Antigenic Analysis of Japanese Encephalitis Virus Using Monoclonal Antibodies

Kobayashi, Yoshinari; Hasegawa, Hitoshi; Oyama, Takashi; Tamai, Tomonori; Shiroguchi, Tomoo; Kusaba, Tomohiro

doi:10.11150/kansenshogakuzasshi1970.57.519

Cited by 16 publications

(19 citation statements)

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“…It is noteworthy that the B domain was absent from the JE Nakayama-Biken vaccine strain but present in another Nakayama strain obtained from the Rockefeller Foundation. Differences in the immunological characteristics of various Nakayama substrains have been reported (Kobayashi et al, 1984) and we are currently looking into the significance of our observations to see if protection is affected by these changes.…”

Section: Discussionmentioning

confidence: 99%

Epitope Mapping of Japanese Encephalitis Virus Envelope Protein Using Monoclonal Antibodies against an Indian Strain

Cecilia

Gadkari

Kedarnath

et al. 1988

Journal of General Virology

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SUMMARYA panel of monoclonal antibodies (MAbs) raised against an Indian strain of Japanese encephalitis (JE) virus was used to map topographically the epitopes on the envelope protein. Two separate clusters of epitopes were revealed. On the basis of reactivity in haemagglutination inhibition (HI), neutralization (NT), passive protection and antibody-dependent plaque enhancement (ADPE) assays with the MAbs, five functional domains (A, B, C, D and E) were delineated. The flavivirus cross-reactive domain for HI (A) was distinct. The JE virus-specific domain for HI (B) was in continuum with those domains representing non-HI JE virus-specific MAbs (C) and flavivirus cross-reactive MAbs (D). Domain E, which mapped close to domain D was represented by two MAbs that reacted with both JE virus and uninfected cell nuclei. Four conclusions can be drawn. (i) Two distinct antigenic domains were associated with HI, (ii) HI and NT in vivo and in vitro were dissociated functions, (iii) ADPE activity was solely linked with the A domain and (iv) all MAbs reacting with epitopes in the B domain had HI/NT/protective activity but failed to show ADPE. The B domain might therefore be considered the most suitable for development of synthetic or genetically engineered vaccines.

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Section: Discussionmentioning

confidence: 99%

Epitope Mapping of Japanese Encephalitis Virus Envelope Protein Using Monoclonal Antibodies against an Indian Strain

Cecilia

Gadkari

Kedarnath

et al. 1988

Journal of General Virology

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“…The number of infected monocytes was determined by immunoperoxidase staining using horseradish peroxidase (HRPO)-labeled F(ab')2 fragment of NARMA 7 (JE species-specific monoclonal antibody) (14) at 48 hr after infection as described previously (15) . The infected monocytes were air-dried on glass slides and fixed with acetone at -20 C for 10 min, and reacted with HRPO-F (ab') 2 fragment of NARMA 7 at 37 C for 1 hr.…”

Section: Methodsmentioning

confidence: 99%

Effect of Cytokines on Japanese Encephalitis Virus Production by Human Monocytes

Hasegawa

Satake

Kobayashi

1990

Microbiology and Immunology

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“…The reason(s) for these two distinct patterns is unknown, but one possible explanation is that there are regional differences in virulence among JE virus strains. Antigenic and biochemical differences between JE virus isolates have been demonstrated by several techniques, including cross-neutralization (Banerjee, 1986), oligonucleotide fingerprinting of virion RNA (Hori et al, 1986;Banerjee & Ranadive, 1989) and reactivity with mouse monoclonal antibodies (Hasegawa, 1982;Kimura-Kuroda & Yasui, 1983;Kobayashi et al, 1984).…”

Section: Introductionmentioning

confidence: 99%

Genetic Variation of Japanese Encephalitis Virus in Nature

Chen

Tesh

Rico-Hesse

1990

Journal of General Virology

181

157

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Forty-six strains of Japanese encephalitis (JE) virus from a ovariety of geographic areas in Asia were examined by primer-extension sequencing of the RNA template. A 240 nucleotide sequence from the pre-M gene region was selected for study because it provided sufficient information for determining genetic relationships among the virus isolates. Using 12 % divergence as a cutoff point for virus relationships, the 46 isolates fell into three distinct genotypic groups. One genotypic group consisted of JE virus isolates from northern Thailand and Cambodia. A second group was composed of isolates from southern Thailand, Malaysia, Sarawak and Indonesia. The remainder of the isolates, from Japan, China, Taiwan, the Philippines, Sri Lanka, India and Nepal, made up a third group. The implications of these findings in relation to the epidemiology of JE are discussed. Results of this study demonstrate that the comparison of short nucleotide sequences can provide insight into JE virus evolution, transmission and, possibly, pathogenesis.

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Antigenic Analysis of Japanese Encephalitis Virus Using Monoclonal Antibodies

Cited by 16 publications

References 0 publications

Epitope Mapping of Japanese Encephalitis Virus Envelope Protein Using Monoclonal Antibodies against an Indian Strain

Epitope Mapping of Japanese Encephalitis Virus Envelope Protein Using Monoclonal Antibodies against an Indian Strain

Effect of Cytokines on Japanese Encephalitis Virus Production by Human Monocytes

Genetic Variation of Japanese Encephalitis Virus in Nature

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