Antigenic and biological characterisation of avian paramyxovirus type I isolates from pigeons ‐ an international collaborative study

Alexander, D. J.; Russell, Peter H.; Parsons, G.; Elzein, E. M. E. Abu; Ballouh, A; Cerník, K; Engström, B.; Fevereiro, Miguel; Fleury, Hervé; Guittet, M.; Kaleta, E. F.; Kihm, U; Kösters, J; Lomniczi, B.; J, Meister; Meulemans, G.; Nerome, Kuniaki; Petek, Marko; Pokomunski, S; B, Polten; Prip, M; Richter, R. Alexander; Sághy, E; Samberg, Y.; Spanoghe, L.; Tůmová, B

doi:10.1080/03079458508436238

Cited by 96 publications

(59 citation statements)

References 10 publications

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“…The ICPI values of 149 isolates identi® ed as PPMV-1 varied between 0.71 and 1.65, with a fairly even distribution between 0.8 and 1.5. Overall, the virulence of the PPMV-1 isolates was a little lower than that described by Alexander et al (1985a), but within the range of 0.56 to 1.85 reported in a study of 409 isolates from pigeons . Although in keeping with results obtained in other studies, the low ICPI values recorded for some isolates is surprising since these viruses can be shown to have multiple basic amino acids at the F0 cleavage site.…”

Section: Discussionmentioning

confidence: 84%

Characterization of avian paramyxovirus type 1 strains isolated in Germany during 1992 to 1996

Werner¹,

Römer-Oberdörfer²,

Köllner³

et al. 1999

Avian Pathology

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In Germany all avian paramyxoviruses (APMV) isolated in regional laboratories are collected and characterized by the National Reference Laboratory. From 1992 until 1996, 635 APMV-1 virus isolates were submitted from almost all regions. Of these viruses, 371 were isolated from chickens, 39 from other poultry, 171 from pigeons and 54 from exotic birds. All isolates were examined for virulence in intracerebral pathogenicity index (ICPI) tests, for their ability to react with a panel of monoclonal antibodies (mAb) and their thermostability. In addition, the nucleotide sequences of the cleavage site of the fusion protein of a few virus isolates were determined. Most isolates from chickens and other poultry were of the velogenic pathotype. This virus was responsible for the epizootic in 1993 to 1995 in many small ocks. The same virus was obtained from some pigeons and some exotic birds. The pathogenicity of the velogenic/epizootic virus was high with most viruses giving ICPI values of 1.8 to 1.9, and the sequences of the cleavage site of all velogenic isolates tested were closely related. However, viruses isolated at the beginning of the epizootic period differed from viruses isolated towards the end in their reaction with some mAbs. 149 virus isolates were identi® ed as pigeon variant PMV-1 (PPMV-1). Most of these were obtained from pigeons but a few were isolated from chickens and other birds. Most lentogenic isolates proved to be vaccine virus strains.

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Section: Discussionmentioning

confidence: 84%

Characterization of avian paramyxovirus type 1 strains isolated in Germany during 1992 to 1996

Werner¹,

Römer-Oberdörfer²,

Köllner³

et al. 1999

Avian Pathology

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“…Mouse monoclonal antibodies to Ulster 2C were prepared as described (Russell and Alexander, 1983;Russell et al, 1983b). These antibodies have been used to place NDV strains and isolates into nine distinct groups on the basis of the ability of the viruses to induce binding to infected MDBK cells (Russell and Alexander, 1983;Alexander et al, 1985a). The results of such studies indicated that viruses were grouped on the basis of epizootiological and biological properties and these are summarised in Table 1.…”

Section: Antiseramentioning

confidence: 99%

“…Using the groupings described by Russell and Alexander (1983) and Alexander et al (1985a) the isolates were characterised: group P -40 isolates, group C -35 isolates, group E -23 isolates, group G -2 isolates, group H -1 isolate, group L (a new binding group) -1 isolate, untyped -4 isolates.…”

Section: Vaccines and Laboratory Strainsmentioning

confidence: 99%

Use of monoclonal antibodies in the characterisation of avian paramyxovirus type 1 (Newcastle disease virus) isolates submitted to an international reference laboratory

et al. 1987

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“…mAbs may detect slight variations in antigenicity, such as single amino acid changes at the epitope to which the antibody is directed. The use of mAb technology provided a new approach to antigenic differentiation of NDV strains and isolates, and several groups developed mAbs against NDV strains, primarily for diagnostic purposes (Hoshi et al, 1983;Nishikawa et al, 1983;Russell & Alexander, 1983;Alexander et al, 1985aAlexander et al, , 1987Ishida et al, 1985;Abenes et al, 1986;Srinivasappa et al, 1986;Erdei et al, 1987;Meulemans et al, 1987;Lana et al, 1988;Jestin et al, 1989). mAbs to NDV have been effective in the following areas.…”

Section: Use Of Monoclonal Antibodies In the Diagnosis Of Ndmentioning

confidence: 99%

“…For example, two groups have described mAbs that distinguish between the common vaccine strains, Hitchner B1 and La Sota (Erdei et al, 1987;Meulemans et al, 1987), while other mAbs can separate vaccine viruses from epizootic virus in a given area (Srinivasappa et al, 1986). mAb typing was also used to establish the uniqueness of the variant NDV (PPMV-1) responsible for the pigeon panzootic and has proven particularly useful in identifying the spread of this virus around the world (Alexander et al, 1985aPearson et al, 1987). During the epizootic of NDV in Great Britain in 1984, rapid identificatio n of the virus as PPMV-1 enabled early tracing of the source of disease to contaminated feed ingredients, and subsequent control (Alexander et al, 1985b).…”

Section: Identification Of Specific Virusesmentioning

confidence: 99%

Detection and differentiation of Newcastle disease virus (avian paramyxovirus type 1)

2001

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Substantial variation in the virulence of Newcastle disease virus (NDV) isolates means that the detection of NDV or evidence of infection is insufficient for an adequate diagnosis, as control measures for avirulent viruses are very different to those for virulent viruses. Diagnosis therefore requires further characterization, at least as to whether an isolate is virulent or avirulent. Conventional detection and differentiation of ND viruses is perceived as slow, laborious and requiring an undesirable use of in vivo techniques. In addition, further characterization is needed to give greater information on origin and spread. This review concentrates on the application of monoclonal antibody and molecular biological approaches. Panels of monoclonal antibodies were a major advance for the characterization of NDV isolates, although confirmation of virulence for poultry still required in vivo testing. As molecular-based techniques become easier and more reliable, they are likely to supersede the use of monoclonal antibodies, especially for characterizing viruses for epidemiological purposes. The attraction of molecular-based techniques is that they may be able to cover all three aspects of Newcastle disease diagnosis (detection of virus, characterization, including inference of virulence, and epidemiology) quickly, accurately and definitively in a single test. A number of approaches based on the reverse transcriptase polymerase chain reaction have been developed, with subsequent analysis of the product by restriction enzyme analysis, probe hybridization and nucleotide sequencing. Although extensive variation among NDVs still poses technical problems, the real and potential advantages of a molecular biological approach to Newcastle disease diagnosis appear to be overwhelming.

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Antigenic and biological characterisation of avian paramyxovirus type I isolates from pigeons ‐ an international collaborative study

Cited by 96 publications

References 10 publications

Characterization of avian paramyxovirus type 1 strains isolated in Germany during 1992 to 1996

Characterization of avian paramyxovirus type 1 strains isolated in Germany during 1992 to 1996

Use of monoclonal antibodies in the characterisation of avian paramyxovirus type 1 (Newcastle disease virus) isolates submitted to an international reference laboratory

Detection and differentiation of Newcastle disease virus (avian paramyxovirus type 1)

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