Antigenic characterization of flavivirus structural proteins separated by isoelectric focusing

Abstract

Isoelectrofocusing of nonionic-detergent-disrupted flaviviruses separated the envelope glycoprotein of 53,000 to 58,000 daltons and the nucleocapsid protein of 14,000 daltons. The envelope protein and nucleocapsid protein were isolated at isoelectric points of pI 7.8 and 10.3, respectively. The antigenic determinants of St. Louis encephalitis, Japanese encephalitis, and dengue virus envelope and nucleocapsid proteins were examined by solid-phase competition radioimmunoassay. By the appropriate selection of antiserum and competing proteins, it was possible to distinguish type-specific, complex-reactive and flavivirus groupreactive antigenic determinants. The envelope glycoproteins of St. Louis encephalitis, Japanese encephalitis, and dengue viruses were found to contain each of these three classes of antigenic determinants. Most of the determinants on the envelope protein were type specific, some were complex reactive, and a small fraction were flavivirus group reactive. The nucleocapsid protein contained only flavivirus group-reactive antigenic determinants. Purified flaviviruses contain three classes of antigenic determinants: type specific, complex reactive, and flavivirus group reactive. Type-MATERIALS AND METHODS Viruses and cells. Virus strains used in these experiments were the Tampa Bay human-28 strain of SLE and the Nakayama, JaGAr-01, and Yokoshiba strains of JE viruses. Four prototype dengue 608

Studies on the Antigenic Structure of Japanese Encephalitis Virus Using Monoclonal Antibodies

“…This indicates that the JE species-specific HI site and the flavivirus genus-specific HI site are topologically distinct. Trent (21) has indicated that the envelope glycoprotein of flavivirus separated by isoelectric focusing after detergent treatment contained at least three antigenic determinants, species-, subgroup-, and genus-specific.…”

“…Comparative studies of the antigenic structure of Japanese encephalitis ( JE) virus have been performed by many investigators (1,5,12,17,21) since Hale and Lee (4) observed antigenic variation among the JE virus strains isolated in Malaya. However, the immunological classification of strains of JE virus is not yet clear.…”

Variation Among Strains of St. Louis Encephalitis Virus: Basis for a Genetic, Pathogenetic, and Epidemiologic Classification

“…-29, 31,33,34 1-7, 10, 15, 16, 18-20, 24, 25, 27. 32, 34, 36, 38 same as above 62% 2,7,12, 13,17, 18-24, 26,27 68V-1587 (TX, 66) (32/37) 86% 4,13,17,18,72) (27/37) 73% 2 4 6 , 13, 15-18,27 P-17787 (TX, 66) (22/37) 59% 2-4, 6, 12-15,21,22,27, 29, 30, 36, 37 1974-1975 1 , 4 , 6 , 8 , 12,22,31 J7352 (Jamaica, 62) (22133) 67% I , [3][4][5][6]9,10,[24][25][26]28 oligonucleotide fingerprints of isolates BFN-4585 and 68V1587 are quite similar to that of the "topotype." The fingerprints of viruses BFS-207 (CA), 72V-1165 ( N M ) , and P-17787 (TX) are quite distinct from the western "topotype" BFS-4876 from California.…”

An Enzyme‐Linked Immunosorbent Assay Using a Chaotropic Agent (Sodium Thiocyanate) for Serotype Specific Reaction between Crude Dengue Viral Antigen and Anti‐Dengue Mouse Antibody

“…On the other hand, there are several reports on the antigenic characteristics of the major envelope glycoprotein. Trent et al (22,23) suggested that the major envelope glycoprotein (E) of the flaviviruses contained at least three antigenic determinants: (1) flavivirus group reactive, (2) complex specific and (3) serotype specific. Henchal et al (8) observed four antigenic determinants: (1) flavivirus group-reactive, (2) dengue complex-specific, (3) dengue subcomplex-specific and (4) dengue serotype-specific using monoclonal antibodies against structural protein of dengue viruses.…”