Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells.

Abstract

The anti-My-10 mouse monoclonal antibody was raised against the immature human myeloid cell line KG-1a and was selected for nonreactivity with mature human granulocytes. Anti-My-10 immunoprecipitated a KG-1a cell surface protein with an apparent Mr of approximately 115 kD. We describe the binding of this antibody to human hematopoietic cell types and show that My-10 is expressed specifically on immature normal human marrow cells, including hematopoietic progenitor cells. My-10 is also expressed by leukemic marrow cells from a subpopulation of patients. Thus, this antibody allows the identification and purification of hematopoietic progenitor cells from normal human marrow and the subclassification of leukemia.

Topical brimonidine for the management of facial erythema in rosacea: a histological, histometric, and immunohistochemical study

“…A transmembrane phosphoglycoprotein (CD34) is predominantly regarded as a marker of hematopoietic stem cells (HSC) and hematopoietic progenitor cells. However, it is also established as a marker of other non‐hematopoietic cell types, including vascular endothelial progenitors and embryonic fibroblasts 26,27 . Clinical improvement in response to topical brimonidine tartrate eye drops (0.2%) treatment in the present work was consistent with the histological and immunohistochemical results.…”

Synthetic oleanane triterpenoids suppress MYB oncogene activity and sensitize T-cell acute lymphoblastic leukemia cells to chemotherapy

“…Similarly, we found that genes involved in positive regulation of differentiation were upregulated in T-ALL cells following treatment with bardoxolone methyl or omaveloxolone. Since the hematopoietic progenitor marker CD34 ( 51 ) was downregulated by both OTs and MYB knockdown, this suggests that the differentiation seen following OT treatment is, at least partly, mediated by decreased MYB expression in T-ALL cells. This conclusion is further supported by studies showing that MYB expression is high in immature and progenitor-like cells and suppress differentiation of both normal and leukemic cells ( 7 , 26 ).…”

Deciphering postnatal limb development at single-cell resolution

“…In the present study, two sub-clusters of candidate articular cartilage progenitors featured by Cd34 or Ly6e / Ly6a were discovered. Cd34 was first described on hematopoietic stem and progenitor cells, 111 and recently, considerable studies have demonstrated that Cd34 is expressed by a multitude of other non-hematopoietic cell types including fibrogenic/adipogenic progenitors, 112 muscle satellite cells, 113 corneal keratocytes, interstitial cells, epithelial progenitors, and vascular endothelial progenitors. 114 It shows that Cd34 + cells discovered in various tissues are usually characterized with enhanced progenitor features.…”