori

Shimizu

Yamada

et al. 1984

Journal of General Virology

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“…The close physical correlation between HBeAg and Dane particles was examined earlier (Ohori et al, , 1980Takahashi et al, 1979;Yoshizawa et al, 1979), and HBeAg is now Yamada et al, 1979Yamada et al, , 1981. In earlier reports from our laboratory (Yamada et al, 1979(Yamada et al, , 1981, it was demonstrated that free 'small' mol.…”

Section: Introductionmentioning

confidence: 65%

Comparison of the Antigenicity and Protein Composition of Hepatitis B Virus e Antigen Subtypes HBeAg/1 and HBeAg/2

Yamada

Ishida

Ohori

1983

Journal of General Virology

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SUMMARYHepatitis virus B e antigen subtypes HBeAg/1 and HBeAg/2 can be distinguished by a micro-Ouchterlony immunodiffusion assay and a solid-phase radioimmunoassay using serum IgG-associated HBeAgs and liver-and serum-derived IgG-free HBeAgs. The liver-and serum-derived HBeAg consisted of two different antigenicities, HBeAg/I and HBeAg/2. Both the liver-derived HBeAg/I and HBeAg/2 had mol. wt. of 30 000 (monomer) and 90 000 (trimer) and shared the same isoelectric point of 4-3 to 4.8. Approximately 80~ of liver-derived HBeAg (mol. wt. 30 000 and 90 000) had HBeAg/1 activity and the remaining 20~ was HBeAg/2. The serum-derived HBeAg/1 and HBeAg/2 were both associated with IgG to form heterogeneous moieties with apparent mol. wt. of 240000, 400000 and 540000 or were free, with mol. wt. 30000 (monomer) and 90 000 (trimer). The serum HBeAg of mol. wt. 90 000 was further differentiated into two molecular species, one trimer and the other associated with albumin. Large types of both HBeAg/1 and HBeAg/2 were recovered by isoelectrictric focusing at pH 5-5 to 7-5 (mol. wt. 240000), 5-7 to 8-0 (mol. wt. 400000) and 6.4 to 8-4 (mol. wt. 540000) respectively, while small types of both exhibited a narrower range of isoelectric points similar to liver-derived antigens. In most sera, antigenicity of half of the serum IgGassociated HBeAg was HBeAg/1 and the remaining 50~ was HBeAg/2, whereas 80~ of the free HBeAg had HBeAg/1 activity and the remaining 20 ~ was HBeAg/2. These results indicate that HBeAg/1 and HBeAg/2, although antigenically distinct, are very similar physicochemically with respect to both mol. wt. and isoelectric point.

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Virological significance of HBeAg subtypes (HBeAg/1 and HBeAg/2) in patients with type B hepatitis

et al. 1987

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In order to establish the virological significance of HBeAg subtypes (HBeAg/1 and HBeAg/2) during hepatitis B virus infection, HBsAg, HBeAg and hepatitis B virus DNA in serum and HBcAg in liver were determined quantitatively in relation to the detection of HBeAg subtypes in agar gel diffusion. Thirty-eight chronic HBsAg carriers with HBeAg, including 16 non-specific reactive hepatitis, 8 chronic persistent hepatitis, 11 chronic active hepatitis and 3 liver cirrhosis, who were seen at Tohoku University Hospital from 1983 to 1985, were examined. Significantly larger amounts of HBsAg, HBeAg and hepatitis B virus DNA in serum and HBcAg in liver were found in patients positive for both HBeAg/1 and HBeAg/2 in serum than in those positive for only HBeAg/1 or negative for both subtypes. These results suggest that the presence of HBeAg/2 in serum may reflect the occurrence of active viral replication. When the detection pattern of HBeAg subtypes was examined during serial follow-up for at least 1 year, three groups of patients were classified with respect to the presence of HBeAg/2, i.e., Type I, consistently positive for HBeAg/2; Type II, consistently negative for HBeAg/2, and Type III, intermittently positive for HBeAg/2. More than 80% of Type I patients were histologically diagnosed having as nonspecific reactive hepatitis, while more than 80% of Type II and III patients had more progressive liver diseases such as chronic persistent hepatitis, chronic active hepatitis and liver cirrhosis. These results suggest that the serial examination of HBeAg subtypes in serum may be important for more detailed evaluations of type B hepatitis.

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Antigenic Conversion from HBeAg to HBeAg by Degradation of Hepatitis B Core Particles

Cited by 40 publications

References 8 publications

Immunological and Morphological Properties of HBeAg Subtypes (HBeAg/1 and HBeAg/2) in Hepatitis B Virus Core Particles

Immunological and Morphological Properties of HBeAg Subtypes (HBeAg/1 and HBeAg/2) in Hepatitis B Virus Core Particles

Comparison of the Antigenicity and Protein Composition of Hepatitis B Virus e Antigen Subtypes HBeAg/1 and HBeAg/2

Virological significance of HBeAg subtypes (HBeAg/1 and HBeAg/2) in patients with type B hepatitis

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