Antigenic and genetic relatedness of eight Rickettsia tsutsugamushi antigens

Oaks, Edwin V.; Rice, Robert M.; Kelly, Daryl J.; Stover, C. Kendall

doi:10.1128/iai.57.10.3116-3122.1989

Cited by 55 publications

(43 citation statements)

References 25 publications

(32 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They express epitopes of the 150 kDa, 110 kDa, 72 kDa, 58 kDa, 56 kDa, 49 kDa, 47 kDa and 20 kDa polypeptide antigens [6]. The principal cell wall constituents of O. tsutsugamushi comprise the major strain variable 56-kDa protein as well as the antigenically variable 110-kDa, 47-kDa and 20-kDa proteins [6][7][8][9][10]. We developed a monoclonal antibody recognizing the 47 kDa antigen, which is a surface protein of O. tsutsugamushi.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Study of Scrub Typhus: A Report of Two Cases

Tseng

Yang

Liou

et al. 2008

The Kaohsiung J of Med Scie

View full text Add to dashboard Cite

Scrub typhus is a zoonotic disease caused by Orientia tsutsugamushi, which is transmitted by chiggers. The target cells of this rickettsia are poorly defined in humans. Immunohistochemical staining of tissue sections of patients with scrub typhus is helpful in investigating the target cells of this rickettsia in different organs. We studied two autopsy specimens by immunohistochemical staining using a specific antibody against O. tsutsugamushi. Rickettsiae were located in endothelial cells in all of the organs evaluated, namely heart, lung, brain, kidney, appendix and skin, within cardiac muscle cells and renal tubular epithelial cells, and in macrophages located in the lymph node, liver and spleen. In conclusion, O. tsutsugamushi may disseminate into multiple organs through endothelial cells and macrophages, resulting in the development of fatal complications.

show abstract

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Study of Scrub Typhus: A Report of Two Cases

Tseng

Yang

Liou

et al. 2008

The Kaohsiung J of Med Scie

View full text Add to dashboard Cite

show abstract

“…The ability of rickettsial pathogens, including A. marginale, to generate antigenic variants has been clearly established by the identification of antigenically distinct strains (7,(9)(10)(11)14). Variation among strains of Rickettsia tsutsugamushi, which causes an acute, nonpersistent infection, appears to be relatively stable (7,10). In contrast, within persistent rickettsia infections such as bovine anaplasmosis, stable antigenic variants may be accompanied by the more rapid variation necessary for persistence (9).…”

Section: Downloaded Frommentioning

confidence: 99%

Cyclic rickettsemia during persistent Anaplasma marginale infection of cattle

Kieser¹,

Eriks²,

Palmer³

1990

Infect Immun

134

View full text Add to dashboard Cite

Submicroscopic levels of Anaplasma marginale rickettsemia in persistently infected cattle were determined by using nucleic acid hybridization. Within individuals, the rickettsemia levels steadily increased from less than 104 infected erythrocytes per ml to a peak of more than 106 infected erythrocytes per ml and then rapidly declined. This logarithmic variation parallels the variation of the rickettsemia level seen in acute infection and suggests that cyclic emergence of antigenic variants is a mechanism of rickettsial persistence.

show abstract

“…However, methylation of DNA could have caused poor cutting with the restriction enzymes. Other possibilities are the presence of repeated sequences within the genes, as in the case of R. rickettsii (2) and Rickettsia tsutsugamushi (24), which detect similar sequences in other genes, or the presence of multiple copies of the genes or the flanking sequences, as has been suggested for R. tsutsugamushi (14). These can be verified by cloning and sequencing the complete genes.…”

Section: Discussionmentioning

confidence: 98%

Molecular cloning and analysis of recombinant major antigens of Ehrlichia risticii

1991

View full text Add to dashboard Cite

The genome of Ehrlichia risticii, the etiologic agent of Potomac horse fever, was cloned in the Xgtll expression vector. The efficiency of recombinant phage production with different restriction fragments of E. risticii DNA was generally between 20 and 95%. The antigen-positive frequency, detected by immunoscreening with E. risticii antibodies, was between 8 and 40 per 104 recombinants. Four (70, 55, 51, and 44 kDa) major antigens of E. risticii were identified from the recombinant phages by using recombinant antigen-selected monospecific antibodies. Characterization of three (70, 55, and 44 kDa) of these recombinant antigens indicated that the 70and 44-kDa polypeptides were ,I-galactosidase fusion products that were dependent on isopropylthiogalactoside induction for expression; they contained about 50 and 73%, respectively, of the native polypeptides. The 55-kDa antigen was a nonfusion protein expressed independently of isopropylthiogalactoside induction; it was a complete protein with a molecular weight identical to that of its native counterpart. The cloned E. risticii DNAs from of the recombinants expressing 70-, 55-, and 44-kDa proteins were 3.5, 3.9, and 4.8 kb, respectively, in size, and they were unique. The insert DNAs hybridized to multiple restriction fragments of the genomic DNA, the sum of the sizes of which was much greater than that of the corresponding insert. Mice immunized with the affinity-purified 55-kDa recombinant antigen produced a high titer of antibody in serum as measured by an enzyme-linked immunosorbent assay and gave a monospecific reaction by Western immunoblotting. Challenge infection of these immunized mice showed low protection from clinical infection.

show abstract

Antigenic and genetic relatedness of eight Rickettsia tsutsugamushi antigens

Cited by 55 publications

References 25 publications

Immunohistochemical Study of Scrub Typhus: A Report of Two Cases

Immunohistochemical Study of Scrub Typhus: A Report of Two Cases

Cyclic rickettsemia during persistent Anaplasma marginale infection of cattle

Molecular cloning and analysis of recombinant major antigens of Ehrlichia risticii

Contact Info

Product

Resources

About