Antigènes nouveaux dans les cellules transformées par le SV40. I. Mise en évidence de trois nouveaux constituants antigéniques dans plusieurs lignées cellulaires d'origines différentes transformées par le SV40

A clone of hamster fibroblasts tramformed by SV40 virus (TSVKCL,) induced antibodies in hamsters against new antigenic constituents not present in the untransformed fibroblasts. The antibodies in the immune sera against histocompatibility antigens and those against nuclear T antigen were absorbed. The absorbed sera contained antibodies which agglutinated the transformed cells. The sera contained also precipitatiitg antibodies against new antigens present in a crude extract of TSVKCL2. These antibodies were directed to common new antigens present in SV40-transformed fibroblasts from different species. ImmunoJluorescence and the '' indirect enzyme-labelled antibody technique '' were used for the cellular localization of the antigens corresponding to the precipitating antibodies. Both techniques showed that these antigens were localized in the cytoplasm, mostly in the microsome-ribosomal fractions, much less on the cell membrane and not at all in the nucleus.

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Study of the in vivo fixation of antibodies on tumors provoked in hamsters by injection of SV40‐transformed cells (TSV₅Cl₂)

Sobczak

Cyr

1971

Intl Journal of Cancer

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Large particle fraction obtained by the technique of Sjögren and Motet (1969) can be treated at acid pH, to elute immunoglobulins from hamster tumors induced by injection of SV‐40 transformed fibroblasts (TSV5Cl2). The eluate contains IgG27S, as well as two fragments having molecular weights of about 67,000; one of these, Fc, migrates in the β region, and the other, F(ab), migrates in the slow y region. This eluate, tested on TSV5Cl2in culture, contains some cytotoxic antibodies and antibodies against T antigen and cytoplasmic antigen.

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Antigènes nouveaux dans les cellules transformées par le SV40. I. Mise en évidence de trois nouveaux constituants antigéniques dans plusieurs lignées cellulaires d'origines différentes transformées par le SV40

Cited by 10 publications

References 11 publications

Virus‐Induced, Tumour‐Associated Antigens

Virus‐Induced, Tumour‐Associated Antigens

New antigens in cells transformed by the SV40 virus. II. Evidence of their cytoplasmic localization in a cell line (TSV₅ CL₂) of hamster fibroblasts transformed by SV40

Study of the in vivo fixation of antibodies on tumors provoked in hamsters by injection of SV40‐transformed cells (TSV₅Cl₂)

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Antigènes nouveaux dans les cellules transformées par le SV40. I. Mise en évidence de trois nouveaux constituants antigéniques dans plusieurs lignées cellulaires d'origines différentes transformées par le SV40

Cited by 10 publications

References 11 publications

Virus‐Induced, Tumour‐Associated Antigens

Virus‐Induced, Tumour‐Associated Antigens

New antigens in cells transformed by the SV40 virus. II. Evidence of their cytoplasmic localization in a cell line (TSV5 CL2) of hamster fibroblasts transformed by SV40

Study of the in vivo fixation of antibodies on tumors provoked in hamsters by injection of SV40‐transformed cells (TSV5Cl2)

Contact Info

Product

Resources

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New antigens in cells transformed by the SV40 virus. II. Evidence of their cytoplasmic localization in a cell line (TSV₅ CL₂) of hamster fibroblasts transformed by SV40

Study of the in vivo fixation of antibodies on tumors provoked in hamsters by injection of SV40‐transformed cells (TSV₅Cl₂)